2019
DOI: 10.1016/j.bbadis.2019.08.001
|View full text |Cite
|
Sign up to set email alerts
|

Structure-toxicity relationships of saturated and unsaturated free fatty acids for elucidating the lipotoxic effects in human EndoC-βH1 beta-cells

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

6
105
0

Year Published

2019
2019
2024
2024

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 44 publications
(112 citation statements)
references
References 41 publications
6
105
0
Order By: Relevance
“…EndoC-βH1 beta-cells (passages 3-30) and isolated rat islets (from 6 male Lewis rats of 300-400 g) were incubated with different FFA compositions for 24 or 48 h. Thereafter caspase-3 activity 12 and for EndoC-βH1 beta-cells lipid droplet formation 13 , H 2 O 2 generation (see Supplementary Information), as well as RT-PCR for the quantification of ER-stress marker 12 , were measured as described.…”
Section: Experimental Methodsmentioning
confidence: 99%
“…EndoC-βH1 beta-cells (passages 3-30) and isolated rat islets (from 6 male Lewis rats of 300-400 g) were incubated with different FFA compositions for 24 or 48 h. Thereafter caspase-3 activity 12 and for EndoC-βH1 beta-cells lipid droplet formation 13 , H 2 O 2 generation (see Supplementary Information), as well as RT-PCR for the quantification of ER-stress marker 12 , were measured as described.…”
Section: Experimental Methodsmentioning
confidence: 99%
“…The ndings of this study support those of earlier reports [26][27][28] in which EndoC-βH1 cells were shown to be resistant to C16:0 at concentrations of up to 1 mM during exposure periods of 72 h, both in the absence and presence of high glucose concentrations. However, they are at odds with the work of Plötz et al who reported that C16:0, C18:0, C19:0 and C18:1 were toxic to EndoC-βH1 cells [10,29]. Currently, we are uncertain of the reasons for this disparity but it may re ect technical differences since the studies of Plötz et al mainly used caspase 3 activity measurement as a surrogate for apoptosis although they also compared these with other measures of toxicity.…”
Section: Discussionmentioning
confidence: 95%
“…Because the individual fatty acid differently affects its biological activity (Plötz et al, ), we determined the fatty acid compositions of duck and lard oils. As shown in Table , the difference in the fatty acid composition was observed in duck and lard oils.…”
Section: Resultsmentioning
confidence: 99%
“…However, the molecular mechanism(s) underlying the DO‐NE‐mediated attenuation of lipotoxicity remains unclear. A partial explanation for this event is that high ratio of double bonds of duck oil retards the metabolic flux rate and hampers the production of hydrogen peroxide in the β‐oxidation pathways leading to the reduction in hepatic injury (Plötz et al, ). DO‐NE may therefore exert its action on the lipotoxicity through blockade of ROS, which are cellular signaling molecules that regulate gene expression such as PPAR‐γ and SREBP‐1 that directly associated with hepatic lipid accumulation (Eeckhoute et al, ; Ruiz et al, ).…”
Section: Resultsmentioning
confidence: 99%