Studies of growth &amp; metabolism of a barley mutant lacking chlorophyll b

Highkin, H. R.; Frenkel, Albert W.

doi:10.1104/pp.37.6.814

Cited by 73 publications

(22 citation statements)

References 10 publications

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“…Previous studies with a mutant of barley (Hordeum vulgare L.) devoid of chlorophyll b indicated that chlorophyll b is not essential for photosynthetic activity in a higher plant (6,11,13). Photosynihetic rates obtained with the mutant plants did not differ significantly from those obtained in normal plants (13).…”

mentioning

confidence: 78%

“…Photosynihetic rates obtained with the mutant plants did not differ significantly from those obtained in normal plants (13). Hill reaction measurements on isolated chloroplasts showed that the mutant chloroplasts were more active per mg of total chlorophyll if assayed at saturating light intensities, but less active at low light intensities (6).…”

mentioning

confidence: 81%

“…It is interesting to compare our chlorophylldeficient pea mutant with the barley mutant lacking chlorophyll b, described previously (6,11,13), and the chlorophyll-deficient tobacco mutant (SU/Su) studied by Schmid and Gaffron (18,19) and Homann and Schmid (14). The mutant barley leaves have about 70 % of the total chlorophyll of the leaves of normal barley plants (6) while the mutant tobacco has about 20 to 30 % of the chlorophyll of the normal tobacco (14).…”

Section: Discussionmentioning

confidence: 99%

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Photosynthetic Studies on a Pea-mutant Deficient in Chlorophyll

1969

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Abstract. A chlorophyll-deficient mutant of pea (Pisum sativum) was found as a spontaneous mutation of the variety Greenfeast. Total chlorophyll of the mutant leaves was about one-half that of normal pea leaves per mg dry weight, and the ratio of chl a:chl b ranged from 10 to 18, compared with 3 for normal pea. In each generation the mutant plants gave rise to normal and mutant plants and lethal plants with yellow leaves.For a normal pea plant, CO2 uptake was saturated at about 60,000 lux, whereas with mutant leaves, the rate of CO2 uptake was still increasing at 113,000 lux. At 113,000 lux the mutant and normal leaves showed similar rates of CO2 fixation per unit area of leaf surface, but on a chlorophyll basis the mutant leaves were twice as active. Hill reaction measurements on isolated chloroplasts also showed that the mutant chloroplasts werc saturated at higher intensities than the normal, and that the activity of the mutant was at least double that of the normal on a chlorophyll basis.It is suggested that the photosynthetic units of the mutant chloroplasts contain about half the number of chlorophyll molecules as compared to the normal photosynthetic units.Electron microscopy of leaf sections of normal and mutant leaves showed that the mutant chloropla,sts oontain fewer lamellae per chloroplast and fewer lamellae per granum. The lethal chloroplasts, which were virtually devoid of chlorophyll, were characterized by an absence of grana.

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confidence: 78%

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confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Photosynthetic Studies on a Pea-mutant Deficient in Chlorophyll

1969

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“…Estimation of carbohydrates: Reducing and non-reducing sugars were extracted from shoot and root from all the test treatments were estimated by Highkin and Frankel method [26]. The carbohydrate contents were estimated on 15 th , 30 th , 45 th and 60 th days of plant growth.…”

Section: Physiological Parametersmentioning

confidence: 99%

Integrated Effect of Rhizobium and Azotobacter Cultures on the Leguminous Crop Black Gram (Vigna mungo)

Tiwari¹,

Chauhan²,

Singh³

et al. 2017

Adv Crop Sci Tech

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A pot experiment was performed to evaluate the integrated effect of Rhizobium and Azotobacter sp. on the plant growth, nodule appearance, no of leaf, shoot length, root length, chlorophyll contents and carbohydrate content in .2) and chlorophyll content (1.3 mg/g) and reducing (867.4 µg/g) and non-reducing sugar (1905.5 µg/g) content per plant biomass respectively. The Azotobacter and Rhizobium sp. have friendly associations and they have different physiology and habitat. Therefore, they help plant growth promotion by them own system. Therefore, such combination can be recommended for field application for sustainable agriculture. Excessive application of chemical fertilizers causes environmental and economic problems; hence the use of PGPR and Rhizobium bacteria can be acceptable due to cut contribution expenditure, increase in grain yield and environmental friendly.

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“…Smith (29) worked with etiolated barley leaves studying the development of chlorophyll and oxygen-evolving power, and observed that chlorophyll b was not necessary for oxygen evolution. Highkin and Frenkel (13) Analyses. Qualitative differences in pigment content between the high and low light cultures were observed before extraction by using the opal glass technique (27).…”

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confidence: 99%