Studies <i>in vitro</i> on the uptake and degradation of sodium hyaluronate in rat liver endothelial cells

Smedsrød, Bård; Pertoft, Håkan; Eriksson, Sigbritt; Fraser, J. R. E.; Laurent, Torvard C.

doi:10.1042/bj2230617

Cited by 294 publications

(160 citation statements)

References 25 publications

Supporting

Mentioning

151

Contrasting

Order By: Relevance

“…Reduced elimination of HA may contribute to increased serum HA levels. The endothelial cells of the liver sinusoids play a central role in the degradation of HA, and very high serum HA levels have been demonstrated in patients with cirrhosis of the liver (24)(25)(26). However, since none of the patients in the present study had impaired liver or kidney function, it is unlikely that reduced elimination could explain the elevated serum concentrations of HA.…”

Section: Discussionmentioning

confidence: 60%

Serum hyaluronate level as a predictor of radiologic progression in early rheumatoid arthritis

Paimela

Heiskanen

Kurki

et al. 1991

Arthritis & Rheumatism

View full text Add to dashboard Cite

PATIENTS AND METHODSRA patients. Forty consecutive patients (31 women, 9 men) with newly diagnosed definite or classic RA (according to the 1958 criteria of the American Rheumatism Association [9]) were included in the study. The mean duration of disease in the RA patients at the beginning of the study was 7.0 months (range 2-12 months). The mean age of the patients was 41.5 years (range 19-61). At the time of diagnosis, 27 of the patients (67.5%, 18 women and 9 men) were

show abstract

Section: Discussionmentioning

confidence: 60%

Serum hyaluronate level as a predictor of radiologic progression in early rheumatoid arthritis

Paimela

Heiskanen

Kurki

et al. 1991

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…11 In the liver, the sinusoidal liver endothelial cells (LECs) are the most important sites of hepatic extraction of HA, with the Kupffer cells playing a relatively minor role in HA clearance under normal conditions. [14][15][16][17] The removal of HA from the blood is very efficient, resulting in a half-life of less than 1 minute (in rats) 18 and is facilitated by endocytic cell surface receptors on LECs, which also recognize chondroitin sulfate (CS). 15,16 Formaldehydetreated albumin (FSA), a scavenger receptor (SR) ligand, blocks the uptake of CS, 19 suggesting that the HA/CS receptor is functionally related to the family of SRs.…”

mentioning

confidence: 99%

“…[14][15][16][17] The removal of HA from the blood is very efficient, resulting in a half-life of less than 1 minute (in rats) 18 and is facilitated by endocytic cell surface receptors on LECs, which also recognize chondroitin sulfate (CS). 15,16 Formaldehydetreated albumin (FSA), a scavenger receptor (SR) ligand, blocks the uptake of CS, 19 suggesting that the HA/CS receptor is functionally related to the family of SRs. The affinity of the receptor(s) for HA is dependent on the size of the latter: for example, the K d values for HA octasaccharides and HA of M r 6.4 ϫ 10 6 are 1.4 µmol/L and 9 pmol/L, respectively.…”

mentioning

confidence: 99%

Characterization of a hyaluronan receptor on rat sinusoidal liver endothelial cells and its functional relationship to scavenger receptors

McCourt¹,

Smedsrød²,

Melkko³

et al. 1999

Hepatology

130

151

View full text Add to dashboard Cite

(1-4) and ␤(1-3) glycosidic bonds, respectively, belies the range of unique viscoelastic and physiological properties that this anionic polysaccharide has. Such qualities are probably conferred both by its high molecular mass and its secondary and tertiary structures that include 2-fold helices and extensive branched networks 2 and possibly sheets and tubular structures. 3 The formation of these secondary and tertiary structures is likely facilitated by both hydrophobic and hydrophilic interactions between HA monomers. 3

