2012
DOI: 10.1016/s1672-6529(11)60091-7
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Structured PDMS Chambers for Enhanced Human Neuronal Cell Activity on MEA Platforms

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Cited by 26 publications
(23 citation statements)
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“…The 1-well MEA control is an open volume system with no liquid volume restrictions. To analyse the development of network activity, the data were compared to recordings from 60-6wellMEA200/30iR-Ti MEAs (MCS), in which the individual wells were separated using SpikeBooster devices (BioMediTech) (Kreutzer et al, 2012 ), and is referred to as the 6-well MEA control. The dimensions of the cell culturing areas on the SpikeBooster devices are the same as the cell plating areas on the tunnel devices, and the 6-well MEA control can be considered a partially restricted volume system.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The 1-well MEA control is an open volume system with no liquid volume restrictions. To analyse the development of network activity, the data were compared to recordings from 60-6wellMEA200/30iR-Ti MEAs (MCS), in which the individual wells were separated using SpikeBooster devices (BioMediTech) (Kreutzer et al, 2012 ), and is referred to as the 6-well MEA control. The dimensions of the cell culturing areas on the SpikeBooster devices are the same as the cell plating areas on the tunnel devices, and the 6-well MEA control can be considered a partially restricted volume system.…”
Section: Methodsmentioning
confidence: 99%
“…Microengineered polydimethylsiloxane (PDMS) devices can be used to answer specific questions on mechanisms of neural function and pathology (Taylor et al, 2005 ; Scott et al, 2013 ; Ren et al, 2015 ), and they can also be used to facilitate the analysis of electrical function of hPSC-derived networks. PDMS devices consisting an open chamber (or “well”) can guide the network to grow more densely on top of the measuring electrodes, and thus facilitate the development and detection of neuronal activity (Kreutzer et al, 2012 ). PDMS microtunnel devices, on the other hand, increase the detected activity by amplifying extracellular electrical signals detected by the MEA (FitzGerald et al, 2009 ; Wieringa et al, 2010 ; Wang et al, 2012 ).…”
Section: Introductionmentioning
confidence: 99%
“…To analyse the neuronal activity, a single cell suspension was also replated in NDM on 0.05% polyethyleneimine and 20 µg/ml human laminin coated MEA plates ( n  = 8, 6wellMEA200/30iR-Ti, Multi Channel Systems, Germany) containing custom-made poly(dimethylsiloxane, PDMS) culturing chambers (Kreutzer et al, 2012). These chambers have capacity of 200 µl of medium/well and the cell attachment area is limited to 7 mm 2 .…”
Section: Methodsmentioning
confidence: 99%
“…PSCs can be encapsulated in structural materials such as ECM [75] or in spatially organized, 3D hydrogels that incorporated signaling factors [76]. Microwells have even been developed in conjunction with microelectrode arrays that can be used to characterize electrical activity of cells [77]. Microwells have even been designed so that the bottom face is a fabricated electrospun network of nano/microfibers [78].…”
Section: Conclusion and Future Perspectivesmentioning
confidence: 99%