In this study, the impact of assimilating MWHS2 radiance data under different background conditions on the analyses and deterministic prediction of the Super Typhoon Muifa case, which hit China in 2022, was explored. The fifth-generation European Centre for Medium-Range Weather Forecasts (ECMWF) Reanalysis v5 (ERA5) data and the Global Forecast System (GFS) analysis data from the National Centers for Environmental Prediction (NCEP) were used as the background fields. To assimilate the Microwave Humidity Sounder II (MWHS2) radiance data into the numerical simulation experiments, the Weather Research and Forecasting (WRF) model and its three-dimensional variational data assimilation system were employed. The results show that after the data assimilation, the standard deviation and root-mean-square error of the analysis significantly decrease relative to the observation, indicating the effectiveness of the assimilation process with both background fields. In the MWHS_GFS experiment, a subtropical high-pressure deviation to the east is observed around the typhoon, resulting in its northeast movement. In the differential field of the MWHS_ERA experiment, negative sea-level pressure differences around the typhoon are observed, which increases its intensity. In the deterministic predictions, assimilating the FY3D MWHS2 radiance data reduces the typhoon track error in the MWHS_GFS experiment and the typhoon intensity error in the MWHS_ERA experiment. In addition, it is found that the Community Radiative Transfer Model (CRTM) and the Radiative Transfer for Tovs (RTTOV) model show similar performance in assimilating MWHS2 radiance data for this typhoon case. It seems that the data assimilation experiment with the CRTM significantly reduces the typhoon track error than the experiment with the RTTOV model does, while the intensity error of both experiments is rather comparable.