Structure of α1-CB8, a large cyanogen bromide produced fragment from the α1 chain of rat collagen. The nature of a hydroxylamine-sensitive bond and composition of tryptic peptides

Börnstein, Paul

doi:10.1021/bi00814a004

Cited by 143 publications

(61 citation statements)

References 39 publications

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“…3b). At neutral pH neither band was affected (not shown), and there is no Asn-Gly in the sequence for hydroxylamine to cleave (27). This suggests a hydroxyester linkage, possibly fatty acid acylation, to Ser or Thr.…”

Section: Resultsmentioning

confidence: 95%

The γ Subunit Modulates Na+ and K+Affinity of the Renal Na,K-ATPase

Arystarkhova

Wetzel

Asinovski

et al. 1999

Journal of Biological Chemistry

185

186

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The Na؉ ,K ؉ -ATPase catalyzes the active transport of ions. It has two necessary subunits, ␣ and ␤, but in kidney it is also associated with a 7.4-kDa protein, the ␥ subunit. Stable transfection was used to determine the effect of ␥ on Na,K-ATPase properties. When isolated from either kidney or transfected cells, ␣␤␥ had lower affinities for both Na ؉ and K ؉ than ␣␤. A post-translational modification of ␥ selectively eliminated the effect on Na ؉ affinity, suggesting three configurations (␣␤, ␣␤␥, and ␣␤␥*) conferring different stable properties to Na,K-ATPase. In the nephron, segment-specific differences in Na ؉ affinity have been reported that cannot be explained by the known ␣ and ␤ subunit isoforms of Na,K-ATPase. Immunofluorescence was used to detect ␥ in rat renal cortex. Cortical ascending limb and some cortical collecting tubules lacked ␥, correlating with higher Na ؉ affinities in those segments reported in the literature. Selective expression in different segments of the nephron is consistent with a modulatory role for the ␥ subunit in renal physiology.

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Section: Resultsmentioning

confidence: 95%

The γ Subunit Modulates Na+ and K+Affinity of the Renal Na,K-ATPase

Arystarkhova

Wetzel

Asinovski

et al. 1999

Journal of Biological Chemistry

185

186

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“…(1965 The explanation for 1ow recovery may be the instabilíty of the enz)¡me under the purification conditions used in the present study. rn additÍon to low r80 (Bhayana & Duckworth,19g4). rt is evídent from the above composítion calculaËÍons that bacterial (Bornstein, 1970 (Ner et a1., 1983). Most of the overlaps fron protein chemist.ry are establíshed by two or more residues.…”

Section: Cn-10mentioning

confidence: 99%

Amino acid sequence of Escherichia coli citrate synthase

Bhayana¹,

Duckworth²

1984

Biochemistry

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“…The tubes that contained aI(I)CB3 were pooled and dried. Purified al(I)CB3 was cleaved at the single Asn-Gly bond in the peptide with hydroxylamine (25). The sample was dissolved in water and warmed to 40°C, an equal volume ofcold 2 M NH2OH, pH 10.5, was added, and the mixture was incubated at 35°C for 90 min.…”

Section: Determination Ofthermal Stabilitymentioning

confidence: 99%