Studies for IAA (Indole-3-Acetic Acid) Production by Isolates H6 with Nitric Acid Mutation

Abstract

Nitric acid mutations are known could be used for strain improvement. This research aimed to study Indole-3-Acetic Acid (IAA) production by nitric acid mutan were compared with wild type. Mutation were conducted with some different treatment time such as 0, 30, 60, 90, and 120 min subsequently it were measured for IAA production. Isolate H6 as wild type isolates were also molecularly identified. The wild strain exhibited 53.83 µg ISSN 1978-3477, eISSN 2087 *Corresponding author: Phone: +62-21-5663232 ext. 8765, Fax:+62-21-5602575; Email: raha009@lipi.go.id regulation and nutritional balance, resistance induction for against plant pathogens, solubilizing nutrients for easy uptake by plants also synergistic and antagonistic interactions with microorganisms within the rhizosphere and beyond in bulk soil, which indirectly boosts plant growth rate (Vejan et al. 2016). Production of indole-3-acetic acid (IAA) hormone are widespread in a major property of rhizosphere bacteria that could stimulate and facilitate the plant growth (Glick and Pattern 1996;Damam et al. 2016). IAA function is to promote the growth by several ways in plant. IAA are known promote growth of auxiliary bud and bud formation, help in the apical dominance, stimulate lateral and adventitious root development and growth. Besides development, IAA were also play crucial role in leaf and flower abscission (Kundan et al. 2015).Genus of PGPR could produce IAA by several pathway. Biosynthesis of IAA which are used by single bacterial strain sometimes containing more than one pathway (Patten and Glick 1996). The most widely pathway of IAA biosynthetic in bacteria is indole-3-pyruvate (IPA) and indole-3-acetamide (IAM) anabolic pathways. Those pathways used tryptophan as Plant growth promoting rhizobacteria (PGPR) are a group of microorganisms which inhabiting around or on the root surface. They are known for plant growth promotion and development,both directly or indirectly by producing and secreting of various regulatory chemicals in the vicinity of rhizosphere (Ahemad and Kibret 2014). PGPR could be used as inoculants of biofertilizers in agriculture (Daman et al. 2016). The genera of PGPR include Azoarcus, Azobacter, Azorhizobium, Azospirillum, Azotobacter, Bacillus, Beijerinckia, Burkholderia, Chryseobacterium, Frankia, Gluconacetobacter, Herbaspirillum, Mycobacterium, Paenibacillus, Phyllobacterium, P s Sphingomonas, and Streptomyces (Vejan et al. 2016). In addition, genus Acetobacter, Enterobcter, Actinobacteria, Brevibacillus, Lysinibacillus, Terribacillus and Jeotgalibacillus also included PGPR (Kundan et al. 2015). e u d o m o n a s , R h i z o b i u m , S i n o rh i z o b i u m ,The mechanisms of PGPR include hormonal

Isolation and Characterization of Iaa-Producing Rizosphere Bacteria From Rice Plants (Oryza Sativa L.) in Kedungpani Urban Village, Semarang City

“…In the tryptophan dependent pathway, there are five known pathways that use tryptophan as a precursor, namely indole-3-acetamide (IAM), indole-3pyruvate (IpyA), tryptamine (TAM), Trp side ring oxidase and the indole-3acetonitrile pathway (IAN) (Szkop & Bielawski, 2013). Most bacteria carry out IAA biosynthesis used the indole-3pyruvate (IpyA) and indole-3-acetamide (IAM) pathways (Putrie et al, 2017).…”