2022
DOI: 10.1016/j.fbio.2021.101527
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Structure, retrogradation and digestibility of waxy corn starch modified by a GtfC enzyme from Geobacillus sp. 12AMOR1

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Cited by 11 publications
(9 citation statements)
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“…A strong peak of 45° was found in both samples, but the intensity was higher in jagung bose than in whole corn. Commonly, corn starch's peak at 45° (2θ angle) was not found [36,[41][42][43]. This peak represented higher crystallinity of jagung bose than that of whole corn.…”
Section: Crystallinitymentioning
confidence: 98%
See 1 more Smart Citation
“…A strong peak of 45° was found in both samples, but the intensity was higher in jagung bose than in whole corn. Commonly, corn starch's peak at 45° (2θ angle) was not found [36,[41][42][43]. This peak represented higher crystallinity of jagung bose than that of whole corn.…”
Section: Crystallinitymentioning
confidence: 98%
“…The absorbance at 1047 cm −1 is sensitive to the crystalline amount of starch, and the absorbance at 1022 cm −1 reflects the amorphous starch [36]. The peaks 1022 cm −1 were present for whole corn (1019 cm −1 ) and jagung bose (1021 cm −1 ) with 10 and 17%, respectively.…”
Section: Intensity [Au] Wavenumber [Cm^-1]mentioning
confidence: 99%
“…Given the observed structural similarity in the binding groove between GbGtfC and Lr121 GtfB, it is intriguing that Lr121 GtfB (and other GtfBs) synthesize products with consecutive α-1,6 linkages, whereas GbGtfC forms alternating α-1,4/α-1,6 linkages. In fact, GbGtfC so far is the only biochemically characterized GtfC-type 4,6-α-GT displaying this specificity; understanding this unique property is important regarding its application in starch modification. , We therefore compared the 3D structures of GbGtfC (this study) and Lr121 GtfB and used them to perform molecular docking with donor and acceptor substrates. The active site of GbGtfC seems more constrained around subsites +1/+2 than in Lr121 GtfB (Figure S5a); moreover, while the residues surrounding donor subsites are largely conserved, GbGtfC differs at four positions near acceptor binding subsites (Figure and b).…”
Section: Resultsmentioning
confidence: 99%
“…The decrease in molecular weight of starch was also observed in the reaction catalyzed by the GtfC enzyme. 30 The polydispersity index (PDI) of potato starch increased from 2.65 to 3.87 with an increase in the StGtfB concentration, indicating an increase in the polymer inhomogeneity after StGtfB enzyme modification. The PDI of potato starch decreased gradually from 3.01 to 2.62 with the increase of A1A2 concentration but was still generally higher than 2.50, that of the control group, indicating an increase in the inhomogeneity of polymer after A1A2 enzyme modification.…”
Section: Molecular Dynamics Simulationmentioning
confidence: 98%