Studies of interaction of emodin and DNA in the presence of ethidium bromide by spectroscopic method

Bi, Shuyun; Zhang, Hanqi; Qiao, Chunyu; Sun, Ying; Liu, Chunming

doi:10.1016/j.saa.2007.03.017

Cited by 146 publications

(64 citation statements)

References 33 publications

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“…Fluorescence probe should be utilized because of the non-fluorescence of Tat (49-57) and the minimal practical usefulness of intrinsic fluorescence from DNA [24] . In this work, EB was used as the fluorescence probe.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis, DNA-Binding and Antibacterial Activity of the Cell-Penetrating Peptide HIV-1 Tat (49-57)

Lv¹,

Duan²,

Lu³

et al. 2017

pharmaceutical-sciences

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Section: Resultsmentioning

confidence: 99%

“…The intercalative binding mode can stabilize the helix structure of DNA and causes the melting temperature to increase by about 5-8°, but the electrostatic and groove binding interactions cause no obvious or minimal change in T m [15,24] . The melting curves ( fig.…”

Section: Resultsmentioning

confidence: 99%

Synthesis, DNA-Binding and Antibacterial Activity of the Cell-Penetrating Peptide HIV-1 Tat (49-57)

Lv¹,

Duan²,

Lu³

et al. 2017

pharmaceutical-sciences

View full text Add to dashboard Cite

“…IC 50 values were estimated for chronic myelogenous leukemia cell line, K562. Figure 1 21,40 that is, both complexes (especially Pd(II) complex) can denature DNA at very low concentrations. Thus, if these complexes are used as anti-tumor agents, low doses will be needed, this may have fewer side effects.…”

Section: Resultsmentioning

confidence: 97%

“…Sodium salt was used to adjust the ionic strength of the solution. 21 A solution of CT-DNA gave a ratio of UV absorbance at 260 and 280 nm more than 1.8, indicating that CT-DNA was sufficiently free from protein.…”

Section: Methodsmentioning

confidence: 98%

DNA-Binding and Thermodynamic Parameters, Structure and Cytotoxicity of Newly Designed Platinum(II) and Palladium(II) Anti-Tumor Complexes

Mansouri‐Torshizi

Saeidifar²,

Khosravi³

et al. 2011

Bulletin of the Korean Chemical Society

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The complexes [Pd(bpy)(Hex-dtc)]NO 3 and [Pt(bpy)(Hex-dtc)]NO 3 (bpy is 2,2'-bipyridine and Hex-dtc is hexyldithiocarbamato ligands) were synthesized and characterized by elemental analysis and spectroscopic studies. The cytotoxicity assay of the complexes has been performed on chronic myelogenous leukemia cell line, K562, at micromolar concentration. Both complexes showed cytotoxic activity far better than that of cisplatin under the same experimental conditions. The binding parameters of the complexes with calf thymus DNA (CT-DNA) was investigated using UV-visible and fluorescence techniques. They show the ability of cooperatively intercalating in CT-DNA. Gel filtration studies demonstrated that platinum complex could cleave the DNA. In the interaction studies between the Pd(II) and Pt(II) complexes with CT-DNA, several binding and thermodynamic parameters have been determined, which may provide deeper insights into the mechanism of action of these types of complexes with nucleic acids.

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“…In previous studies, UV/Visible absorption and fluorescence spectroscopy have been applied as analytical tools to study the interaction of Emodin with solvents (Pal and Jana 1993;Nguyen et al 2008;Sevilla et al 2009;da Cunha et al 2014), with biological environments like human serum albumin (HSA) (Fabriciova et al 2004;Vargas et al 2004), DNA (Wang et al 2006;Saito et al 2012;Bi et al 2008), phospholipid bilayer (Alves et al 2004) and with other environments Fig. 1 Chemical structure and atomic numbering of Emodin in its neutral form (EMH) like porous silicon (Hernandez et al 2012), silver nanoparticles (Sevilla et al 2009(Sevilla et al , 2010 and some metal ions (Pal and Jana 1993).…”

Section: Introductionmentioning

confidence: 99%

Experimental and theoretical studies of emodin interacting with a lipid bilayer of DMPC

et al. 2017

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Emodin is one of the most abundant anthraquinone derivatives found in nature. It is the active principle of some traditional herbal medicines with known biological activities. In this work, we combined experimental and theoretical studies to reveal information about location, orientation, interaction and perturbing effects of Emodin on lipid bilayers, where we have taken into account the neutral form of the Emodin (EMH) and its anionic/deprotonated form (EM − ). Using both UV/Visible spectrophotometric techniques and molecular dynamics (MD) simulations, we showed that both EMH and EM − are located in a lipid membrane. Additionally, using MD simulations, we revealed that both forms of Emodin are very close to glycerol groups of the lipid molecules, with the EMH inserted more deeply into the bilayer and more disoriented relative to the normal of the membrane when compared with the EM − , which is more exposed to interfacial water. Analysis of several structural properties of acyl chains of the lipids in a hydrated pure DMPC bilayer and in the presence of Emodin revealed that both EMH and EM − affect the lipid bilayer, resulting in a remarkable disorder of the bilayer in the vicinity of the Emodin. However, the disorder caused by EMH is weaker than that caused by EM − . Our results suggest that these disorders caused by Emodin might lead to distinct effects on lipid bilayers including its disruption which are reported in the literature.

show abstract

Studies of interaction of emodin and DNA in the presence of ethidium bromide by spectroscopic method

Cited by 146 publications

References 33 publications

Synthesis, DNA-Binding and Antibacterial Activity of the Cell-Penetrating Peptide HIV-1 Tat (49-57)

Synthesis, DNA-Binding and Antibacterial Activity of the Cell-Penetrating Peptide HIV-1 Tat (49-57)

DNA-Binding and Thermodynamic Parameters, Structure and Cytotoxicity of Newly Designed Platinum(II) and Palladium(II) Anti-Tumor Complexes

Experimental and theoretical studies of emodin interacting with a lipid bilayer of DMPC

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