Structure of the O‐polysaccharide and classification of <i>Proteus mirabilis</i> strain G1 in <i>Proteus</i> serogroup O3

Sidorczyk, Zygmunt; Zych, Krystyna; Toukach, Filip V.; Arbatsky, Nikolay P.; Zabłotni, Agnieszka; Shashkov, Alexander S.; Knirel, Yuriy A.

doi:10.1046/j.1432-1033.2002.02782.x

Cited by 56 publications

(51 citation statements)

References 28 publications

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“…The animals were bled one week after the last immunization (Sidorczyk et al 2002c). Serum against whole cells of P. penneri 13 was provided by the Department of General Microbiology, University of £ódŸ.…”

Section: Immunization Procedures and Sources Of The Seramentioning

confidence: 99%

“…Passive immunohemolysis (PIH), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/ /PAGE), Western blot, and enzyme-linked immunosorbent assay (ELISA) were carried out as previously described (Sidorczyk et al 2002c). For PIH, sheep red blood cells (SRBCs) were sensitized with alkali-treated LPSs (64-200 µg/0.2 ml of SRBCs).…”

Section: Serological Assaysmentioning

confidence: 99%

“…The reactivities of twofold serially diluted antisera were tested by PIH. The antiserum titer was established as the last dilution of antiserum resulting in 50% hemolysis (Sidorczyk et al 2002c).…”

Section: Absorption Proceduresmentioning

confidence: 99%

“…To date only a few structural elements have been determined in Proteus LPS core regions as involved in cross-reactions. The first one, D-GalpA6(L-Lys), was present in the core region of P. mirabilis O28 LPS and the other, β-D-Quip4N(LAla), was found in the P. vulgaris TG 103 (O54) and P. mirabilis O6 core oligosaccharides (Ko³odziejska et al 2006;Sidorczyk et al 2002c). Both fragments were identified in the LPS core regions of smooth Proteus sp.…”

Section: Introductionmentioning

confidence: 99%

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Serological characterization of the core region of lipopolysaccharides of rough Proteus sp. strains

Palusiak

Sidorczyk

2009

Arch. Immunol. Ther. Exp.

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Introduction: Both smooth and rough Proteus sp. strains can be found. The latter are characterized by their lack of an O--polysaccharide chain in the lipopolysaccharide (LPS) molecule, which makes them suitable for obtaining anti-core sera. Using this kind of material enables identifying fragments of the Proteus LPS core region that might be involved in cross-reactions. To date only a few similar epitopes have been established for the genus Proteus. Materials and Methods: Polyclonal rabbit antisera directed against three rough strains of Proteus sp. were tested by enzymelinked immunosorbent assay (ELISA) with a set of LPSs. The reactivity of the selected cross-reactive and homologous systems was checked by the Western blot technique and by a passive immunohemolysis assay preceded by the absorption of each antiserum with appropriate cross-reactive and homologous alkalized LPSs. Results: On the basis of the ELISA results, 19 cross-reactive antigens were selected among which both smooth and rough LPS forms were found. All the observed reactions involved the core region of the LPS. Using the antisera absorbed with the appropriate LPSs allowed identification of four groups of antigens with serologically identical core regions. Conclusions:Comparing the results of the serological studies with the known chemical structures of the core regions of the LPSs used enabled the identification of a few core oligosaccharide fragments probably involved in the observed cross-reactions. All were located in the most distal part of LPS core region, which made them more easily recognized by specific antibodies.

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Section: Immunization Procedures and Sources Of The Seramentioning

confidence: 99%

Section: Serological Assaysmentioning

confidence: 99%

Section: Absorption Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Serological characterization of the core region of lipopolysaccharides of rough Proteus sp. strains

Palusiak

Sidorczyk

2009

Arch. Immunol. Ther. Exp.

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“…The results were expressed as percentages of the inhibition of binding. The O polysaccharide from Proteus mirabilis strain G1, a high-molecular-weight polysaccharide containing both positively charged L-lysine (L-Lys) and negatively charged glucoronic acid (GlcA) groups (25) at the same concentrations as PNAG was used as a control in order to exclude the possibility of nonspecific binding. Figure 2 shows a pattern of inhibition of binding of IgG antibodies to PNAG in the sera of guinea pigs infected with S. epidermidis RP62A.…”

Section: Methodsmentioning

confidence: 99%

Potential Use of Poly- N -Acetyl-β-(1,6)-Glucosamine as an Antigen for Diagnosis of Staphylococcal Orthopedic-Prosthesis-Related Infections

Sadovskaya

Faure

Watier

et al. 2007

Clin Vaccine Immunol

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Staphylococcus aureus and coagulase-negative staphylococci are microorganisms most frequently isolated from orthopedic-implant-associated infections. Their capacity to maintain these infections is thought to be related to their ability to form adherent biofilms. Poly-N-acetyl-␤-(1,6)-glucosamine (PNAG) is an important constituent of the extracellular biofilm matrix of staphylococci. In the present study, we explored the possibility of using PNAG as an antigen for detecting antibodies in the blood sera of patients with staphylococcal orthopedic-prosthesis-associated infections. First, we tested the presence of anti-PNAG antibodies in an animal model, in the blood sera of guinea pigs that developed an implant-associated infection caused by biofilm-forming, PNAG-producing strains of Staphylococcus epidermidis. Animals infected with S. epidermidis RP62A showed levels of anti-PNAG immunoglobulin G (IgG) significantly higher than those of the control group. The comparative study of healthy individuals and patients with staphylococcal prosthesis-related infections showed that (i) relatively high levels of anti-PNAG IgG were present in the blood sera of the healthy control group, (ii) the corresponding levels in the infected patients were slightly but not significantly higher, and (iii) only 1 of 10 patients had a level of anti-PNAG IgM significantly higher than that of the control group. In conclusion, the encouraging results obtained in the animal study could not be readily applied for the diagnosis of staphylococcal orthopedic-prosthesis-related infections in humans, and PNAG does not seem to be an appropriate antigen for this purpose. Further studies are necessary to determine whether the developed enzyme-linked immunosorbent assay method could serve as a complementary test in the individual follow-up treatment of such infections caused by PNAG-producing staphylococci.

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Characterization and serological classification of O-specific polysaccharide of Proteus mirabilisTG 276-90 from Proteus serogroup O34

Kołodziejska

Siwińska

Zych

et al. 2006

Arch. Immunol. Ther. Exp.

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Structure of the O‐polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3

Cited by 56 publications

References 28 publications

Serological characterization of the core region of lipopolysaccharides of rough Proteus sp. strains

Serological characterization of the core region of lipopolysaccharides of rough Proteus sp. strains

Potential Use of Poly- N -Acetyl-β-(1,6)-Glucosamine as an Antigen for Diagnosis of Staphylococcal Orthopedic-Prosthesis-Related Infections

Characterization and serological classification of O-specific polysaccharide of Proteus mirabilisTG 276-90 from Proteus serogroup O34

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