Studies of a rice sterile mutant sstl from the TRIM collection

Abstract

Background Rice ( Oryza sativa ) is one of the main crops in the world, and more than 3.9 billion people will consume rice by 2025. Sterility significantly affects rice production and leads to yield defects. The undeveloped anthers or abnormal pollen represent serious defects in rice male sterility. Therefore, understanding the mechanism of male sterility is an important task. Here, we investigated a rice sterile mutant according to its developmental morphology and transcriptional profiles. Results An untagged T-DNA insertional mutant showed defective pollen and abnormal anthers as compared with its semi-sterile mutant ( sstl ) progeny segregates. Transcriptomic analysis of sterile sstl - s revealed several biosynthesis pathways, such as downregulated cell wall, lipids, secondary metabolism, and starch synthesis. This downregulation is consistent with the morphological characterization of sstl - s anthers with irregular exine, absence of intine, no starch accumulation in pollen grains and no accumulated flavonoids in anthers. Moreover, defective microsporangia development led to abnormal anther locule and aborted microspores. The downregulated lipids, starch, and cell wall synthesis-related genes resulted in loss of fertility. Conclusions We illustrate the importance of microsporangia in the development of anthers and functional microspores. Abnormal development of pollen grains, pollen wall, anther locule, etc. result in severe yield reduction. Electronic supplementary material The online version of this article (10.1186/s40529-019-0260-3) contains supplementary material, which is available to authorized users.

Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics

“…This phenomenon is due to spontaneous outcrossing occurring in different generations in the rice fields [54]. While screening for novel phenotypes in Taiwan Rice Insertional Mutagenesis (TRIM) population, Chang et al 2019, identified a semi-sterile mutant ( sstl ), however, it was later found to be associated with an un-tagged T-DNA line [55].…”

Genetic analysis technology based on retrotransposons via high-throughput sequencing platform

“…出す．よって，劇的にコピー数を増加させる可能性があ り，植物ゲノムの拡大にも寄与してきたことが推察され る Bennetzen 1999, Bento et al 2013 (Kumar and Bennetzen 1999, Feschotte et al 2002, Havecker et al 2004 (Kumar and Hirochika 2001, Schulman et al 2004, Kalendar et al 2011, Poczai et al 2013 れる (Flavell et al 1998, Kalendar et al 1999, Waugh et al 1997, Syed et al 2005 (Kumar and Bennetzen 1999, Feschotte et al 2002, Slotkin and Martienssen 2007, Lisch 2009) ．その理由としては，変異を引き起こす可能 性のあるトランスポゾンに対して，宿主ゲノムが DNA のメチル化やクロマチン修飾等のエピジェネティック な制御機構を進化させてきたためであると考えられてい る ( Slotkin andMartienssen 2007, Lisch 2009 (Marquet et al 1995, Mak and Kleiman 1997, Kelly et al 2003, Hizi 2008 (Ukoskit and Thompson 1997, Kriegner et al 2003, Cervantes-Flores et al 2008, Zhao et al 2013 (Ukoskit and Thompson 1997, Kriegner et al 2003, Cervantes-Flores et al 2008, Chang et al 2009, Li et al 2010, Zhao et al 2013…”