Studies of a Chloroplast-Localized Small Heat Shock Protein in Arabidopsis

Osteryoung, Katherine W.; Pipes, Brian L.; Wehmeyer, Nadja; Vierling, Elizabeth

doi:10.1007/978-3-642-79133-8_5

Cited by 4 publications

(5 citation statements)

References 27 publications

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“…Our results are in contrast to those of Osteryoung et al . (1994) which showed that an Arabidopsis chloroplast small heat shock protein accumulated in transgenic Arabidopsis at room temperature to 5–10 times the level normally seen after heat shock.…”

Section: Discussioncontrasting

confidence: 99%

“…Our results are in contrast to those of Osteryoung et al (1994) which showed that an Arabidopsis chloroplast small heat shock protein accumulated in transgenic Arabidopsis at room temperature to 5±10 times the level normally seen after heat shock. However, despite this extremely high level of Hsp accumulation, enhanced thermotolerance in these plants has never been reported.…”

Section: Enhancement Of Thermotolerance Does Not Require High Levels contrasting

confidence: 99%

“…1997 ). However, in Arabidopsis, over‐expression of a chloroplast small Hsp gene resulted in abundant expression of the protein, but no increase in thermotolerance was reported ( Osteryoung et al . 1994 ).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, expression of a rice small Hsp in Escherichia coli resulted in increased bacterial thermotolerance (Yeh et al, 1997). However, in Arabidopsis, over-expression of a chloroplast small Hsp gene resulted in abundant expression of the protein, but no increase in thermotolerance was reported (Osteryoung et al, 1994). Similarly, Schof¯et al (1987 reported differences in small Hsp mRNA levels, utilizing antisense and over-expression constructs in tobacco, but did not address the effects of these constructs on thermotolerance.…”

Section: Introductionmentioning

confidence: 99%

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Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡

Slovin²,

et al. 1999

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Summary We have determined that one small heat shock protein gene, encoding Hsp17.7, plays an important role in the ability of carrot cells and plants to survive thermal stress. Transgenic cells and regenerated plants were generated in which the carrot Hsp17.7 gene was either constitutively expressed (denoted CaS lines) or expressed as a heat inducible antisense RNA (denoted AH lines). Thermotolerance measurements demonstrated that CaS lines were more thermotolerant than vector controls and AH antisense lines were less thermo‐ tolerant than vector controls. RNA analysis demonstrated that Hsp17.7 mRNA was detectable, but not abundant, prior to heat shock in CaS cells, but not in vector control cells. Conversely, RNA analysis of antisense cells showed that, after heat shock, the amounts of mRNA for Hsp17.7 was moderately less abundant in AH cells than in vector controls. Analysis of protein synthesis in CaS cells did not indicate substantial synthesis or accumulation of Hsp17.7, or any small Hsp, at 23°C. However, in the most thermotolerant line, protein synthesis was maintained at a higher rate than in other cell lines at a more extreme heat shock (42°C). In contrast, antisense AH cells showed reduced synthesis of many Hsp, large and small. These results suggest that the Hsp17.7 gene plays a critical, although as yet not understood, role in thermotolerance in carrot. This represents the first demonstration of the ability to both increase and decrease thermotolerance by the manipulation of expression of a single gene.

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Section: Discussioncontrasting

confidence: 99%

Section: Enhancement Of Thermotolerance Does Not Require High Levels contrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡

Slovin²,

et al. 1999

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“…Transgenic tobacco expressed introduced HSP17.6 constitutively (Schoffl et al, 1987); however, due to the incompetence of the constitutive promoter, expression of the transgene was inhibited at high temperature and no conclusion was made concerning its effect on plant thermotolerance. No effect of overexpression of chloroplast-localized HSP21 on thermotolerance was observed in transgenic Arabidopsis plants (Osteryoung et al, 1994), possibly due to the overly high level of HSP21 accumulation.…”

mentioning

confidence: 97%

Genetic engineering for abiotic stress resistance in crop plants

Zhang

Klueva

Wang

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

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Drought, extreme temperatures and high salinity are major limiting factors for plant growth and crop productivity. In their quest to feed the ever-increasing world population, agricultural scientists have to contend with these adverse environmental factors. If crops can be redesigned to better cope with abiotic stress, agricultural production can be increased dramatically. Recent advances in understanding crop abiotic stress resistance mechanisms and the advent of molecular genetic technology allow us to address these issues much more efficiently than in the past. This paper reviews the most significant achievements of the genetic engineering approach to improving plant abiotic stress resistance and discusses future prospects in transgenic research. Improved resistance to drought, salinity and extreme temperatures has been observed in transgenic plants that express/overexpress genes regulating osmolytes, specific proteins, antioxidants, ion homeostasis, transcription factors and membrane composition. Although the results are not always consistent, these studies collectively foretell a scenario where biotechnology will arm our future crops with new tactics to survive in hostile environments. Further experiments are needed to determine if the achieved increases in stress tolerance are applicable to agriculture.

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The Chloroplast Small Heat Shock Protein in Transgenic Arabidopsis Thaliana

Härndahl¹,

Gustavsson²,

Buffoni³

et al. 1998

Photosynthesis: Mechanisms and Effects

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Studies of a Chloroplast-Localized Small Heat Shock Protein in Arabidopsis

Cited by 4 publications

References 27 publications

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡

Genetic engineering for abiotic stress resistance in crop plants

The Chloroplast Small Heat Shock Protein in Transgenic Arabidopsis Thaliana

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Studies of a Chloroplast-Localized Small Heat Shock Protein in Arabidopsis

Cited by 4 publications

References 27 publications

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance‡

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance‡

Genetic engineering for abiotic stress resistance in crop plants

The Chloroplast Small Heat Shock Protein in Transgenic Arabidopsis Thaliana

Contact Info

Product

Resources

About

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡

Modified expression of a carrot small heat shock protein gene, Hsp17.7, results in increased or decreased thermotolerance^‡