Studies of a Pyocin

Kageyama, Makoto; Ikeda, Kayoko; Egami, Fujio

doi:10.1093/oxfordjournals.jbchem.a127841

Cited by 47 publications

(30 citation statements)

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“…The chemical, physical, or biological characterization of the pyocin has been made and its resemblance to phages became more conspicuous (7)(8)(9). Similar structures were found by BERK et al (10) and by BRADLEY (11).…”

supporting

confidence: 59%

Isolation and Characterization of Pyocins From Several Strains of Pseudomonas Aeruginosa

Ito

Kageyama

Egami

1970

J. Gen. Appl. Microbiol.

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supporting

confidence: 59%

Isolation and Characterization of Pyocins From Several Strains of Pseudomonas Aeruginosa

Ito

Kageyama

Egami

1970

J. Gen. Appl. Microbiol.

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“…Quantitative tailocin activity assays were performed using a Poisson distribution killing method, adapted from Williams et al (14) and originally described by Kageyama et al (55). Briefly, tailocin serial dilutions in P-buffer were added to 10 9 CFU ml −1 target bacteria in TNB and incubated for 40 min at 37°C in triplicate.…”

Section: Methodsmentioning

confidence: 99%

A Broad-Host-Range Tailocin from Burkholderia cenocepacia

Yao

Duarte

et al. 2017

Appl Environ Microbiol

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The Burkholderia cepacia complex (Bcc) consists of 20 closely related Gram-negative bacterial species that are significant pathogens for persons with cystic fibrosis (CF). Some Bcc strains are highly transmissible and resistant to multiple antibiotics, making infection difficult to treat. A tailocin (phage tail-like bacteriocin), designated BceTMilo, with a broad host range against members of the Bcc, was identified in B. cenocepacia strain BC0425. Sixty-eight percent of Bcc representing 10 species and 90% of non-Bcc Burkholderia strains tested were sensitive to BceTMilo. BceTMilo also showed killing activity against Pseudomonas aeruginosa PAO1 and derivatives. Liquid chromatography-mass spectrometry analysis of the major BceTMilo proteins was used to identify a 23-kb tailocin locus in a draft BC0425 genome. The BceTMilo locus was syntenic and highly similar to a 24.6-kb region on chromosome 1 of B. cenocepacia J2315 (BCAL0081 to BCAL0107). A close relationship and synteny were observed between BceTMilo and Burkholderia phage KL3 and, by extension, with paradigm temperate myophage P2. Deletion mutants in the gene cluster encoding enzymes for biosynthesis of lipopolysaccharide (LPS) in the indicator strain B. cenocepacia K56-2 conferred resistance to BceTMilo. Analysis of the defined mutants in LPS biosynthetic genes indicated that an α-d-glucose residue in the core oligosaccharide is the receptor for BceTMilo.IMPORTANCE BceTMilo, presented in this study, is a broad-host-range tailocin active against Burkholderia spp. As such, BceTMilo and related or modified tailocins have potential as bactericidal therapeutic agents against plant- and human-pathogenic Burkholderia.

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“…Pyocin R was prepared from the Mitomycin-C lysate of P. aeruginosa P15 by the method of KAGEYAMA (9).…”

Section: Bacterial Strainsmentioning

confidence: 99%

Lipopolysaccharide of Pseudomonas Aeruginosa With Special Reference to Pyocin R Receptor Activity

Ikeda

Egami

1973

J. Gen. Appl. Microbiol.

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1) Lipopolysaccharide with pyocin R receptor activity was isolated from Pseudomonas aeruginosa P14 by the phenol method. Lipopolysaccharide was dissociated and fractionated into amino-sugar-rich fraction and lipopolysaccharide subunits by Sephadex G-100 gel filtration after heat treatment in the presence of sodium deoxycholate.2) The lipopolysaccharide subunits (mol. wt. 12,000-16,000) had no receptor activity in the presence of sodium deoxycholate, but they were reassociated in the absence of sodium deoxycholate and the activity was recovered. Therefore, the subunits may be regarded as the chemical entity of the receptor activity.3) Chemical analysis of the reassociated lipopolysaccharide and the carbohydrate fragment (mol. wt. 1,300-1,500) after acid treatment for the elimination of "lipid A" was carried out. The former contained glucose, rhamnose, heptose, glucosamine, galactosamine, quinovosamine, fucosamine, and a 2-keto-3-deoxy-sugar acid. The latter contained only glucose, rhamnose, heptose, galactosamine, 2-keto-3-deoxy-sugar acid, and phosphate. Lipopolysaccharideof Pseudomonas aeruginosa has been studied by HOMMA, one (F.E.) of the present authors, and their co-workers (1-4), and by GRAY, WILKINSON, and their co-workers (5-7) with different strains. The latter has studied mainly its chemical nature, and the former mainly biological activities such as toxicity, pyrogenicity, Schwartzman activity, and recently pyocin R receptor activity.Pyocin R is a bacteriocin produced by Pseudomonas aeruginosa P15 and 1 This constitutes Part II of a series entitled "Studies pyocin R." Part I: J.

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Studies of a Pyocin

Cited by 47 publications

References 0 publications

Isolation and Characterization of Pyocins From Several Strains of Pseudomonas Aeruginosa

Isolation and Characterization of Pyocins From Several Strains of Pseudomonas Aeruginosa

A Broad-Host-Range Tailocin from Burkholderia cenocepacia

Lipopolysaccharide of Pseudomonas Aeruginosa With Special Reference to Pyocin R Receptor Activity

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