Studies in Blood Preservation

Scudder, John; Drew, Charles R.; Corcoran, Dorothy R.; Bull, David C.

doi:10.1001/jama.1939.02800220029009

Cited by 18 publications

(3 citation statements)

References 46 publications

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“…The concentration of this cation in the red cells is about twenty times as great as in the plasma. During storage, however, there is a loss of potassium from the red cells, (Duliere, 1931a(Duliere, , 1931bJeannei^y et al, 1938aJeannei^y et al, , 1938bDeGowin et al, 1939DeGowin et al, , 1940Scudder et al, 1939Scudder et al, , 1940Scudder et al, , 1941Denstedt, 1941;Muether andAndrews, 1941a, 1941b;Loutit, 1943Loutit, -1944Maizels, 1943Maizels, -1944 and a number of others. )…”

Section: Methodsmentioning

confidence: 99%

Chemical changes in stored blood

1969

Cryobiology

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Section: Methodsmentioning

confidence: 99%

Chemical changes in stored blood

1969

Cryobiology

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“…Another possible danger from the transfusion of blood which has been stored too long has been the high potassium content of the plasma (Scudder, Drew, Corcoran, and Bull, 1939). It is true that during storage some of the potassium of the erythrocytes diffuses into the plasma, but studies in our laboratory (DeGowin, Harris, and Plass, 1940b) have shown that the potassium concentration in plasma approaches a maximum in fifteen days of storage, and transfusion of blood stored for this period has produced no clinical reactions, no increase in the serum potassium of the recipients, and no electrocardiographic changes (DeGowin, Hardin, and Harris, 1940).…”

Section: Period Of Storagementioning

confidence: 99%

Reactions from Transfusion of Preserved Blood. Experience with 1,600 Transfusions

DeGowin¹,

Hardin²

1940

BMJ

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The convenience of using preserved blood for transfusions was first demonstrated by Robertson (1918), working at the casualty clearing stations of the Third Army, B.E.F., in 1917-18. He collected and stored blood during periods of inaction at the front and administered it during rLush times, utilizing the blood-dextrose-citrate mixture which had been devised by Rous and Turner (1916) if found to be acid, sodium bicarbonate was administered orally until the urine was alkaline before the transfusion was performed. However, this procedure was not practicable in emergencies. Within twenty-four hours after transfusion a member of the laboratory staff visited the patient to determine whether a transfusion reaction had occurred, and performed chemical tests for haemoglobin on the twenty-four-hour urine specimen passed after transfusion. If there had been a severe reaction of any type, tests for groups and cross-matching were repeated. Collection of BloodThe donors who were accepted were healthy adults of either sex between the ages of 16 and 60. They were questioned specifically for a history of venereal disease, malaria, and allergic manifestations. They were not allowed to give blood if suffering from the slightest upper respiratory infection. This precaution was employed so that the subsequent course of such a disease could not be. ascribed to loss of blood. No donor was accepted who was suffering from active manifestations of allergy, although the blood of persons having hay-fever was used when drawn during the months when they were free from symptoms. At least three hours must have elapsed since the donor had eaten before the blood was drawn. No donor was allowed to give more than 500 c.cm. of blood in two months.The collecting apparatus consisted of an Erlenmeyer flask of 1,500 c.cm. capacity in the mouth of which was a twoholed rubber stopper. Short glass tubes were fitted into the holes of the stopper. The outer end of one glass tube was attached to a length of rubber tubing connected to a rubber suction-bulb containing a valve. The other glass tlbe was connected by means of a short gum-rubber tube to a glass adapter which fitted into a needle. After the apparatus was assembled the sterile solution of sodium citrate was aspirated through the needle and rubber tubing and into the flask, and the tubing was pinched off until the needle was inserted in the vein. This minimized the tendency to clotting which occasionally occurred in the collecting-tube. The flask was calibrated with a scale on the side so that the volume of blood mixture could be measured. When the dextrose-citrate mixture was used the dextrose solution was autoclaved in the collecting-flask.The donor assumed a supine position on an examining table with the arm supported in abduction by an arm rest. The antecubital fossa was cleansed first with tincture of iodine and 4148

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“…There is no reason to attribute any significant change in plasma K to the effect of storage or ageing a t -195°C. Indeed, the results for ox blood indicate, with passage of time in the solid state, as in the liquid, an increasing reversal on addition of glucose to mixture I1 ( 1 2 ) , of the initially rapid migration of potassium from cells to plasma ( 13), in this case offsetting an increasing amount released from hemolyzed cells.…”

mentioning

confidence: 97%