Structure of the hypothetical DUF1811-family protein GK0453 fromGeobacillus kaustophilusHTA426

Abstract

The crystal structure of a conserved hypothetical protein, GK0453, from Geobacillus kaustophilus has been determined to 2.2 Å resolution. The crystal belonged to space group P4 3 2 1 2, with unit-cell parameters a = b = 75.69, c = 64.18 Å . The structure was determined by the molecular-replacement method and was refined to a final R factor of 22.6% (R free = 26.3%). Based on structural homology, the GK0453 protein possesses two independent binding sites and hence it may simultaneously interact with two proteins or with a protein and a nucleic acid.