Studies in fat metabolism

Jowett, Maurice; Quastel, J. H.

doi:10.1042/bj0292159

Cited by 76 publications

(10 citation statements)

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“…The slice technique made possible precise quantitative measurements not attainable by thc old perfusion methods. We (23) were able to show that acetone bodies could not wholly arise from butyric acid formed during fatty acid oxidation by a process of P-oxidation, as was previously thought, since octanoic acid and hexanoic acid gave rise to larger yields of acetone bodies than those produced by butyric acid; furthermore, more than one molecule of acetoacetate was formed from a molecule of a long-chain fatty acid. Odd-numbered fatty acids could give rise to acetone bodies, and P-hydroxybutyrate and acetate were not necessary intermediates in the formation of acetoacetate from the fatty acids.…”

Section: Cardiffsupporting

confidence: 56%

Fifty Years of Biochemistry. A Personal Account

Quastel¹

1974

Can. J. Biochem.

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Section: Cardiffsupporting

confidence: 56%

Fifty Years of Biochemistry. A Personal Account

Quastel¹

1974

Can. J. Biochem.

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“…These "ketone bodies" were presumed to arise from the last four carbon atoms of the chain. Earlier work on isolated tissues by Quastel 19 , Edson 20 , Stadie 21 and others produced much confirmatory evidence in support of /?-oxidation and added the new observation that, contrary to the older assumption, more than one molecule of ketone body could be formed from one fatty acid molecule. It is further of interest to record that inhibitor experiments with malonate by Quastel 23 reported independently that acetoacetate could cause a formation of citrate in animal tissues if oxaloacetate was present.…”

Section: Early Workmentioning

confidence: 97%

The Tricarboxylic acid cycle (revision number 25)

Ward¹

2015

Diapedia

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“…Rev sponding keto acid, i.e., oxaloa.cetic add, are : desaturation, hydration, and dehydrogenation. As is well kl�own, butyric, crotonic, and hy droxybutyric acids are easily oxidized to acetoacetic acid (147). Therefore, a mechanism analogous to that for succinic acid has been suggested in spite of evidence to the contrary.…”

Section: Fatty Acid Oxidationmentioning

confidence: 99%

Biological Oxidations and Reductions

Lipmann¹

1943

Annu. Rev. Biochem.

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Correlation between metabolism and phosphate turnover.Through a subtle analysis of the metabolism of an autotrophic sulfur oxidizing organism, Thiobacillus thiooxidans, Vogler, Umbreh and their collaborators (1, 2, 3, 4) have disclosed a new and striking ex ample of the general occurrence in nature of a linking between oxida tion and phosphate turnover. The processes of energy release through oxidation, in this case of sulfur, and of energy utilization could be separated. The oxidative phase was found to be accompanied by phos phate fixation, and the reductive phase of carbon dioxide fixation by a release of phosphate. O'Kane & Umbreit (5) also studied in some detail the more familiar coupling of glucose fermentation with phos phate fixation, using resting cells of Streptococcus fecalis. It may be emphasized that, parallel with the fermentative phosphate shift, an accumulation of potassium has been observed in several microorgan isms, e.g., in yeast (6) and in Escherichia coli.With radioactive phosphorus as indicator, Taurog, Chaikoff & Perlman (8) demonstrated a dependence of the synthesis of phospho lipids on oxidative metabolism in experiments with liver and kidney slices. Likewise, with brain slices (9), glucose increased the turnover four to five times. The permeation of phosphate into brain cells was followed and found largely dependent on carbohydrate metabolism. Analogous results with yeast are reported by Mullins (10).Efficiency of energy conversion.-The equivalent per mole of carbohydrate oxidized of energy-rich phosphate bonds, designated with ---'P h (11), had been determined by Belitzer & Tsibakova (12) and by Ochoa (13) to about 24 ---'P h, or 2 '--ph per atom of oxygen consumed. In such determinations fluoride is used to stabilize, more or less effectively, the generated phosphate esters; due to the unavoid able loss through dephosphorylation, these figures are to be considered minimum values. In a new series of experiments Ochoa (14) has determined now, rather reliably, the extent of the loss through dephos-1 Annu. Rev. Biochem. 1943.12:1-26. Downloaded from www.annualreviews.org Access provided by University of California -Davis on 02/03/15. For personal use only. Quick links to online content Further ANNUAL REVIEWS LIPMANNphorylation to be 30 to 40 per cent; he arrives at an average of 3 """' ph per atom of respired oxygen, or 36 �h per mole of carbohydrate.With 11 kca!. per bond (11) this makes an efficiency of 60 per cent for the conversion of available oxidation energy into utilizable phos-, phate bond energy. The question then arises how such a high yield of phosphate bonds can be explained in terms of a mechanism. Fol lowing the metabolic pathway of one half of the glucose passing from pyruvate tbrough the citric acid cycle, five energy-rich phosphate bonds may be derived as :follows : Two �h on the fermentation path way from glucose to pyruvate; two ,·-ph as acylphosphates, with the oxidation of the two a-keto acids, py:ruvic and ketoglutaric acids; and one �h, with Kalckar's enolphosphate...

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Studies in fat metabolism

Cited by 76 publications

References 2 publications

Fifty Years of Biochemistry. A Personal Account

Fifty Years of Biochemistry. A Personal Account

The Tricarboxylic acid cycle (revision number 25)

Biological Oxidations and Reductions

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