Studies of odd bases in yeast mitochondrial tRNA: II. Characterization of rare nucleosides

Base composition of mitochondrial RNA species and characterization of mitochondrial 4 S RNA from Locusta migratoria

“…In some respects, our results are similar to those reported recently for the tRNAs from yeast mitochondria [14][15][16]. Further work is intended to characterize locust mitochondrial DNA and its hybridizing properties for locust mitochondrial tRNA.…”

“…Recently, detailed studies demonstrated that yeast mitochondria contain a full complement of endogenously specified tRNAs [13][14][15][16]. With respect to higher animal cells, which possess only a small mitochondrial genome, the question remains open whether all of the nucleic acids taking part in mitochondrial protein biosynthesis are manufactured within the mitochondria or whether some of them have to be imported from the cytoplasm.…”

The nucleotide sequence of yeast mitochondrial histidine‐tRNA

“…Yeast mt tRNAHb contains 8 rare nucleosides. Unlike yeast mt tR.NAmie [S], it has a ~bothy~d~c residue in position 53 as expected from our earlier results [4] showing that total yeast mt tRNA contains almost one residue of ribo~ymi~ne per molecule. mt tRNAHk contains a m'G residue in the 3'-position next to the anticodon, as is the case for yeast mt tRNAfie [5], tRNAyet and tRNAp (our unpublished results).…”

“…The ~to~ondri~ tRNAs (mt tRNAs) have a special base composition, with a very low G t C content; they also show less posttranscriptional modifications than the corresponding cytoplasmic tRNAs [4]. The nucleotide sequence determinations of mt tRNAs from yeast and from Neurospora crassa showed unique structural features [.5,6].…”

Synthesis of the Anticodon HairpintRNAfMet ContainingN-{[9-(β-D-Ribofuranosyl)-9H-purin-6-yl]carbamoyl}-L-threonine (=N6-{{[(1S,2R)-1-Carboxy-2-hydroxypropyl]amino}carbonyl}adenosine, t6A)

“…H (npe)); 7.92 (d, J 8.5, 2 arom. H (npe)); 8.32,8.48 (2s, HÀC(2), HÀC(8)); 8.61 (br. s, NHÀC(6)); 10.03 (d, J 9.0, NHÀC(a)) 13.…”