Structures of trichovirins II, peptaibol antibiotics from the moldTrichoderma viride NRRL 5243

Jaworski, Andreas; Kirschbaum, Jochen; Brückner, Hans

doi:10.1002/(sici)1099-1387(199908)5:8<341::aid-psc204>3.0.co;2-0

Cited by 34 publications

(31 citation statements)

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“…cucumerinum. Jaworski et al (1999) isolated a mixture of polypeptides, named trichovirins (TV) from the culture broth of the mold T. viride NRRL 5243. Also Krause et al (2005) isolated polypeptide antibiotic suzukacillin (SZ) from the culture broth of the mold T. viride, strain 63 C-I.…”

Section: Control Of Pathogen By Coating Plant Stems With Antagonists mentioning

confidence: 99%

Fungal Control of Pathogenic Fungi Isolated From Some Wild Plants in Taif Governorate, Saudi Arabia

.M.¹,

Altalhi²,

I.³

2008

MJM

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(G. deliquescens, G. virens, T. viride and T. hamatum) significantly inhibited the radial growth of the pathogenic fungi tested, with different ratios. The results indicated that the antibiotics produced by the antagonists were more effective than the fungus itself and differ with different fungi. Coating plant stems with antagonists or with antagonist extracts reduce the severity of the disease but not prevent it in all tested pathogens.

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Section: Control Of Pathogen By Coating Plant Stems With Antagonists mentioning

confidence: 99%

Fungal Control of Pathogenic Fungi Isolated From Some Wild Plants in Taif Governorate, Saudi Arabia

.M.¹,

Altalhi²,

I.³

2008

MJM

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“…Peptide fragment-ion series were assigned in accordance with the Roepstorff/Fohlman -Biemann nomenclature previously used [67] [68].…”

Section: Experimental Partmentioning

confidence: 99%

“…The isobaric sequence with Iva 8 instead of Val 8 has not yet been published. The C-terminal motif, Aib-Pro-Leu-Aib-Aib-Gln-Leuol, comprising positions 12 -18, has been described for the following 18-residue peptaibols, namely trichokindins IIb, IIIa, IIIb, and VI from a strain classified as T. harzianum [43], trichorzins HA I and HA III from T. harzianum M 903602 and M 922835 [44] [45], for hypomurocins B II and B V from T. atroviride, formerly described as Hypocrea muroiana IFO 31288 [46], and for trichovirins Ia, Ib, IIa, IIb, IIc, IIIa, IVa, IVb, V, and VIb from T. viride NRRL 5243 [47]. The strain T. viride NRRL 5243 is currently deposited as T. cf.…”

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Peptaibiomics: Screening for Polypeptide Antibiotics (Peptaibiotics) from Plant-ProtectiveTrichoderma Species

Degenkolb¹,

Gräfenhan²,

Berg³

et al. 2006

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were selected because of their antagonistic potential against Eutypa dieback and Esca which are fungal diseases of grapevine trunks. These isolates were screened for the production of a group of polypeptide antibiotics named peptaibiotics, including its subgroups peptaibols and lipopeptaibols. Fully-grown fungal cultures on potato-dextrose agar were extracted with CH 2 Cl 2 /MeOH, and these extracts were subjected to SPE using C 18 cartridges. The methanolic eluates were analyzed by on-line LC/ESI-MS n coupling -a method which is referred to as peptaibiomics . New seven-, ten-, and eleven-residue lipopeptaibols, with N-terminal alkanoyl, and Cterminal leucinol or isoleucinol residues were found and named lipostrigocins and lipopubescins. Furthermore, new 18-residue peptaibols named trichostromaticins and 19-residue peptaibols named trichostrigocins were discovered. One peptaibiotic carrying a free C-terminal valine (or isovaline) named trichocompactin XII was also sequenced. These results corroborate the hypothesis that peptaibiotics might contribute to the plant-protective action of their fungal producers. The data also point out that comparison of peptaibiotic sequences is of limited relevance in order to establish chemotaxonomic relationships among species of the genus Trichoderma.

