Studies of antilymphocyte antisera produced against normal and rheumatoid arthritis lymphocytes

Abstract

Rabbit antisera were made against pooled normal human lymphocytes and against those from patients with active rheumatoid arthritis. Sera were subsequently absorbed to remove antibody to human erythrocytes and plasma proteins. Most antilymphocyte antisera showed considerable cytotoxic or complement fixing antibody after immunization, however a striking deficit (6 of 12 animals) in the activity of the antisera against RA lymphocytes was noted when blast transformation of lymphocytes was used as a test of antilymphocyte serum (ALS) antibody.

Studies of T- and B-Lymphocytes in Patients with Connective Tissue Diseases

“…Peripheral blood lymphocytes were collected from blood drawn in heparinized syringes and cells separated using a FicollHypaque gradient as previously described (31,32 Fig. 3.…”