Structure of the bovine pancreatic ribonuclease gene: the unique intervening sequence in the 5′ untranslated region contains a promoter-like element

Abstract

Although pancreatic ribonucleases are extensively studied proteins, little information is available on nucleic acids coding for these enzymes. Here, for the first time, the structure of a gene coding for such an enzyme, the well known bovine pancreatic ribonuclease, is reported. The coding region of this gene is devoid of introns, whereas the 5' untranslated sequence of the pancreatic transcript contains an intron of 735 nucleotides. This intervening sequence is endowed with signals (CAAT and TATA boxes) which might act as regulatory elements. The structural organization of this gene suggests that the sequence coding for the bovine pancreatic ribonuclease might be expressed under the control of two different promoters.

Ribonuclease activity and substrate preference of human eosinophil cationic protein (ECP)

“…These observations further emphasize the confusion generated by use of the terms secretory and non-secretory when all members of the RNase gene superfamily encode signal peptides [10,32] which may lead to secretion or to localization in lysosomes. Perhaps designation of ECP as a liver type RNase or, as suggested by Maddalena et al [33], as a member of the RNase IIu subclass is more logical.…”

Pseudogenes in ribonuclease evolution: a source of new biomacromolecular function?

“…Sequences of the genes and the encoded polypeptide chain (using the one letter code) of members of the pancreatic and seminal RNase families. Published RNase sequences are from giraffe [21], ox pancreas [19] and ox seminal plasma [20]. million years before present (approximate) Fig.…”

“…PCR reaction conditions: 1 min 95°C, 2 rain 52°C, 50 s 72°C, 3 cycles and 8 min 72°C [23]. The PCR fragments of saiga and kudu were cloned in a pUC19 plasmid using T4 DNA Ligase (NEB) in a 1 × ligation buffer: 50 rnM Tris/ HC1 (pH 7.8), 10 mM MgC12, 10 mM DTT, 1 mM ATP, 25 ~g/ml BSA at 14°C overnight [19]. The PCR products were analysed either by direct sequencing (USB-kit) or by start and reverse primers (Synthesizer ABI, 380A) [24].…”

“…[17,18]. These complement the genes for ox pancreatic RNase [19], seminal RNase from ox (Bos taurus) [20], and giraffe (Giraffa camelopardalis) [21] and hog deer (Beintema, personal communication) (Figs. 1 and 2).…”

Intronic Enhancer Activity of the Eosinophil-derived Neurotoxin (RNS2) and Eosinophil Cationic Protein (RNS3) Genes Is Mediated by an NFAT-1 Consensus Binding Sequence

“…The genes encoding EDN and ECP are 90% homologous to one another and both include two exons; each gene contains a noncoding exon 1, separated by a single intron from the coding sequence in exon 2. This gene structure is characteristic of the ribonuclease gene family (3)(4)(5)(6). In our previous work, we have demonstrated that optimal expression of the EDN gene is dependent on interaction between the 5Ј promoter region and the single intron (7).…”