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“…Lower bacterial biomass in the cultures with protozoa must be attributable to protozoan grazing 1,6,11,14,26) . Those with fungi may be attributable to competition for glucose with the bacterium or antibacterial agent(s) excreted by T. viride 8,9,13) . The biomass of live protozoan cells, live fungal cells, and the amount of protozoan excreta or DOC were also apparently constant, irrespective of the culture mixture (one-way ANOVA, P<0.05) (Fig.…”

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Respiration and Carbon Balance of the Bacterium Pseudomonas sp., a Protozoan Tetrahymena thermophila, and a Fungus Trichoderma viride in a Food Chain System with Glass Beads

2008

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The respiration rates per unit of microbial biomass (qCO2) of live cells were evaluated in inorganic medium using glass beads to which glucose was added at a rate of 50 µg C vial −1 day −1 . The qCO2 of live cells (g CO2-C (g biomass-C) −1 day −1 ) was 0.23 in a pure culture of bacteria, 0.67 in a mixed culture of bacteria and protozoa, 0.16 in a mixed culture of bacteria and fungi, and 0.20 in a mixed culture of bacteria, protozoa and fungi. A culture system containing glass beads is a useful tool to estimate qCO2 of live cells.Key words: microbial food chain, cell-specific respiration, carbon balance, grazing, microcosmThe rate of CO 2 evolution per unit of microbial biomass (metabolic quotient, qCO2) in soil is an indispensable factor for the evaluation of microbial activity 7,29) , the maintenance requirement of microorganisms 2) , or turnover rate of microbial biomass 19) . The structure of the microbial food chain system may be one of the factors affecting qCO2. In our previous study 26) , for example, qCO 2 in a food chain system consisting of glucose, bacteria and protozoa was 13-fold higher than in the system of glucose and bacteria without the protozoa. This difference was attributable to the fact that microbial biomass in the presence of protozoa was one 12th of that in their absence, but the rate of CO2 evolution from the system was almost the same, irrespective of the presence of protozoa. Microbial food chain systems in soil contain fungi as well as bacteria and protozoa. Thus, investigation of qCO2 in the presence of fungi is also needed.Microbial biomass in soil has been estimated either by fumigation-extraction or incubation methods 27,28) . These methods are unable to distinguish live from dead cells. Although direct microscopic observation was sometimes applied 18,21,26) , live cells were not distinguished from dead cells; therefore, the qCO2 of live cells has not yet been evaluated.In this study, the qCO 2 of live cells was estimated in pure cultures of bacteria or mixed cultures with protozoa and/or fungi. Live cells were distinguished from dead ones by a staining method used with direct microscopic observation; however, studies on microbes in soil using direct microscopic observation are very laborious and difficult 5) because soil contains a lot of organic and inorganic particles which are similar in size and shape to microorganisms and have autofluorescence. We therefore used glass beads as model soil particles in this study because they are uniform in shape and contain neither debris nor organic compounds.Pseudomonas sp. strain DP-4 24) was used as a model heterotrophic bacterium. It was preincubated in an inorganic medium 26) supplemented with 1.6 mg C mL −1 of glucose for 6 days, with shaking, at 30°C and the bacterial cells were then collected by centrifugation (10,000×g, 15 min.). Tetrahymena thermophila inbred strain B, provided by Dr. T. Sugai of Ibaraki University, was used as a model protozoan. It was preincubated in a PYG medium (Bacto TM Peptone [Becton, Dickinson and Company, ...

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Structures of trichovirins II, peptaibol antibiotics from the moldTrichoderma viride NRRL 5243

Cited by 34 publications

References 30 publications

Fungal Control of Pathogenic Fungi Isolated From Some Wild Plants in Taif Governorate, Saudi Arabia

Fungal Control of Pathogenic Fungi Isolated From Some Wild Plants in Taif Governorate, Saudi Arabia

Peptaibiomics: Screening for Polypeptide Antibiotics (Peptaibiotics) from Plant-ProtectiveTrichoderma Species

Respiration and Carbon Balance of the Bacterium Pseudomonas sp., a Protozoan Tetrahymena thermophila, and a Fungus Trichoderma viride in a Food Chain System with Glass Beads

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