Structure of Phycobilisomes

Sui, Sen‐Fang

doi:10.1146/annurev-biophys-062920-063657

Cited by 38 publications

(33 citation statements)

References 99 publications

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“…Cyanobacteria are a large group of prokaryotes that perform oxygenic photosynthesis, and they play important roles in global carbon and nitrogen cycles. Phycobilisomes (PBS) are supramolecular pigment–protein complexes composed of phycobiliproteins and linker proteins and they are the major antenna complexes for light energy harvesting in cyanobacteria [ 1 , 2 , 3 , 4 , 5 ]. There are two types of PBS in most cyanobacteria: hemidiscoidal PBS and CpcL-PBS.…”

Section: Introductionmentioning

confidence: 99%

Attachment of Ferredoxin: NADP+ Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium Synechococcus sp. PCC 7002

Huang

Wei

et al. 2022

Microorganisms

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Two types of cyanobacterial phycobilisomes (PBS) are present: the hemidiscoidal PBS (CpcG-PBS) and the membrane-bound PBS (CpcL-PBS). Both types of PBS have ferredoxin:NADP+ oxidoreductase (FNR) attached to the termini of their rods through a CpcD domain. To date, the physiological significance of the attachment remains unknown. We constructed a mutant (dF338) which contains an FNR lacking the N-terminal CpcD domain in Synechococcus sp. PCC 7002. Isolated CpcG-PBS from dF338 did not contain FNR and the cell extracts of the mutant had a 35 kDa protein cross-reacting to anti-FNR antibodies. dF338 grows normally under photoautotrophic conditions, but little growth was observed under photoheterotrophic conditions. A cpcL (cpcG2) mutant grows extremely slowly under photoheterotrophic conditions while a cpcG (cpcG1) mutant, in which PBS rods could not attach to the cores of the CpcG-PBS, can grow photoheterotrophically, strongly suggesting that the attachment of FNR to CpcL-PBS is critical to photoheterotrophic growth. We show that electron transfer to the plastoquinone pool in dF338 and the cpcL mutant was impaired. We also provide evidence that trimeric photosystem I (PSI) and intact CpcL-PBS with a full-length FNR is critical to plastoquinone reduction. The presence of a NADPH-dehydrogenase (NDH)-CpcL-PBS-PSI trimer supercomplex and its roles are discussed.

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Section: Introductionmentioning

confidence: 99%

Attachment of Ferredoxin: NADP+ Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium Synechococcus sp. PCC 7002

Huang

Wei

et al. 2022

Microorganisms

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“…Moreover, Kuddus et al [46] outlined the C-phycocyanin production and biotechnological applications. Sui [47] summarized the structure of phycobilisomes, while Silva et al [48] reviewed the mechanisms of action and multidrug resistance of phycocyanin in cancer. However, there is still a paucity of information on the present, current, and future research trends of phycobiliproteins.…”

Section: Overview Of Previous Phycobiliprotein Researchmentioning

confidence: 99%

Uncovering Research Trends of Phycobiliproteins Using Bibliometric Approach

Tan

Yusoff

Khaw

et al. 2021

Plants

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Phycobiliproteins are gaining popularity as long-term, high-value natural products which can be alternatives to synthetic products. This study analyzed research trends of phycobiliproteins from 1909 to 2020 using a bibliometric approach based on the Scopus database. The current findings showed that phycobiliprotein is a burgeoning field in terms of publications outputs with “biochemistry, genetics, and molecular biology” as the most related and focused subject. The Journal of Applied Phycology was the most productive journal in publishing articles on phycobiliproteins. Although the United States of America (U.S.A.) contributed the most publications on phycobiliproteins, the Chinese Academy of Sciences (China) is the institution with the largest number of publications. The most productive author on phycobiliproteins was Glazer, Alexander N. (U.S.A.). The U.S.A. and Germany were at the forefront of international collaboration in this field. According to the keyword analysis, the most explored theme was the optimization of microalgae culture parameters and phycobiliproteins extraction methods. The bioactivity properties and extraction of phycobiliproteins were identified as future research priorities. Synechococcus and Arthrospira were the most cited genera. This study serves as an initial step in fortifying the phycobiliproteins market, which is expected to exponentially expand in the future. Moreover, further research and global collaboration are necessary to commercialize phycobiliproteins and increase the consumer acceptability of the pigments and their products.

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“…Phycocyanin is a pigment-protein complex from the light-harvesting phycobiliprotein family, along with allophycocyanin and phycoerythrin, and is an accessory pigment [70]. All phycobiliproteins are water-soluble, in contrast to carotenoids that are hydrophobic and form clusters that adhere to the membrane called phycobilisomes.…”

Section: Pigmentsmentioning

confidence: 99%

“…Polyunsaturated fatty acids are undesirable components of fuel, as they are more prone to oxidation. Therefore, leading to the production of short chain aldehydes and ketones can have corrosive enhancing properties [68,70]. The oxidative stability of 18:2, 18:3, 20:4, and 22:6 is linearly related to the number of bis-allylic motifs and reveals that oxidisability increases approximately 2-fold for each active bis-allylic methylene group, thus 22:6 is 5 times greater than 18:2 [75].…”

Section: Algal Oils and Biodiesel Productionmentioning

confidence: 99%

“…This could be achieved by genetic manipulation and downregulation of the genes involved in PUFA synthesis. Interestingly, the enzymes involved in synthesising PUFAs (elongases and desaturases) are decreased in response to nitrogen limitation [71] and indeed stress appears to lower PUFA production in algae in general [70]. By contrast, low temperature appears to favour the synthesis of more highly unsaturated fatty acids in algae [68].…”

Section: Algal Oils and Biodiesel Productionmentioning

confidence: 99%

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Key Targets for Improving Algal Biofuel Production

et al. 2021

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A number of technological challenges need to be overcome if algae are to be utilized for commercial fuel production. Current economic assessment is largely based on laboratory scale up or commercial systems geared to the production of high value products, since no industrial scale plant exits that are dedicated to algal biofuel. For macroalgae (‘seaweeds’), the most promising processes are anaerobic digestion for biomethane production and fermentation for bioethanol, the latter with levels exceeding those from sugar cane. Currently, both processes could be enhanced by increasing the rate of degradation of the complex polysaccharide cell walls to generate fermentable sugars using specifically tailored hydrolytic enzymes. For microalgal biofuel production, open raceway ponds are more cost-effective than photobioreactors, with CO2 and harvesting/dewatering costs estimated to be ~50% and up to 15% of total costs, respectively. These costs need to be reduced by an order of magnitude if algal biodiesel is to compete with petroleum. Improved economics could be achieved by using a low-cost water supply supplemented with high glucose and nutrients from food grade industrial wastewater and using more efficient flocculation methods and CO2 from power plants. Solar radiation of not <3000 h·yr−1 favours production sites 30° north or south of the equator and should use marginal land with flat topography near oceans. Possible geographical sites are discussed. In terms of biomass conversion, advances in wet technologies such as hydrothermal liquefaction, anaerobic digestion, and transesterification for algal biodiesel are presented and how these can be integrated into a biorefinery are discussed.

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Structure of Phycobilisomes

Cited by 38 publications

References 99 publications

Attachment of Ferredoxin: NADP+ Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium Synechococcus sp. PCC 7002

Attachment of Ferredoxin: NADP+ Oxidoreductase to Phycobilisomes Is Required for Photoheterotrophic Growth of the Cyanobacterium Synechococcus sp. PCC 7002

Uncovering Research Trends of Phycobiliproteins Using Bibliometric Approach

Key Targets for Improving Algal Biofuel Production

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