Studies of Enzyme Changes During Sporulation in <i>Bacillus cereus</i>, Using Starch Gel Electrophoresis

Baillie, A. H.; Norris, J. R.

doi:10.1111/j.1365-2672.1963.tb01163.x

Cited by 43 publications

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“…It is common to find that isoenzyme patterns change as organisms age and undergo morphological changes. For example, esterase and catalase patterns of Bacillus cereus undergo changes during sporulation (Baillie and Norris 1963). The acid phosphatase and esterase patterns of Dictyostelium discoideum alter as the vegetative myxamoebae differentiate into spores (Solomon, Johnson, and Gregg 1964).…”

Section: Discussionmentioning

confidence: 99%

Proteins and Catalase Isoenzymes from Fusarium Solani and their Taxonomic Significance

Hall¹

1967

Aust. Jnl. Of Bio. Sci.

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SummaryProteins 'and catalase isoenzymes from saprophytic and parasitic forms of FUBarium Bolani were separated electrophoretically in starch gels. Protein patterns of an isolate of F. Bolani f. pisi showed little response to altered cultural conditions but catalase patterns altered with culture age, illumination of culture, number of conidia in the inoculum, and portion of growth harvested. Under optimum conditions a maximum of four catalase bands was detected. Evidence is presented that hyphae and spores of this isolate have different catalase patterns and that these patterns are influenced by light intensity.Under conditions optimum for the above isolate of F. Bolani f. pisi, culturally and pathogenically distinct isolates of F. Bolani produced essentially identical protein patterns but markedly different catalase patterns. The taxonomic implications of this are discussed.

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Section: Discussionmentioning

confidence: 99%

Proteins and Catalase Isoenzymes from Fusarium Solani and their Taxonomic Significance

Hall¹

1967

Aust. Jnl. Of Bio. Sci.

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“…No organisms with this esterase pattern have so far been isolated that were not of serotype 4B. Two isolates (see below) had slightly modified esterase patterns with considerably less intense or-esterase bands (Baillie & Norris, 1963).…”

Section: Characterization Of the Isolatesmentioning

confidence: 97%

“…Esterase analysis was carried out on ultrasonic disintegrates of vegetative cells by the starch gel electrophoretic method of Norris & Burges (1963) using the modified Poulik gel buffer of Baillie & Norris (1963).…”

Section: Identi$cation Of Isolatesmentioning

confidence: 99%

The Ecology of Serotype 4B of Bacillus thuringiensis

Norris

1969

Journal of Applied Bacteriology

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Summary Twentyfive separate isolates of serotype 4B of the aerobic sporeforming insect pathogen, Bacillus thuringiensis, have been obtained over the period 1961–68. Most of the isolates of this particular serotype were obtained from storage products insects infesting such places as grain stores, flour mills or ships carrying grain materials. In one instance the organism was isolated from insects infesting Guinea corn growing in the field. In comparison with the other serotypes of this bacillus the association with stored products environments is striking and, on several occasions, it has been possible to trace the distribution of the bacteria by movements of infested materials from one part of the world to another.

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“…A study of the esterase profiles from various stages of the growth cycle of BacilZus cereus has revealed a changing pattern of enzymes (Baillie & Norris, 1963). A single esterase band was demonstrated in young cultures and the development of a second band was noted after I I h. growth.…”

Section: Introductionmentioning

confidence: 99%

A Study of the Esterases and Their Function in Candida lipolytica, Aspergillus niger and a Yeast-like Fungus

Lloyd

Morris

Smith

1970

Journal of General Microbiology

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S U M M A R YEsterases, determined by polyacrylamide gel electrophoresis, were present when Candida lipolytica was grown in a liquid, shaken, glucose-mineral salts medium. Intracellular esterase activity increased during growth, but extracellular esterase activity was small and increased only marginally. Esterases were not detected in organisms grown on solid glucose-mineral salts medium, but were present when glycerol tributyrin replaced glucose.At the onset of asexual sporulation in a yeast-like fungus, three new esterases occurred. Intracellular esterase activity increased, intracellular lipid utilization occurred, and the respiratory quotient decreased. No extracellular esterase activity was detected.Esterases were only detected in Aspergillus niger at late stages of conidiation when intracellular lipid decreased. Esterase activity was not detected in the mitochondria or in the cell-free growth medium.Esterases of all organisms tested hydrolysed glycerol tributyrin and were arbitrarily classified as lipases ; intracellular lipid decreased with increase in esterase activity function. Esterase and profile changes may reflect a role of lipids in sporulation and physiological ageing.

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Studies of Enzyme Changes During Sporulation in Bacillus cereus, Using Starch Gel Electrophoresis

Abstract: Summary Starch gel electrophoresis showed that Bacillus cereus esterase and catalase are not single enzymes but multimolecular species and that they undergo changes during sporulation. Heat resistant spore catalase was detected in vegetative cells before mature spores were present.

Cited by 43 publications

References 5 publications

Proteins and Catalase Isoenzymes from Fusarium Solani and their Taxonomic Significance

Proteins and Catalase Isoenzymes from Fusarium Solani and their Taxonomic Significance

The Ecology of Serotype 4B of Bacillus thuringiensis

A Study of the Esterases and Their Function in Candida lipolytica, Aspergillus niger and a Yeast-like Fungus

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