Structure of the O‐polysaccharide from Proteus myxofaciens

Abstract

The O‐polysaccharide (O‐antigen) was obtained from the lipopolysaccharide of Proteus myxofaciens, a Proteus strain producing copious amounts of slime, which was isolated from the gypsy moth larvae. The structure of the polysaccharide was studied by chemical analysis and 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, ROESY and H‐detected 1H,13C HMQC experiments. It was found that the polysaccharide contains an amide of glucuronic acid (GlcA) with an unusual α‐linked amino acid, Nε‐[(R)‐1‐carboxyethyl]‐l‐lysine (2S,8R‐alaninolysine, 2S,8R‐AlaLys), and has a linear tetrasaccharide repeating unit of the following structure: This structure is unique among known bacterial polysaccharide structures. On the basis of these and serological data, it is proposed that P. myxofaciens be classified into a new Proteus serogroup, O60, of which this strain is the single representative. Structural and serological relatedness of P. myxofaciens to other AlaLys‐containing O‐antigens of Proteus and Providencia is discussed.

Structure of the O-polysaccharide ofProteus penneri28 andProteus vulgarisO31 and classification ofP. penneri26 and 28 inProteusserogroup O31

“…Polyclonal O‐antisera were obtained by immunisation of rabbits with heat‐inactivated bacteria of P. penneri 26 and 28 and P. vulgaris O31 according to the published procedure [17]. SDS–PAGE (with use of 9.5% acrylamide), immunoblotting, absorption experiments, enzyme immunosorbent assay (EIA) using LPS and passive immunohemolysis test (PI) using alkali‐treated LPS as antigen as well as inhibition experiments were performed as described in detail previously [18].…”

Classification of Proteus mirabilis TG 115 and CCUG 10701 into the Proteus O23 serogroup based on chemical and serological studies of O-polysaccharides

“…Later, new O-serogroups containing strains of other Proteus species were created, i.e. P. penneri [4,16,18,25], P. myxofaciens [15], and the unnamed Proteus genomospecies 4, 5, and 6 [26]. The above scheme is not complete, even for the species P. mirabilis and P. vulgaris, because Larsson et al [5] and Penner and Hennesy [8] supplemented it by 6 and 11 new Proteus O-serogroups, respectively.…”

Structure of the O-polysaccharide and serological studies of the lipopolysaccharide of Proteus mirabilis 2002

“…[5][6][7] A number of further O-serogroups have been proposed for P. penneri [8][9][10][11][12] and P. myxofaciens. 13 In this paper, we report the structure of an acidic O-polysaccharide from the nonclassified strain P. mirabilis 2002 and, based on structural and serological data, propose to classify it as a subgroup of an existing Proteus serogroup, O29. The 1 H and 13 C NMR spectra of the O-polysaccharide were assigned using 2D homonuclear COSY, TOCSY, ROESY, and H-detected 1 H, 13 C HSQC and HMBC experiments ( Table 1).…”

“…13 In this paper, we report the structure of an acidic O-polysaccharide from the nonclassified strain P. mirabilis 2002 and, based on structural and serological data, propose to classify it as a subgroup of an existing Proteus serogroup, O29. The 1 H and 13 C NMR spectra of the O-polysaccharide were assigned using 2D homonuclear COSY, TOCSY, ROESY, and H-detected 1 H, 13 C HSQC and HMBC experiments ( Table 1). The spin systems for Glc and GlcA were identified by correlations of H-1 with H-2 to H-6 and H-2 to H-5, respectively, in the TOCSY spectrum.…”