Strut size and surface area effects on long-term in vivo degradation in computer designed poly(l-lactic acid) three-dimensional porous scaffolds

Saito, Eiji; Liu, Yifei; Migneco, Francesco; Hollister, Scott J.

doi:10.1016/j.actbio.2012.03.028

Cited by 46 publications

(26 citation statements)

References 62 publications

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“…An adequate mechanical strength and appropriate degradation rates are critically important parameters for porous scaffolds used for tissue engineering . The compressive moduli and degradation rates of scaffolds depend on several factors, including the nature of the material, the molecular weight of the polymer, and the pore architecture of the scaffold . Similar to previous studies, our results showed that the mechanical properties of the PDLLA samples decrease when the porosity increases and/or the diameter size of the porogen decreases .…”

Section: Discussionsupporting

confidence: 87%

“…This could be attributed to both a surface area effect and a wall effect because scaffolds with lower porosities and/or bigger pores demonstrate smaller surface areas and thicker pore walls. Therefore, the diffusion of the acidic degradation by‐products out from the samples decreases and, in turn, induces a stronger acid‐catalyzed hydrolysis of PDLLA . In addition, the PL scaffolds possess higher interconnected pores than the SC monolith, and thus facilitates the removal of the acidic by‐products …”

Section: Discussionmentioning

confidence: 99%

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Scaffolds for epithelial tissue engineering customized in elastomeric molds

et al. 2017

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Restoration of soft tissue defects remains a challenge for surgical reconstruction. In this study, we introduce a new approach to fabricate poly(d,l-lactic acid) (PDLLA) scaffolds with anatomical shapes customized to regenerate three-dimensional soft tissue defects. Highly concentrated polymer/salt mixtures were molded in flexible polyether molds. Microcomputed tomography showed that with this approach it was possible to produce scaffolds with clinically acceptable volume ratio maintenance (>90%). Moreover, this technique allowed us to customize the average pore size and pore interconnectivity of the scaffolds by using variations of salt particle size. In addition, this study demonstrated that with the increasing porosity and/or the decreasing of the average pore size of the PDLLA scaffolds, their mechanical properties decrease and they degrade more slowly. Cell culture results showed that PDLLA scaffolds with an average pore size of 100 µm enhance the viability and proliferation rates of human gingival epithelial cells up to 21 days. The simple method proposed in this article can be extended to fabricate porous scaffolds with customizable anatomical shapes and optimal pore structure for epithelial tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 880-890, 2018.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Scaffolds for epithelial tissue engineering customized in elastomeric molds

et al. 2017

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“…Many authors have studied PLLA degradation, in relation to such aspects as pH, crystallinity, and the % of absorbed water, among others . However, there are relatively few results on the effects of nanoparticles such as nanohydroxyapatite (nHA) on PLLA aging and degradation behavior .…”

Section: Introductionmentioning

confidence: 99%

In vitro degradation of PLLA/nHA composite scaffolds

et al. 2013

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Porous Poly‐l‐lactide (PLLA) scaffolds and PLLA/nanohydroxyapatite (nHA) composite scaffolds with interconnected pore networks and a porosity of over 90% were fabricated with lyophilization techniques. In this study, the degradation behavior of PLLA and PLLA/nHA composite scaffolds is investigated over 8 weeks in phosphate buffer solution at 37°C. Thermal analysis using differential scanning calorimetry (DSC) showed that the percent crystallinity of all the samples increased by approximately 10%, which represents a considerable increase in the glass transition temperature. The melting range enthalpy of the scaffolds did not change to lower temperatures as would be expected. The spectroscopic analysis performed by Fourier transform infrared spectroscopy suggested that nHA particles should not appreciably affect the absorbance pattern when evenly mixed with the PLLA. This is consistent with the analysis of the scaffold microstructure and morphology with scanning electron microscopy, which drew a low content of nHA with no significant effect on solvent crystallization or pore structure. The compressive modulus and the yield strength of the scaffolds were investigated in conjunction with the study of their degradation rates. In comparison with the mechanical properties of the PLLA scaffolds, which remained largely unchanged, those of the PLLA/nHA composite scaffolds decreased as the degradation progressed. POLYM. ENG. SCI., 54:2571–2578, 2014. © 2013 Society of Plastics Engineers

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“…Models focusing on the carriers focus on the mechanical [28,[45][46][47], chemical [48][49][50][51] and/or morphological aspects [52][53][54][55] of the carrier with the aim of understanding its influence on the behaviour of the seeded cells and subsequently optimizing its design. Smeets et al [28] use an individual cell based model to investigate the influence of the stiffness of microcarriers on the proliferation of the cells seeded onto it ( figure 1, cell level).…”

Section: Application To Regenerative Orthopaedicsmentioning

confidence: 99%

Regenerative orthopaedics: in vitro, in vivo … in silico

Geris

2014

International Orthopaedics (SICOT)

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In silico, defined in analogy to in vitro and in vivo as those studies that are performed on a computer, is an essential step in problem solving and product development in classical engineering fields. The use of in silico models is now slowly easing its way into medicine. In silico models are already used in orthopaedics for the planning of complicated surgeries, personalised implant design and the analysis of gait measurements. However, these in silico models often lack the simulation of the response of the biological system over time. In silico models focusing on the response of the biological systems are in full development. This review starts with an introduction into in silico models of orthopaedic processes. Special attention is paid to the classification of models according to their spatiotemporal scale (gene/protein to population) and the information they were built on (data vs hypotheses). Subsequently, the review focusses on the in silico models used in regenerative orthopaedics research. Contributions of in silico models to an enhanced understanding and optimisation of four key elements -cells, carriers, culture and clinics -are illustrated. Finally, a number of challenges are identified, related to the computational aspects but also to the integration of in silico tools in clinical practice.

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Strut size and surface area effects on long-term in vivo degradation in computer designed poly(l-lactic acid) three-dimensional porous scaffolds

Cited by 46 publications

References 62 publications

Scaffolds for epithelial tissue engineering customized in elastomeric molds

Scaffolds for epithelial tissue engineering customized in elastomeric molds

In vitro degradation of PLLA/nHA composite scaffolds

Regenerative orthopaedics: in vitro, in vivo … in silico

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