Studien zur quantitativen Serumproperdinbestimmung

Determination of Serum Properdin Levels by Immunodiffusion

“…Radioimmunodiffusion, as described by R owe [10], would probably be well suited for our problem, but is too timeconsuming for large scale use. Methods based on the chemical or immu nochemical analysis of proteins absorbed to zymosan or inulin [3,7] are theoretically unacceptable, as pure properdin forms only a small part of the serum proteins that bind to these polysaccarides. In the study of P ensky et al [6], the activity per optical density unit of properdin elut ed from zymosan is approximately 16°/o of the activity of pure proper din.…”

“…Other methods [3,7] based on the chemical analysis of proteins bound in vitro to zymosan or inulin have also been described. However, Pensky et al [6] demon strated that other proteins as well as properdin are bound to zymosan, thus making the interpretation of results obtained by these methods un certain.…”