Structure of the 4-hydroxy-tetrahydrodipicolinate synthase from the thermoacidophilic methanotroph <i>Methylacidiphilum fumariolicum</i> SolV and the phylogeny of the aminotransferase pathway

Schmitz, Rob A.; Dietl, Andreas; Müller, Melanie; Berben, Tom; Camp, Huub J. M. Op den; Barends, Thomas R. M.

doi:10.1107/s2053230x20005294

Cited by 4 publications

(3 citation statements)

References 52 publications

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“…acquired by residents of hypersaline environment for normal growth and development of cells were observed to be prevalent in our study. These genes include ABC-2 type transport system permease protein (K01992), ABC-2 type transport system ATP-binding protein (K01990), ATP-binding cassette, subfamily B, bacterial (K06147), 1-acyl-sn-glycerol-3-phosphate acyltransferase (K00655), 4-hydroxy-tetrahydrodipicolinate synthase (K01714), and DNA helicase II / ATP-dependent DNA helicase PcrA (K03657) ( Sleator and Hill, 2002 ; Yu et al, 2018 ; Schmitz et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…acquired by residents of hypersaline environment for normal growth and development of cells were observed to be prevalent in our study. These genes include ABC-2 type transport system permease protein (K01992), ABC-2 type transport system ATP-binding protein (K01990), ATP-binding cassette, subfamily B, bacterial (K06147), (Sleator and Hill, 2002;Yu et al, 2018;Schmitz et al, 2020). Salt pans in Goa are influenced by three seasons: pre-monsoon, monsoon, and post-monsoon, where salinity ranges from 30-300 PSU (Mani et al, 2012).…”

Section: Significance Of Halophilesmentioning

confidence: 99%

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Bacterial diversity and community structure of salt pans from Goa, India

Gawas,

Kerkar

2023

Front. Microbiol.

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In Goa, salt production from the local salt pans is an age-old practice. These salt pans harbor a rich diversity of halophilic microbes with immense biotechnological applications, as they tolerate extremely harsh conditions. Detecting the existence of these microbes by a metabarcoding approach could be a primary step to harness their potential. Three salt pans viz. Agarwado, Curca, and Nerul adjoining prominent estuaries of Goa were selected based on their unique geographical locations. The sediments of these salt pans were examined for their bacterial community and function by 16S rRNA amplicon-sequencing. These salt pans were hypersaline (400–450 PSU) and alkaline (pH 7.6–8.25), with 0.036–0.081 mg/L nitrite, 0.0031–0.016 mg/L nitrate, 6.66–15.81 mg/L sulfate, and 20.8–25.6 mg/L sulfide. The relative abundance revealed that the Pseudomonadota was dominant in salt pans of Nerul (13.9%), Curca (19.6%), and Agarwado (32.4%). The predominant genera in Nerul, Curca, and Agarwado salt pan sediments were Rhodopirellula (1.12%), Sulfurivermis (1.28%), and Psychrobacter (25.5%) respectively. The highest alpha diversity (Shannon-diversity Index) was observed in the Nerul salt pan (4.8) followed by Curca (4.3) and Agarwado (2.03). Beta diversity indicated the highest dissimilarity between Agarwado and the other two salt pans (0.73) viz. Nerul and Curca and the lowest dissimilarity was observed between Nerul and Curca salt pans (0.48). Additionally, in the Agarwado salt pan, 125 unique genera were detected, while in Nerul 119, and in Curca 28 distinct genera were noted. The presence of these exclusive microorganisms in a specific salt pan and its absence in the others indicate that the adjacent estuaries play a critical role in determining salt pan bacterial diversity. Further, the functional prediction of bacterial communities indicated the predominance of stress adaptation genes involved in osmotic balance, membrane modification, and DNA repair mechanisms. This is the first study to report the bacterial community structure and its functional genes in these three salt pans using Next-Generation Sequencing. The data generated could be used as a reference by other researchers across the world for bioprospecting these organisms for novel compounds having biotechnological and biomedical potential.

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Section: Discussionmentioning

confidence: 99%

“…acquired by residents of hypersaline environment for normal growth and development of cells were observed to be prevalent in our study. These genes include ABC-2 type transport system permease protein (K01992), ABC-2 type transport system ATP-binding protein (K01990), ATP-binding cassette, subfamily B, bacterial (K06147), (Sleator and Hill, 2002;Yu et al, 2018;Schmitz et al, 2020). Salt pans in Goa are influenced by three seasons: pre-monsoon, monsoon, and post-monsoon, where salinity ranges from 30-300 PSU (Mani et al, 2012).…”

Section: Significance Of Halophilesmentioning

confidence: 99%

Bacterial diversity and community structure of salt pans from Goa, India

Gawas,

Kerkar

2023

Front. Microbiol.

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“…Although monobactam is a monocyclic beta-lactam antibiotic widely used against bacteria, the enzymes encoded by the DEGs in this pathway (i.e., aspartate kinase and 4-hydroxy-tetrahydropyridine dicarboxylate synthase) (Fig. 4 D) are also rate-limiting enzymes during aspartate synthesis [ 38 ] and lysine synthesis [ 39 ]. Monobactam biosynthesis combined with the production of aspartic acid and lysine may be involved in the mechanism underlying the resistance to web blotch.…”

Section: Discussionmentioning

confidence: 99%

Cytological and transcriptomic analysis to unveil the mechanism of web blotch resistance in Peanut

Wu,

Sun,

et al. 2023

BMC Plant Biol

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Background Peanut is an important oil crop worldwide. Peanut web blotch is a fungal disease that often occurs at the same time as other leaf spot diseases, resulting in substantial leaf drop, which seriously affects the peanut yield and quality. However, the molecular mechanism underlying peanut resistance to web blotch is unknown. Results The cytological examination revealed no differences in the conidium germination rate between the web blotch-resistant variety ZH and the web blotch-susceptible variety PI at 12–48 hpi. The appressorium formation rate was significantly higher for PI than for ZH at 24 hpi. The papilla formation rate at 36 hpi and the hypersensitive response rate at 60 and 84 hpi were significantly higher for ZH than for PI. We also compared the transcriptional profiles of web blotch-infected ZH and PI plants at 0, 12, 24, 36, 48, 60, and 84 hpi using an RNA-seq technique. There were more differentially expressed genes (DEGs) in ZH and PI at 12, 36, 60, and 84 hpi than at 24 and 48 hpi. Moreover, there were more DEGs in PI than in ZH at each time-point. The analysis of metabolic pathways indicated that pantothenate and CoA biosynthesis; monobactam biosynthesis; cutin, suberine and wax biosynthesis; and ether lipid metabolism are specific to the active defense of ZH against YY187, whereas porphyrin metabolism as well as taurine and hypotaurine metabolism are pathways specifically involved in the passive defense of ZH against YY187. In the protein-protein interaction (PPI) network, most of the interacting proteins were serine acetyltransferases and cysteine synthases, which are involved in the cysteine synthesis pathway. The qRT-PCR data confirmed the reliability of the transcriptome analysis. Conclusion On the basis of the PPI network for the significantly enriched genes in the pathways which were specifically enriched at different time points in ZH, we hypothesize that serine acetyltransferases and cysteine synthases are crucial for the cysteine-related resistance of peanut to web blotch. The study results provide reference material for future research on the mechanism mediating peanut web blotch resistance.

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