Studies of Methionine Synthesis in Coprinus lagopus

Clark, Erica; Rowbury, R. J.

doi:10.1099/00221287-36-3-333

Cited by 10 publications

(5 citation statements)

References 5 publications

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“…One may postulate that methionine causes the accumulation of high concentrations of pool metabolites which are modified by shunt metabolism to form antibiotics. Clark and Rowbury (4) reported that methionine reduced growth 0 24 48 72 96 and stimulated overall amino acid synthesis in Coprinus. Our data, however, with the exception HOURS of certain sulfur-containing amino acids, showed 4.…”

Section: Discussion Umentioning

confidence: 99%

“…Clark and Rowbury (4) reported that methionine reduced growth 0 24 48 72 96 and stimulated overall amino acid synthesis in Coprinus. Our data, however, with the exception HOURS of certain sulfur-containing amino acids, showed 4. Influence of methionine and sulfate on oxy-no excess of amino acids in methionine-grown ake (A) and carbon dioxide liberation (B).…”

Section: Discussion Umentioning

confidence: 99%

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Effect of Methionine and Sulfate on the Metabolism of Cephalosporium acremonium

Caltrider

Huber

Day

1968

Applied Microbiology

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The metabolism of Cephalosporium acremonium grown in a complex medium supplemented with DL-methionine or inorganic sulfate was studied. More growth occurred in a sulfate medium than in a methionine medium. Methionine-grown cells had an increased rate of respiration, a higher rate of catabolism with acetate and glucose as substrate, and higher specific activities of certain respiratory enzymes than sulfate-grown cells. Labeled acetate and glucose were assimilated at a faster rate by methionine-grown cells than sulfate-grown cells. Taurine, cystathionine, and small quantities of four acidic compounds were present in the amino acid pool of methionine-grown cells, but they were not detected in the pool of sulfate-grown cells. The differences in metabolic activity of sulfate and methionine-grown cells are discussed in regard to cephalosporin C synthesis.

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Section: Discussion Umentioning

confidence: 99%

Section: Discussion Umentioning

confidence: 99%

Effect of Methionine and Sulfate on the Metabolism of Cephalosporium acremonium

Caltrider

Huber

Day

1968

Applied Microbiology

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“…79:36P, 1961). There is good evidence, however, that the formation of cysteine from methionine is not an irreversible process in fungi (5,11,14).…”

Section: Discussionmentioning

confidence: 99%

“…Active transamination would be required for the synthesis of high levels of cysteine, valine, and aminoadipic acid. Clark and Rowbury (5) reported that methionine in Coprinus reduces growth and stimulates overall amino acid synthesis, and excretes excess amounts of amino acids. In our experiments, fermentations with added methionine maintained a neutral pH, and the mycelium was swollen, irregular, and fragmented.…”

Section: Discussionmentioning

confidence: 99%

Role of Methionine in Cephalosporin Synthesis

Caltrider¹,

Niss²

1966

Applied Microbiology

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Methionine has an almost unique stimulatory effect on biosynthesis of cephalosporins (by Cephalosporium acremonium). No other sulfur-containing compound tested, except DL-methionine-DL-sulfoxide, replaced methionine. DL-Methionine stimulated the synthesis of cephalosporins when added after the growth phase. The utilization of inorganic sulfate was repressed by methionine. Experiments with L-methionine-S35 showed that essentially all the sulfur in the cephalosporins was derived from methionine. Sulfur-labeled compounds found in the soluble pool from cells grown with methionine-S35 were methionine, homocysteine, taurine, cystathionine, cysteic acid, glutathionine, and cysteine. DL-Serine-3-CI4 was incorporated into the antibiotics, and its utilization was stimulated by methionine. L-Cysteine had a sparing effect on the incorporation of methionine-S35 and serine-C14 into the antibiotics. The data are consistent with the hypothesis that a cystathionine-mediated pathway is operative in the transfer of sulfur between methionine and cysteine.

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“…The phenylalanine incorporated by TCI should be all, or almost all, derived from the exogenous supply, whereas that incorporated from F, should be almost wholly derived from the main carbon source, i.e. glucose (Roberts et al 1955;Clark & Rowbury, 1964).…”

Section: Glucose Consumptionmentioning

confidence: 99%

Impaired Regulation of Aromatic Amino Acid Synthesis in a Mutant Resistant to p-Fluorophenylalanine

Barker

Lewis

1974

Journal of General Microbiology

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S U M M A R YCoprinus Zagopus is inhibited by the analogue of phenylalanine, p-fluorophenylalanine (PFPA). Mutant strain F,, selected for growth on pFPA, is a slow grower which is not inhibited by the analogue. Over a prolonged period of growth, the amount of [14C]pFPA incorporated into the trichloroacetic acid-insoluble fraction of resistant F7 mycelium is significant, but is less than 20 % of the amount incorporated into similar fractions of the mycelium of the parental sensitive strain TCI. Resistant F7, grown on minimal medium, excretes into the medium p-hydroxyphenylpyruvic acid, phenylpyruvic acid and a number of unidentified phenolic compounds. The sensitive TCI strain grown on minimal medium does not excrete, but when grown on media supplemented with phenylalanine or shikimate excretes traces of these compounds. The total biosynthesis of phenylalanine over 24 h by sensitive strain TCI is greatly reduced by the presence of exogenously supplied phenylalanine. No such reduction in synthesis is observed in the resistant mutant F,. It is inferred that the mutation impairs the regulation of phenylalanine biosynthesis, probably by the loss of feedback control at either the 3-deoxy-~-arabino-heptulosonate 7-phosphate (DAHP) synthetase or chorismate mutase.

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Studies of Methionine Synthesis in Coprinus lagopus

Cited by 10 publications

References 5 publications

Effect of Methionine and Sulfate on the Metabolism of Cephalosporium acremonium

Effect of Methionine and Sulfate on the Metabolism of Cephalosporium acremonium

Role of Methionine in Cephalosporin Synthesis

Impaired Regulation of Aromatic Amino Acid Synthesis in a Mutant Resistant to p-Fluorophenylalanine

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