Structure–Reactivity Studies of Intermediates for Mechanistic Information by Subensemble Fluorescence Microscopy

Evidence for Dynamic Chemical Kinetics at Individual Molecular Ruthenium Catalysts

“…This detection was achieved with catalyst 1 , industrially important norbornene monomer, and a small amount of fluorescent probe 2 as a dopant that enabled detection of polymerization through catalytic turnover as fluorescence intensity increases (Figure ). Using fluorescence microscopy with sensitivity sufficient for the detection of single‐insertion reactions of 2 , the changes in the rate of catalysis with respect to time were detected by changes in the slope of the intensity increase. The incorporation of probe 2 into the growing polymer was chemospecific to ring‐opening metathesis as shown by a control probe containing the identical boron dipyrromethene (BODIPY) core, which lacked a functional group for olefin metathesis and was not incorporated (for details, see the Supporting Information).…”

Evidence for Dynamic Chemical Kinetics at Individual Molecular Ruthenium Catalysts

“…This detection was achieved with catalyst 1 , industrially important norbornene monomer, and a small amount of fluorescent probe 2 as a dopant that enabled detection of polymerization through catalytic turnover as fluorescence intensity increases (Figure ). Using fluorescence microscopy with sensitivity sufficient for the detection of single‐insertion reactions of 2 , the changes in the rate of catalysis with respect to time were detected by changes in the slope of the intensity increase. The incorporation of probe 2 into the growing polymer was chemospecific to ring‐opening metathesis as shown by a control probe containing the identical boron dipyrromethene (BODIPY) core, which lacked a functional group for olefin metathesis and was not incorporated (for details, see the Supporting Information).…”

Optical monitoring of polymerizations in droplets with high temporal dynamic range

“…15,16 Advancements in uorescence microscopy over the last few decades have allowed for remarkable strides to be made in the study of chemical reactions. [17][18][19][20][21][22][23] These techniques can even be employed at the single-molecule level to reveal unsynchronized dynamics of individual catalyst molecules. 19,20,[24][25][26][27][28][29] At the other extreme, the inherent scalability of optical methods makes them attractive readouts for massively parallel, high-throughput combinatorial testing of reaction conditions.…”