Structure of the K10 capsular antigen from Escherichia coli O11:K10:H10, a polysaccharide containing 4,6-dideoxy-4-malonylamino-d-glucose

Sieberth, Veit; Jann, Barbara; Jann, Klaus

doi:10.1016/0008-6215(93)84034-4

Cited by 10 publications

(6 citation statements)

References 17 publications

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“…The absence of kpsU is consistent with the lack of elevated levels of CMP-KDO synthetase found in group III-expressing strains (18). The identification of homologues to the KpsC and KpsS proteins is in keeping with the observation that phosphatidyl-KDO has been detected at the reducing end of the K10 polysaccharide (39) and would be in agreement with the suggested role of the KpsC and KpsS proteins in the addition of phosphatidyl-KDO to the reducing end of polysaccharide (34). The lack of a capsulespecific CMP-KDO synthetase enzyme would suggest that the CMP-KDO for group III capsule expression is supplied by the KdsB enzyme (11,28).…”

supporting

confidence: 82%

“…The rmlC gene encodes dTDP-4keto-6-deoxy-D-glucose-3,5-epimerase, which is required for synthesis of an intermediate in the dTDP-rhamnose pathway (47). The E. coli K10 capsule contains rhamnose as one of its component sugars (39), and so this RmlC homologue is probably involved in the synthesis of dTDP-rhamnose required for biosynthesis of the K10 polymer. Therefore, rmlC K10 defines the boundary between the serotype-specific region 2 and a conserved region 3 involved in capsule export.…”

mentioning

confidence: 99%

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Genetic Organization of the Escherichia coli K10 Capsule Gene Cluster: Identification and Characterization of Two Conserved Regions in Group III Capsule Gene Clusters Encoding Polysaccharide Transport Functions

1999

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Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K10 capsule production, suggesting that expression of the K10 capsule was regulated by RfaH in a manner analogous to group II capsule gene clusters. An IS3 element and a φR73-like prophage, both of which may have played a role in the acquisition of group III capsule gene clusters, were detected flanking the K10 capsule genes.

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supporting

confidence: 82%

mentioning

confidence: 99%

Genetic Organization of the Escherichia coli K10 Capsule Gene Cluster: Identification and Characterization of Two Conserved Regions in Group III Capsule Gene Clusters Encoding Polysaccharide Transport Functions

1999

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“…Second, antibody and bacteriophage specific for the K10 capsule were available, facilitating isolation of K10-expressing clones. Finally, the structure of the K10 capsule was known (36). A cosmid library of E. coli D1114 chromosomal DNA was constructed in E. coli LE392 and screened by colony immunoblotting with polyclonal antiserum to the K10 capsular polysaccharide.…”

Section: Resultsmentioning

confidence: 99%

Cloning and analysis of gene clusters for production of the Escherichia coli K10 and K54 antigens: identification of a new group of serA-linked capsule gene clusters

Pearce

Roberts

1995

J Bacteriol

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The polysaccharide capsules of Escherichia coli have been classified into three groups: I, II, and I/II. The third group, I/II, has been poorly studied and possesses characteristics of both group I and group II capsules. In this report, we describe the cloning of the K10 and K54 capsule gene clusters, two representatives of group I/II capsules. Probes taken from DNA flanking regions 1 and 3 of the group II capsule clusters hybridized to these group I/II clones, confirming that the group I/II capsule genes are flanked by the same DNA and are therefore located in the same serA-linked region of the chromosome as group II capsule gene clusters. Southern blotting showed that homologous sequences were present in both the K10 and K54 capsule gene clusters and in other group I/II strains. No homology was detected between these sequences and the chromosomal DNA of either a group I or a group II strain. Likewise, no homology was detected to the chromosomal DNA of either a K11 or K19 strain, both of which had previously been classified as group I/II strains. In the K10 and K54 capsule gene clusters, these conserved sequences flanked a serotype-specific region in a manner analogous to group II capsule gene organization. Complementation of mutations in the kpsE, kpsD, and kpsC genes in region 1 of the K5 capsule gene cluster by subclones of the K10 and K54 capsule gene clusters indicated that certain stages in the export of group II and I/II capsules may be conserved. In the light of the findings presented here, we suggest that the group I/II capsule gene clusters are sufficiently different from group II capsule gene clusters to justify their renaming as group III.

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“…32 This sugar derivative has been reported only once elsewhere as a component of a capsular polysaccharide of Escherichia coli. 33 Serogroup O8 (P. vulgaris PrK 17/57, P. mirabilis TG 326, P. penneri 107, Proteus genomospecies 5)…”

Section: Chemical Structure Of Proteus O-polysaccharidesmentioning

confidence: 99%

Structure and serology of O-antigens as the basis for classification of Proteus strains

et al. 2010

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Structure of the K10 capsular antigen from Escherichia coli O11:K10:H10, a polysaccharide containing 4,6-dideoxy-4-malonylamino-d-glucose

Cited by 10 publications

References 17 publications

Genetic Organization of the Escherichia coli K10 Capsule Gene Cluster: Identification and Characterization of Two Conserved Regions in Group III Capsule Gene Clusters Encoding Polysaccharide Transport Functions

Genetic Organization of the Escherichia coli K10 Capsule Gene Cluster: Identification and Characterization of Two Conserved Regions in Group III Capsule Gene Clusters Encoding Polysaccharide Transport Functions

Cloning and analysis of gene clusters for production of the Escherichia coli K10 and K54 antigens: identification of a new group of serA-linked capsule gene clusters

Structure and serology of O-antigens as the basis for classification of Proteus strains

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