Structure of Extracellular Polysaccharides of Escherichia coli Strains 36M, 72M, and 29M Isolated from Coligranuloma of Chick Intestine

Kamei, Akira; Takeuchi, Noriko; Akashi, Shuzo; Kagabe, Koshiro

doi:10.1093/oxfordjournals.jbchem.a131989

Cited by 2 publications

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“…The depyruvylated preparation was obtained by refluxing a 0.1 "/, homogenate of the deionized native polysaccharide in 1 M HCI at 100 ~C for 1 h. After neutralization with NaOH, the product was recovered by ethanol precipitation. (d) Ketal exchange reaction with acetone according to Kamei et al [21]. Fully methylated native polysaccharide (300 mg) suspended in acetone/ petroleum ether (1 : 1, vjv, 20 ml) was refluxed with p-toluenesulfonic acid (200 mg) for 48 h. The product (13 mg) was purified and identified as methyl pyruvate.…”

Section: Identification Of Pyruvic Acidmentioning

confidence: 99%

An Extracellular Polysaccharide Produced by Zoogloea ramigera 115

Ikeda

Shuto

Saito

et al. 1982

European Journal of Biochemistry

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A weakly acidic polysaccharide was purified from the extracellular zoogloeal matrix produced by Zoogloeal ramigera 115. The purified polysaccharide was homogeneous as judged by sedimentation analysis, and the average molecular weight was estimated to be about lo5 by gel permeation chromatography of the fully methylated preparation. The polysaccharide was composed of D-glucose, D-gahCtOSe and pyruvic acid in an approximate molar ratio of 11 : 3 : 1.5. On the basis of methylation, periodate oxidation, Smith degradation and partial hydrolysis, the following highly branched structure was deduced for the polysaccharide : a long chain mainly consisting of fl1-+4-linked glucose residues branching at the C-3 or C-6 position of galactose residues which are present in D l -+ 4 or P I + 3 linkages as the minor component of the long chain; pyruvic acid residues, the sole acidic component, are linked to the nonreducing end and/or 1,3-linked glucose residues through 4,6-ketal linkages. The purified polysaccharide was not readily soluble in water and had a high affinity for several metallic ions (e.g. 0.25 pmol Fe3+/mg, and 0.17 pmol Fe2+ mg). Upon addition of metallic ions (1 mM) to a gelatinous aqueous solution of the polysaccharide (K' form, 0.125 %), more than 80% of it immediately coprecipitated out with them.Among a group of floc-forming bacteria indigenous to activated sludges or organically polluted water, Zoogloea ramigera I-16-M produces no gelatinous matrix [l], while Zoogloea ramigera 1 15, isolated by Friedman and Dugan, produces an extracellular zoogloeal matrix in which the cells are embedded [2]. Although the gelatinuous matrix polymer is known to have a remarkably high affinity for several metallic ions [ 3 ] and organic compounds such as amino acids [4], its chemical nature has not been fully elucidated yet. Dugan and his coworkers [5,6] reported that the extracellular polymer was composed of fibrillar strands of polysaccharide which consisted of glucose and galactose, and also contained carboxyl functions or terminal aldehyde groups. The purpose of this study was to investigate further the chemical structure of the extracellular polysaccharide purified from Z. ramigera 11 5 , which forms a gelatinous aqueous solution and shows a high affinity for several metallic ions. EXPERIMENTAL PROCEDURES Culture of OrganismZoogloea ramigera 11 5, kindly provided by Dr P. R. Dugan (Ohio State University, Columbus, OH, USA) was maintained on Trypticase soy agar slants at room temperature. The bacterium was grown on a reciprocal shaker at 30°C for 72 h in a basal medium (500 ml in 2000-ml Sakaguchi flasks) containing 0.1 % casamino acids, 0.05 % yeast extract, 0.1 % K2HP04, 0.05 % KH2P04 and 30 mM glucose, until the culture liquor became very viscous.

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Section: Identification Of Pyruvic Acidmentioning

confidence: 99%