Studies of lectin binding to normal and neoplastic lymph nodes. II. Non-Hodgkin's lymphoma

Bramwell, Vivien; Crowther, Derek; Gallagher, John T.; Stoddart, R. W.

doi:10.1038/bjc.1982.242

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…In general the lectin staining patterns of Reed-Sternberg cells most closely resembled macrophages, rather than normal or even proliferating non-malignant lymphocytes. However, the malignant lymphocytes present in some types of non-Hodgkin lymphoma (Bramwell et al, 1982) closely resembled the Reed-Sternberg cells, and derivation from a transformed lymphocyte remains possible.…”

Section: Galnac Neunacmentioning

confidence: 99%

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Studies of lectin binding to normal and neoplastic lymphoid tissues. I. Normal nodes and Hodgkin's disease

Bramwell¹,

Crowther²,

Gallagher³

et al. 1982

Br J Cancer

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Lectins are proteins which have the ability to interact specifically with carbohydrate residues of glycoproteins and other glycoconjugates. The staining patterns of 10 fluorescein conjugated lectins (F-Con A, F-LCA, F-RCA, F-WGA, F-PHA, F-PWM, F-LTA, F-SBA, F-PNA, F-DB) and a protease inhibitor (F-LA) have been studied in histological sections of 11 normal or reactive lymph nodes and 6 nodes and one skin biopsy involved by Hodgkin's disease. On the basis of the patterns of lectin binding, and current knowledge of their saccharide specificities, we found that within germinal centres there is an orderly carbohydrate rich extracellular matrix which contains a higher concentration of GlcNAc and terminal Gal residues than the surface membranes of component cells. This suggests active secretion rather than simple membrane shedding, and it is possible that this pericellular domain plays a part in the regulation of the proliferative response, or controls migration of lymphocytes in and out of the germinal centre. Lectin binding in Reed-Sternberg cells suggests that the huge nucleoli contain glycoconjugates of diverse structure, which may be linked with their failure to undergo cytokinesis. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5

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Section: Galnac Neunacmentioning

confidence: 99%

“…A particular advantage of a histochemical technique is the opportunity to examine cells in their natural environment. This paper compares nodes not involved by lymphoma and those involved by Hodgkin's disease, and the following paper (Bramwell et al, 1982) describes the findings in non-Hodgkin's lymphoma.…”

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Studies of lectin binding to normal and neoplastic lymphoid tissues. I. Normal nodes and Hodgkin's disease

Bramwell¹,

Crowther²,

Gallagher³

et al. 1982

Br J Cancer

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Comprehensive lectin histochemistry of normal and neoplastic human choroid plexus cells: alternation of lectin-binding patterns through neoplastic transformation

et al. 1991

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Lectin histochemistry of the normal and neoplastic human choroid plexus cells [six choroid plexus papillomas (CPPs) and three choroid plexus carcinomas (CPCs)] was performed using eight representative lectins to study the development of sugar chain structures and also to determine whether lectins were useful for a histopathological diagnosis of choroid plexus neoplasms (CPNs). The normal choroid plexus cells reacted with Ricinus communis (RCA-I). Canavalia ensiformis (Con A), Limax flavus (LFA) and Triticum vulgaris (WGA), while Arachis hypoaea (PNA) stained them only after the removal of sialic acid. Human fetal choroid plexus cells at 8 weeks gestation already showed the same lectin-binding patterns as adult ones. All CPNs were stained by RCA-I and Con A in a similar manner as the normal choroid plexus cells. Although seven CPNs were positive for LFA, two CPCs were not stained by LFA, which bound to sialic acid. Two LFA-positive CPPs were stained by PNA before the removal of sialic acid. Moreover, unlike the normal choroid plexus cells, Ulex europaeus-, Glycine maximus- and Dolichos biflorus-binding sites often appeared, and WGA-binding sites of three CPNs remained even after sialic acid removal. In conclusion, the glycosialylation in normal choroid plexus cells was completed during the early embryonic stage. The lectin-binding patterns of CPNs were heterogenous in each case. The alternation of the glycosialylation and/or acquisition of binding sites for some lectins was sometimes observed through a neoplastic transformation.

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Studies of lectin binding to normal and neoplastic lymph nodes. II. Non-Hodgkin's lymphoma

Cited by 2 publications

References 16 publications

Studies of lectin binding to normal and neoplastic lymphoid tissues. I. Normal nodes and Hodgkin's disease

Studies of lectin binding to normal and neoplastic lymphoid tissues. I. Normal nodes and Hodgkin's disease

Comprehensive lectin histochemistry of normal and neoplastic human choroid plexus cells: alternation of lectin-binding patterns through neoplastic transformation

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