Studies of plasma volume, red cell volume and total blood volume in young growing rats

Belcher, Edward; Harriss, E. B.

doi:10.1113/jphysiol.1957.sp005875

Cited by 105 publications

(57 citation statements)

References 25 publications

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“…Our value for plasma volume was a little higher than that obtained by Belcher & Harris (1957), but the animals which we used were of lower body weight. Travnickova & Heller (1963), using Evans blue, obtained a plasma volume figure of 5 40 ml./100 g ± 0-05; blood volume 7-61 ml./100 g ± 0-17 for rats 629 B. MORRIS AND R. MORRIS between 25 and 30 g body weight.…”

Section: Discussionmentioning

confidence: 48%

“…The interval between injection and sampling must relate to the nature of the injected material, intervals ranging from 2 min after the injection of hemoglobin (Lippman, 1947) to 2 hr for 59Fe-labelled red cells (Berlin, Huff, van Dyke & Hennessey, 1949) have been used. Belcher & Harris (1957) used U3lI-labelled human albumin and sampled 10 min after injection. Their results showed no serious discrepancy compared with the values which they obtained from their more extensive studies using Evans blue and sampling 5 min after injection.…”

Section: Discussionmentioning

confidence: 99%

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Quantitative assessment of the transmission of labelled protein by the proximal and distal regions of the small intestine of young rats.

Morris¹,

Morris²

1976

The Journal of Physiology

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SUMMARY1. The plasma volume in rats aged 15-16 days was measured by dilution analysis using homologous, 1251-labelled immunoglobulin G. A mean plasma volume of 5-53 ml./100 g and a mean blood volume of 8-01 ml./100 g were obtained.2. After the injection of labelled immunoglobulin G into the heart, homogenates of various abdominal organs and of the carcass were prepared. Labelled immunoglobulin G left the vascular compartment at a rate of about 9-10 %/hr over a 3 hr period. About 11 % of the labelled immunoglobulin G was catabolized in 2 hr.3. The data obtained from these studies was used to make quantitative estimates of the amount of intact immunoglobulin G transmitted from the proximal intestine and from the ileum after the intra-intestinal injection of 1000 ug of labelled immunoglobulin G. Homogenates of the experimental animals were prepared and it was estimated that over 40 % of the labelled immunoglobulin G was transmitted as intact protein from the proximal intestine. The results suggest that no intact immunoglobulin G was transmitted from the ileum, but about 15 % of the protein removed from the ileum was recovered in the whole body as degraded fragments precipitable with trichloroacetic acid.4. These observations are discussed in the context of the transmission of antibodies, and their relevance to the receptor hypothesis is considered.

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Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Quantitative assessment of the transmission of labelled protein by the proximal and distal regions of the small intestine of young rats.

Morris¹,

Morris²

1976

The Journal of Physiology

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“…He found, as did Belcher & Harriss (1957), a decrease in blood volume from 700 at birth to about 4°500 by the time the animal had reached a body weight of 370 g. However, Garcia, unlike Belcher & Harriss, recorded a rise in red cell volume of from 1-4 to 2 1% corresponding with an increase in body weight from 30 to 260 g. This compares with a rise of from 1-5 to 2-6 ,h recorded in the present paper. It must be pointed out that no mention is made by Garcia of any allowance being made for trapped plasma or body haematocrit.…”

Section: Ratsmentioning

confidence: 70%

“…When estimating plasma volumes in rats, Belcher & Harriss (1957) injected, apparently regardless of the size of the animal, 1 mg of Evans blue dye. This amount seems very large, especially for small rats, where the excess dye may be lost from circulation.…”

Section: Ratsmentioning

confidence: 99%

Changes in blood volume and blood picture during the life of the rat and guinea‐pig from birth to maturity

Constable¹

1963

The Journal of Physiology

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“…After a 10-minute equilibration period, three 0.2-ml blood samples were drawn, and the mean number of counts was used to compute plasma volume; adjustment was made for plasma trapping. 23 Statistical significance was determined with analysis of variance, and specific comparisons were made using linear contrasts. 24 Results in DS and DR were compared for each NaCl intake, and 1% and 7% NaCl intakes were compared for each strain.…”

Section: Methodsmentioning

confidence: 99%

Failure of salt loading to inhibit tissue norepinephrine turnover in prehypertensive Dahl salt-sensitive rats.

et al. 1988

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SUMMARY To determine if alterations of electrolyte balance or sympathetic nervous system activity are present in Dahl salt-sensitive rats (DS) before the onset of hypertension, we compared electrolyte balances, extracellular fluid volume (inulin space), plasma volume (radiolabeled albumin), and norepinephrine turnover in peripheral tissues (heart and interscapular brown fat) in prehypertensive DS and Dahl salt-resistant rats (DR). Animals were maintained for 5 to 7 days on either a "normal" or high NaCl diet. Tissue norepinephrine turnover was evaluated by measuring the rate at whkh norepinephrine content decreased following tyrosine hydroxylase inhibition with a-methyl-p-tvrosine. Blood pressure was higher (p < 0.05) hi DS (135 ± 2 [SE] mm Hg) than hi DR (129 ± 2 mm Hg) and was not affected by the diets. Extracellular fluid volume and net Na + and Cl~ balances did not differ between DS and DR. However, plasma volume was greater hi DS than in DR (p < 0.05). In both fat and heart, norepinephrine turnover was decreased by dietary NaCl loading hi DR (p < 0.01), but not hi DS. Thus, the tendency of the DS to become hypertensive with high NaCl intake may be related to the combined effects of an increased plasma volume and the failure of high dietary NaCl to inhibit peripheral sympathetic nervous system activity. 1 -2 The blood pressure of the Dahl saltsensitive rat (DS) rises with increased NaCl intake, whereas that of the Dahl salt-resistant rat (DR) does not. DS excrete sodium less efficiently than do DR according to some 3 but not all 4 investigators, and evidence for an expansion of the extracellular fluid volume in the pathogenesis of hypertension in this salt-sensitive model is conflicting. 5 ' 6 Alternatively, there is increasing evidence for the participation of the nervous system in the pathogenesis of salt-sensitive hypertension. Lesions of the paraventricular nuclei or the anteroventral third ventricle region protect against the development of hypertension in the DS and other models of saltsensitive hypertension 7 -8 ; chemical sympathectomy with guanethidine or 6-hydroxydopamine also prevents hypertension in DS fed a high NaCl diet.9 -10

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Studies of plasma volume, red cell volume and total blood volume in young growing rats

Cited by 105 publications

References 25 publications

Quantitative assessment of the transmission of labelled protein by the proximal and distal regions of the small intestine of young rats.

Quantitative assessment of the transmission of labelled protein by the proximal and distal regions of the small intestine of young rats.

Changes in blood volume and blood picture during the life of the rat and guinea‐pig from birth to maturity

Failure of salt loading to inhibit tissue norepinephrine turnover in prehypertensive Dahl salt-sensitive rats.

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