Structure, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from the subgingival microbiota

Sanchez, Maria Carmen Arroyo; Llama‐Palacios, Arancha; Blanc, Vanessa; León, Rubén; Herrera, David; Sanz, Mariano

doi:10.1111/j.1600-0765.2010.01341.x

Cited by 94 publications

(104 citation statements)

References 33 publications

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“…4). This is consistent with the pioneer character of S. oralis also observed in ex vivo samples from human volunteers (64) and in models of oral biofilms (65). The avidity of S. oralis for human cell surfaces seems to be associated with the activity of specific galactose-or sialic acid/N-acetylgalactosamine-binding lectins (66,67).…”

Section: Discussionsupporting

confidence: 72%

Protective Mechanisms of Respiratory Tract Streptococci against Streptococcus pyogenes Biofilm Formation and Epithelial Cell Infection

Fiedler

Riani

Koczan

et al. 2013

Appl Environ Microbiol

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bStreptococcus pyogenes (group A streptococci [GAS]) encounter many streptococcal species of the physiological microbial biome when entering the upper respiratory tract of humans, leading to the question how GAS interact with these bacteria in order to establish themselves at this anatomic site and initiate infection. Here we show that S. oralis and S. salivarius in direct contact assays inhibit growth of GAS in a strain-specific manner and that S. salivarius, most likely via bacteriocin secretion, also exerts this effect in transwell experiments. Utilizing scanning electron microscopy documentation, we identified the tested strains as potent biofilm producers except for GAS M49. In mixed-species biofilms, S. salivarius dominated the GAS strains, while S. oralis acted as initial colonizer, building the bottom layer in mixed biofilms and thereby allowing even GAS M49 to form substantial biofilms on top. With the exception of S. oralis, artificial saliva reduced single-species biofilms and allowed GAS to dominate in mixed biofilms, although the overall two-layer structure was unchanged. When covered by S. oralis and S. salivarius biofilms, epithelial cells were protected from GAS adherence, internalization, and cytotoxic effects. Apparently, these species can have probiotic effects. The use of Affymetrix array technology to assess HEp-2 cell transcription levels revealed modest changes after exposure to S. oralis and S. salivarius biofilms which could explain some of the protective effects against GAS attack. In summary, our study revealed a protection effect of respiratory tract bacteria against an important airway pathogen and allowed a first in vitro insight into local environmental processes after GAS enter the respiratory tract.

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Section: Discussionsupporting

confidence: 72%

Protective Mechanisms of Respiratory Tract Streptococci against Streptococcus pyogenes Biofilm Formation and Epithelial Cell Infection

Fiedler

Riani

Koczan

et al. 2013

Appl Environ Microbiol

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“…187,188 The inclusion of sheep's blood as a supplement ensured that the broth was rich in protein and haemoglobin products, in order to encourage the growth of facultative and obligate anaerobes. 189 The resulting mixed species biofilm is comparable to that found in an in vivo PI environment established on hydroxyapatite discs. 189…”

Section: Biofilm Modelsupporting

confidence: 51%

Novel methods for debridement of dental implant surfaces contaminated by biofilm

Tran¹

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“…The saliva is collected from healthy adult subjects who have refrained from toothbrushing and other oral hygiene practices for 12 h prior to the collection of stimulated saliva, collected whilst chewing on sterile paraffin wax for 5 min. The incubation times of 72-96 h which we have used in these studies are the same as those used by Sánchez et al [100] in their studies of the growth of pure species using the same BHI growth medium . Their work showed that by 12 h the early colonizers had adhered, the intermediate colonizers appeared at 24 h, the late colonizers were found after 48 h, and the biofilm reached a steady state between 72 and 92 h after initiation.…”

Section: Mixed Biofilm Modelsmentioning

confidence: 82%

“…Using single species models in the laboratory cannot replicate the complexity or the biofilms which form in the clinical situation. Some laboratory studies have developed a mature anaerobic biofilms from multiple strains of known primary, secondary, and tertiary colonizers enriched in a high protein broth [98][99][100]; however, a major limitation in such studies is that the biofilms have been grown on flat hydroxyapatite (HAP) discs.…”

Section: Mixed Biofilm Modelsmentioning

confidence: 99%

Novel Models to Manage Biofilms on Microtextured Dental Implant Surfaces

Tran¹,

Walsh²

2016

Microbial Biofilms - Importance and Applications

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Dental implants are used extensively to replace missing teeth. To enhance their integration with the bones of the jaws, the surfaces of titanium dental implants are modified to make them hydrophilic, high energy, and microtextured. These same features make biofilm development occur readily upon exposure to the saliva. The presence of mature biofilms on dental implant surfaces drives local inflammatory responses in the adjacent soft and hard tissues (peri-implantitis), which leads to pathological loss of bone and the formation of a saucer shaped bone defects. This chapter examines the unique challenges posed by biofilms formed on highly complex dental implant surfaces, which are difficult to access for cleaning, and easily damaged by conventional cleaning approaches. We explore how biofilms can be removed from implant surfaces using a variety of novel methods, without causing surface damage or other undesirable modifications, and show how different laboratory and clinical models can be used to assess the performance of both conventional and novel methods of biofilm removal.

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Structure, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from the subgingival microbiota

Abstract: An in vitro biofilm model was developed and validated, demonstrating a pattern of bacterial colonization and maturation similar to the in vivo development of the subgingival biofilm.

Cited by 94 publications

References 33 publications

Protective Mechanisms of Respiratory Tract Streptococci against Streptococcus pyogenes Biofilm Formation and Epithelial Cell Infection

Protective Mechanisms of Respiratory Tract Streptococci against Streptococcus pyogenes Biofilm Formation and Epithelial Cell Infection

Novel methods for debridement of dental implant surfaces contaminated by biofilm

Novel Models to Manage Biofilms on Microtextured Dental Implant Surfaces

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