Deep eutectic solvents (DESs) are considered to play an important role in green chemistry and other technological fields as an alternative to organic solvents. The present study reports measurements of density (ρ), speed of sound (u), dynamic viscosity (η), and electrical conductivity (κ) and investigates physicochemical properties of choline chloride/acetic acid (ChCl/AcA DES) and its binary mixtures with dimethyl sulfoxide (DMSO) over the entire composition and temperature (298.15−353.15 K) range. The density data are well fitted by a second-degree polynomial equation in T. DES/DMSO mixtures exhibit negative excess molar volume and isentropic compressibility deviation with a minimum in respective curves at x 1 ≈ 0.15 (x 1 is the mole fraction of DES in the mixture), which became deeper with increasing temperature. The ChCl/AcA DES and DMSO curves for excess partial molar volume cross each other at x 1 ≈ 0.15, showing that the packing effect is dominant over specific interactions. A similar behavior is observed for excess molar viscosity, showing the minima at x 1 ≈ 0.62, and substantiates volumetric results. The temperature dependence of viscosity and conductivity is well described by the Vogel−Fulcher−Tammann (VFT) equation.