Studies of GH Secretion in Mice by a Homologous Radioimmunoassay for Mouse GH1

Sinha, Y. N.; Selby, F. W.; Lewis, U. J.; Vanderlaan, W. P.

doi:10.1210/endo-91-3-784

Cited by 158 publications

(56 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gene expression and fat storage in growth-selected mice D Timtchenko et al et al, 21 in the laboratory of F. Talamantes (University of California, Santa Cruz, CA). The IGF-1 quanti®ca-tion was based on a RIA, in which the interference of IGF binding proteins is blocked by incubation in the presence of excess IGF-2.…”

Section: Measurements Of Serum Proteinsmentioning

confidence: 99%

Fat storage capacity in growth-selected and control mouse lines is associated with line-specific gene expression and plasma hormone levels

Timtchenko

Kratzsch

Sauerwein³

et al. 1999

Int J Obes

View full text Add to dashboard Cite

OBJECTIVE: For a detailed understanding of the complex traits growth and fat storage, a dissection into single genetic entities is mandatory. Therefore, blood plasma concentrations of hormones and the expression of selected genes were measured in extremely differentiated mouse lines. Genes were selected as candidates which might in¯uence the complex traits body weight and fat accumulation, and which are located in chromosomal regions recently identi®ed to affect trait differences between the lines. SUBJECTS AND MEASUREMENTS: The mouse lines were selected for high body weight (DU6), high carcass protein content (DU6P) and unselected controls (DUKs). In the selected lines DU6 and DU6P, mean body weights at the age of six weeks were about twice as high as the DUKs, whereas total fat weight was increased 2.2-fold in DU6 mice compared to DU6P and 3.2-fold in comparison to DUKs. Blood plasma concentrations of insulin-like growth factor 1 (IGF-1), growth hormone (GH), insulin and leptin, were measured in all lines at three weeks and at six weeks of age. Expression patterns of the genes encoding growth hormone (Gh), insulin-like growth factor 1 (Igf1), lipoprotein lipase (Lpl), glycerolphosphate dehydrogenase 1 (GDC-1), and adipocyte protein 2 (Ap2) were analyzed by Northern blot hybridization. RESULTS: In line DU6, highly signi®cant increased concentrations of insulin and leptin were observed at six weeks of age; at this stage, IGF-1 concentrations were elevated in the two selected lines compared to controls with maximal concentrations of IGF-1 and GH in DU6P. The amount of mRNA for GH in the pituitary gland, for Igf1 in the liver and for LPL in epididymal fat tissue was signi®cantly elevated in the two selected lines compared to controls at the age of three weeks, but not at six weeks. IGF-1 and GDC-1 mRNA concentrations were signi®cantly higher in the DU6 mice than in the DU6P (P`0.01) and the DUKs (P`0.001) mice examined at both ages. CONCLUSIONS: The results prove line-speci®c concentrations of the analyzed hormones and the transcription amounts of Gh, Igf1, GDC-1 and Lpl. The measured differences are either direct genetic effects or secondary changes, resulting from different food consumption.

show abstract

Section: Measurements Of Serum Proteinsmentioning

confidence: 99%

Fat storage capacity in growth-selected and control mouse lines is associated with line-specific gene expression and plasma hormone levels

Timtchenko

Kratzsch

Sauerwein³

et al. 1999

Int J Obes

View full text Add to dashboard Cite

show abstract

“…DEAE-dextran, benzamidine, aprotinin, leupeptin, pepstatin, molecular weight standards for SDS-PAGE, and trypsin were purchased from Sigma (Deisenhofen, Germany). Polyclonal antimouse GH antibody, which cross-reacts with rat GH, was a generous gift of Dr. Sinha (22).…”

Section: Methodsmentioning

confidence: 99%

“…Transfections were performed using a calcium phosphate-DNA precipitation procedure (27). To establish stably expressing cell lines, cells were selected with 0.4 mg/ml G418 (Life Technologies, Inc.) for 2 weeks after which individual clones were isolated and screened for expression of the chimeric protein with a polyclonal monkey anti-mouse GH antibody which cross-reacts with rat GH (22). For analysis of cell surface polarity, cells were grown on Transwell filters (24 mm, 0.4 m) (Becton Dickinson GmbH, Heidelberg, Germany) for 1 week after confluence.…”

Section: Expression Of Chimeras Of the Rat Growth Hormone Fused To Vamentioning

confidence: 99%

See 1 more Smart Citation

Characteristics and Structural Requirements of Apical Sorting of the Rat Growth Hormone through the O-Glycosylated Stalk Region of Intestinal Sucrase-isomaltase

Spodsberg¹,

Alfalah²,

Naim³

2001

Journal of Biological Chemistry

View full text Add to dashboard Cite

The apical sorting of the small intestinal membrane glycoprotein sucrase-isomaltase (SI) depends on the presence of O-linked glycans and the transmembrane domain. Here, we investigate the role of O-glycans carried by the Ser/Thr-rich stalk region of SI as an apical sorting signal and evaluate the spatial requirements for an efficient recognition of this signal. Several hybrid proteins are generated comprising the unsorted and unglycosylated protein, the rat growth hormone (rGH), fused to either the transmembrane domain of SI (GH-SI TM ), or the transmembrane and the stalk domains (GH-SI SR/TM ). Both constructs are randomly distributed over the apical and basolateral membranes of MDCK cells indicating that neither the transmembrane domain nor the O-glycans are sufficient per se for an apical delivery. Only when a polyglycine spacer is inserted between the stalk region of SI and the luminal part of rGH in the GH-SI Gly/SR/TM fusion protein does efficient apical sorting of an O-glycosylated protein as well as a time-dependent association with detergent-insoluble lipid microdomains occur. Obviously, the polyglycine spacer facilitates the accessibility of the O-glycans in GH-SI Gly/SR/TM to a putative sorting receptor, whereas these glycans are inadequately recognized in GH-SI SR/ TM. We conclude that the O-glycans in the stalk region of SI act as an apical sorting signal within a sorting machinery that comprises at least a carbohydrate-binding protein and fulfills specific spatial requirements provided, for example by a polyglycine spacer in the context of rGH or the P-domain within the SI enzyme complex.

show abstract

“…Each plasma sample was assayed in triplicate for prolactin and growth hormone using NIAMDD reagents. The procedure [10] was employed for prolactin determinations and the procedure [11] for growth hormone. only at an enkephalin analog dose of 1 mg/kg (fig.l); morphine sulfate did not release prolactin at either of the levels studied.…”

Section: Biological Testingmentioning

confidence: 99%

Stimulation of growth hormone and prolactin release by a potent enkephalin analog

Lien¹,

Clark²,

McGregor³

1978

FEBS Letters

View full text Add to dashboard Cite

Studies of GH Secretion in Mice by a Homologous Radioimmunoassay for Mouse GH1

Cited by 158 publications

References 0 publications

Fat storage capacity in growth-selected and control mouse lines is associated with line-specific gene expression and plasma hormone levels

Fat storage capacity in growth-selected and control mouse lines is associated with line-specific gene expression and plasma hormone levels

Characteristics and Structural Requirements of Apical Sorting of the Rat Growth Hormone through the O-Glycosylated Stalk Region of Intestinal Sucrase-isomaltase

Stimulation of growth hormone and prolactin release by a potent enkephalin analog

Contact Info

Product

Resources

About