Studies of Adipose Tissue in Man

Hirsch, Jules; Farquhar, John W.; Ahrens, E. H.; Peterson, Malcolm L.; Stoffel, Wilhelm

doi:10.1093/ajcn/8.4.499

Cited by 492 publications

(69 citation statements)

References 18 publications

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“…All differences between low fat and high fat diets are statistically insignificant (P Ͼ 0.01) except percent de novo VLDL TG. acids (predominantly 16:0) is also present in the adipose tissue of newborn infants who synthesize most of their fat in utero from maternal carbohydrate (20,29).…”

Section: Discussionmentioning

confidence: 99%

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Human fatty acid synthesis is stimulated by a eucaloric low fat, high carbohydrate diet.

Hudgins¹,

Hellerstein²,

Seidman³

et al. 1996

J. Clin. Invest.

328

251

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Section: Discussionmentioning

confidence: 99%

“…Subcutaneous adipose tissue from the abdominal and gluteal regions was aspirated through a needle attached to a syringe after local anesthesia with 1% lidocaine within 3 wk of admission and at the end of each diet (20).…”

Section: Methodsmentioning

confidence: 99%

Human fatty acid synthesis is stimulated by a eucaloric low fat, high carbohydrate diet.

Hudgins¹,

Hellerstein²,

Seidman³

et al. 1996

J. Clin. Invest.

328

251

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“…Work on human adipose tissue, on the other hand, has been very limited and has chiefly dealt with its composition as affected by various diets (4)(5)(6). A study of the metabolic activity of human adipose tissues has been reported by Gellhorn and Marks (7).…”

mentioning

confidence: 99%

“…The tissue most easily obtained by biopsy from humans for metabolic studies is the subcutaneous adipose tissue (5,6). It has to be established whether the analysis of the metabolic activity of this type of tissue yields results representative of the over-all activity of adipose tissues.…”

mentioning

confidence: 99%

Fatty-Acid Metabolism by Human Adipose Tissues*

Hamosh¹,

Hamosh²,

Bar-Maor³

et al. 1963

J. Clin. Invest.

118

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The metabolic activity of rat and mouse adipose tissues has been much investigated and the work extensively reviewed (1-3). Work on human adipose tissue, on the other hand, has been very limited and has chiefly dealt with its composition as affected by various diets (4-6). A study of the metabolic activity of human adipose tissues has been reported by Gellhorn and Marks (7). They compared the incorporation of acetate-1-C14 into lipids of human subcutaneous adipose tissue with that of lipomas and found a much higher activity in the latter tissue.The tissue most easily obtained by biopsy from humans for metabolic studies is the subcutaneous adipose tissue (5, 6). It has to be established whether the analysis of the metabolic activity of this type of tissue yields results representative of the over-all activity of adipose tissues. We therefore undertook to compare the metabolic activity and composition of two types of human adipose tissues, subcutaneous and omental, obtained during surgical intervention. METHODSAdipose tissues were obtained from surgical patients with normal liver tests and normal blood glucose, cholesterol, and FFA. Twenty-two patients (nine men and thirteen women) ranging in age from 25 to 62 years were studied. After a fast of 8 to 12 hours, surgery was performed under general anesthesia consisting of pentobarbital, nitrous oxide, and cyclopropane. No ether was used. The adipose tissues obtained were kept in calcium-free Krebs-Ringer-phosphate buffer (8) at room temperature. Preliminary experiments with rat adipose tissues have shown that tissues kept on ice before the incubation showed decreased palmitate uptake and esterification. Preservation at room temperature for 3 hours did not cause any measurable decrease in activity *

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“…Hirsch, Farquhar, Ahrens, Peterson and Stoffel (9) suggested that since 18: 2 is an essential fatty acid, the amount present in adipose tissue reflects the amount consumed over a period of years. Dayton, Hashimoto, and Pearce (10) have shown that after 5 yr on a corn oil diet, adipose tissue 18: 2 correlates with adherence to diet.…”

Section: Introductionmentioning

confidence: 99%