show abstract

“…It is now well established that the population of LEC in mammals serves as the most important site of elimination of an array of circulating soluble macromolecular waste products. To carry out this scavenger function, LEC express at least five types of specific receptors for endocytosis of major physiologic waste products: (i) the hyaluronan receptor for major matrix polysaccharides and proteoglycans such as hyaluronan and chondroitin sulfate (8); (ii) the collagen alpha-chain receptor for collagen alpha-chains of several types of collagen (9); (iii) the scavenger receptor for amino-terminal propeptides of types I and III procollagen (4), modified macromolecules such as atherogenic advanced glycation end (AGE) products (5) and oxidized LDL (6), oligodeoxynucleotides (10), and blood proteins modified by the process of blood clotting and platelet activation (11); (iv) the mannose receptor for carboxy-terminal propeptide of type I procollagen (7) and tissue plasminogen activator (12); and (v) the Fc-␥ receptor for IgG-antigen immune complexes (13). It is interesting to note that the LEC are able to clear all major categories of biological macromolecules (proteins, polysaccharides, lipids, and nucleic acids) by means of these five types of endocytosis receptors.…”

mentioning

confidence: 99%

Scavenger endothelial cells of vertebrates: A nonperipheral leukocyte system for high-capacity elimination of waste macromolecules

Seternes¹,

Sørensen²,

Smedsrød³

2002

Proc. Natl. Acad. Sci. U.S.A.

140

131

View full text Add to dashboard Cite

I n his pioneering work on the ultrastructure of the hepatic sinusoid 30 years ago, Wisse (1) observed numerous ''smoothwalled macropinocytic vesicles'' in the sinusoidal liver endothelial cells (LEC), suggestive of active pinocytic scavenging of blood plasma proteins. The subsequent finding that the specific activities of lysosomal enzymes in LEC were as high as in the Kupffer cells (2), and for some enzymes even higher, substantiated Wisse's notion. In a series of papers over the next 20 years, it was shown that LEC eliminate an array of soluble macromolecular physiologic and foreign waste products from the circulation by receptor-mediated endocytosis (3-7). It is now well established that the population of LEC in mammals serves as the most important site of elimination of an array of circulating soluble macromolecular waste products. To carry out this scavenger function, LEC express at least five types of specific receptors for endocytosis of major physiologic waste products: (i) the hyaluronan receptor for major matrix polysaccharides and proteoglycans such as hyaluronan and chondroitin sulfate (8); (ii) the collagen alpha-chain receptor for collagen alpha-chains of several types of collagen (9); (iii) the scavenger receptor for amino-terminal propeptides of types I and III procollagen (4), modified macromolecules such as atherogenic advanced glycation end (AGE) products (5) and oxidized LDL (6), oligodeoxynucleotides (10), and blood proteins modified by the process of blood clotting and platelet activation (11); (iv) the mannose receptor for carboxy-terminal propeptide of type I procollagen (7) and tissue plasminogen activator (12); and (v) the Fc-␥ receptor for IgG-antigen immune complexes (13). It is interesting to note that the LEC are able to clear all major categories of biological macromolecules (proteins, polysaccharides, lipids, and nucleic acids) by means of these five types of endocytosis receptors. The blood concentration of most of these substances is normally very low (ng͞ml) because of highly efficient elimination by endocytosis receptors on LEC.On the basis of several studies showing that LEC represent an important nonphagocytic scavenger cell in mammals, it has been suggested that the hepatic scavenger function is shared between Kupffer cells, which eliminate insoluble waste by phagocytosis, and LEC, which remove soluble colloidal or macromolecular waste (3). Hypothesizing that the scavenger function of LEC in mammals be represented by similar nonmacrophagic, nonphagocytic scavenger endothelia in animals of all vertebrate classes, we set out to screen animal species from the seven major vertebrate classes for the presence of scavenger cells equivalent of mammalian LEC.The findings from these studies strongly suggest the presence of a nonmacrophagic system of scavenger endothelial cells that clear the blood from an array of circulating waste macromolecules and colloids. These scavenger endothelial cells make up the backbone of the reticuloendothelial system (RES), and thus represent an import...

show abstract

Studies in vitro on the uptake and degradation of sodium hyaluronate in rat liver endothelial cells

Cited by 294 publications

References 25 publications

Serum hyaluronate level as a predictor of radiologic progression in early rheumatoid arthritis

Serum hyaluronate level as a predictor of radiologic progression in early rheumatoid arthritis

Characterization of a hyaluronan receptor on rat sinusoidal liver endothelial cells and its functional relationship to scavenger receptors

Scavenger endothelial cells of vertebrates: A nonperipheral leukocyte system for high-capacity elimination of waste macromolecules

Contact Info

Product

Resources

About