2015
DOI: 10.3788/lop52.010003
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Structured Illumination Fluorescence Microscopy: Diffraction-Limit Breaking Principle and Application in Life Science

Abstract: Resolution of traditional fluorescence microscopy is limited by the diffraction of light. Diffraction limit can be broken by structured illumination to get higher resolution. Compared to other super-resolution microscopy techniques, structured illumination fluorescence microscopy can achieve higher imaging speed and need a simple setup, which has an important application life science research. In this paper, we first illustrate the principle and reconstruction algorithm to obtain 2D and 3D super-resolution ima… Show more

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