Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas hydrophila strain K691 containing 4-acetamido-4,6-dideoxy- d -glucose

Pakiet, Katarzyna; Turska-Szewczuk, Anna; Karaś, Magdalena A.; Pękała, Agnieszka; Pietras, Hubert

doi:10.1016/j.carres.2016.12.006

Cited by 7 publications

(3 citation statements)

References 24 publications

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“…It is not clear, but the first published report of its existence was in 1959 from E. coli 30 . Since that time, there have been sporadic reports of the sugar being isolated from the O‐antigens of Hafnia alvei 1205, A. baumannii , Pseudomonas fluorescens , Cellulophaga tyrosinoxydans , and Aeromonas hydrophila 31–36 . An elegant biochemical characterization of the sugar N ‐acetyltransferase from E. coli O7 was described in 2007 37 .…”

Section: Discussionmentioning

confidence: 99%

“…30 Since that time, there have been sporadic reports of the sugar being isolated from the O-antigens of Hafnia alvei 1205, A. baumannii, Pseudomonas fluorescens, Cellulophaga tyrosinoxydans, and Aeromonas hydrophila. [31][32][33][34][35][36] An elegant biochemical characterization of the sugar N-acetyltransferase from E. coli O7 was described in 2007. 37 The kinetic parameters for the E. coli enzyme were similar to those reported here for VioB (K M of 0.14 mM and a k cat /K M of 1.1 Â 10 4 M À1 s À1 ), and interestingly, the Nacetyltransferase from E. coli demonstrated catalytic activity over a temperature range of 4 to 65 C.…”

Section: Production Of Dtdp-4-amino-46-dideoxy-dglucosementioning

confidence: 99%

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Structure and function of an N‐acetyltransferase from the human pathogen Acinetobacter baumannii isolate BAL_212

2022

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Acinetobacter baumannii is a Gram-negative bacterium commonly found in soil and water that can cause human infections of the blood, lungs, and urinary tract. Of particular concern is its prevalence in health-care settings where it can survive on surfaces and shared equipment for extended periods of time. The capsular polysaccharide surrounding the organism is known to be the major contributor to virulence. The structure of the K57 capsular polysaccharide produced by A. baumannii isolate BAL_212 from Vietnam was recently shown to contain the rare sugar 4-acetamido-4,6-dideoxy-D-glucose. Three enzymes are required for its biosynthesis, one of which is encoded by the gene H6W49_RS17300 and referred to as VioB, a putative N-acetyltransferase. Here, we describe a combined structural and functional analysis of VioB. Kinetic analyses show that the enzyme does, indeed, function on dTDP-4-amino-4,6-dideoxy-D-glucose with a catalytic efficiency of 3.9 x 10 4 M À1 s À1 (±6000), albeit at a reduced value compared to similar enzymes. Three high-resolution X-ray structures of various enzyme/ligand complexes were determined to resolutions of 1.65 Å or better. One of these models represents an intermediate analogue of the tetrahedral transition state. Differences between the VioB structure and those determined for the N-acetyltransferases from Campylobacter jejuni (PglD), Caulobacter crescentus (PerB), and Psychrobacter cryohalolentis (Pcryo_0637) are highlighted. Taken together, this investigation sheds new insight into the Type I sugar N-acetyltransferases. K E Y W O R D S 4,6-dideoxyhexoses, 4-acetamido-4,6-dideoxy-D-glucose, 4-amino-4,6-dideoxy-D-glucose, Acinetobacter baumannii, carbohydrate, enzyme structure, N-acetyltransferase 1 | INTRODUCTIONAcinetobacter baumannii is an opportunistic Gram-negative bacterium that has recently emerged as a significant human health threat worldwide. 1,2 Due to the increasing rise of A. baumannii infections in hospital settings, and its extensive antibiotic resistance spectrum, it has been designated as

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Section: Discussionmentioning

confidence: 99%

Section: Production Of Dtdp-4-amino-46-dideoxy-dglucosementioning

confidence: 99%

Structure and function of an N‐acetyltransferase from the human pathogen Acinetobacter baumannii isolate BAL_212

2022

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“…Three or more biochemical tests al- It consists of a double branched heptasaccharide containing D-GalNAc, L-Rha, D-GlcNAc, and D-FucNAc residues. Although these residues have already been found in other O-chains from Aeromonas genus [17][18][19], the structure of the repeating unit was found to be uncommon among the O-chains characterized of species belonging to this genus as well as in other bacterial polysaccharide structures.…”

Section: Bacterial Characteristics Isolation and Growthmentioning

confidence: 85%

Complete Characterization of the O-Antigen from the LPS of Aeromonas bivalvium

Guida

Casillo

Tomás

et al. 2022

IJMS

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Aeromonas species are found in the aquatic environment, drinking water, bottled mineral water, and different types of foods, such as meat, fish, seafood, or vegetables. Some of these species are primary or opportunistic pathogens for invertebrates and vertebrates, including humans. Among the pathogenic factors associated with these species, there are the lipopolysaccharides (LPSs). LPSs are the major components of the external leaflet of Gram-negative bacterial outer membrane. LPS is a glycoconjugate, generally composed of three portions: lipid A, core oligosaccharide, and O-specific polysaccharide or O-antigen. The latter, which may be present (smooth LPS) or not (rough LPS), is the most exposed part of the LPS and is involved in the pathogenicity by protecting infecting bacteria from serum complement killing and phagocytosis. The O-antigen is a polymer of repeating oligosaccharide units with high structural variability, particularly the terminal sugar, that confers the immunological specificity to the O-antigen. In this study, we established the structure of the O-chain repeating unit of the LPS from Aeromonas bivalvium strain 868 ET (=CECT 7113T = LMG 23376T), a mesophilic bacterium isolated from cockles (Cardium sp.) and obtained from a retail market in Barcelona (Spain), whose biosynthesis core LPS cluster does not contain the waaE gene as most of Aeromonas species. After mild acid hydrolysis, the lipid A was removed by centrifugation and the obtained polysaccharide was fully characterized by chemical analysis and NMR spectroscopy. The polymer consists of a heptasaccharide repeating unit containing D-GalNAc, L-Rha, D-GlcNAc, and D-FucNAc residues.

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Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila

et al. 2020

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Structure of the O-specific polysaccharide from the lipopolysaccharide of Aeromonas hydrophila strain K691 containing 4-acetamido-4,6-dideoxy- d -glucose

Cited by 7 publications

References 24 publications

Structure and function of an N‐acetyltransferase from the human pathogen Acinetobacter baumannii isolate BAL_212

Structure and function of an N‐acetyltransferase from the human pathogen Acinetobacter baumannii isolate BAL_212

Complete Characterization of the O-Antigen from the LPS of Aeromonas bivalvium

Acute hypercortisolemia inhibits innate immune parameters in Piaractus mesopotamicus experimentally infected with Aeromonas hydrophila

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