Studies of specific gene induction during apoptosis of cell lines conditionally immortalized by SV40

doi:10.1016/0014-5793(95)01157-a

FEBS Letters

1995

DOI: 10.1016/0014-5793(95)01157-a

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Studies of specific gene induction during apoptosis of cell lines conditionally immortalized by SV40

Abstract: Inactivation of SV40 large T antigen in cells immortalized with conditional mutants leads to activation of p53 and apoptosis. We have analysed during this process the expression of genes induced by p53 or differentially expressed during apoptosis in other systems. We find an early induction of WafllCipl. We also observe clusterin is induced during the process and displays a high level of expression in non-apoptotic cells, suggesting a protective role for clusterin. Other genes associated with thymocyte and lym… Show more

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Cited by 8 publications

References 30 publications

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Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG‐12

Takasaki

Takarada

Yasuda

et al. 2007

J of Cellular Biochemistry

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The purpose of the present study is to establish and characterize a conditionally immortalized astrocyte cell line and to clarify the genetic networks responsible for the cell growth arrest and differentiation. A conditionally immortalized astrocyte cell line, RCG-12, was established by infecting primary cultured rat cortical glia cells with a temperature-sensitive simian virus 40 large T-antigen. At a permissive temperature of 33 degrees C, the large T-antigen was expressed and cells grew continuously. On the other hand, the down-regulation of T-antigen at a non-permissive temperature of 39 degrees C led to growth arrest and differentiation. The cells expressed astrocyte-expressed genes such as glial fibrillary acidic protein. Interestingly, the differentiated condition induced by the non-permissive temperature significantly elevated the expression levels of several astrocyte-expressed genes. To identify the detailed mechanisms by which non-permissive temperature-induced cell growth arrest and differentiation, we performed high-density oligonucleotide microarray analysis and found that 556 out of 15,923 probe sets were differentially expressed 2.0-fold. A computational gene network analysis revealed that a genetic network containing up-regulated genes such as RB, NOTCH1, and CDKN1A was associated with the cellular growth and proliferation, and that a genetic network containing down-regulated genes such as MYC, CCNB1, and IGF1 was associated with the cell cycle. The established cell line RCG-12 retains some characteristics of astrocytes and should provide an excellent model for studies of astrocyte biology. The present results will also provide a basis for understanding the detailed molecular mechanisms of the growth arrest and differentiation of astrocytes.

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Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG‐12

Takasaki

Takarada

Yasuda

et al. 2007

J of Cellular Biochemistry

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Genes involved in nonpermissive temperature-induced cell differentiation in Sertoli TTE3 cells bearing temperature-sensitive simian virus 40 large T-antigen

Tabuchi

Kondo

Suzuki

et al. 2005

Biochemical and Biophysical Research Communications

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Functional characterization of a conditionally immortalized mouse epididymis caput epithelial cell line MEPC5 using temperature-sensitive simian virus 40 large T-antigen

Tabuchi

Toyama

Toshimori

et al. 2005

Biochemical and Biophysical Research Communications

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Studies of specific gene induction during apoptosis of cell lines conditionally immortalized by SV40

Cited by 8 publications

References 30 publications

Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG‐12

Identification of genetic networks involved in the cell growth arrest and differentiation of a rat astrocyte cell line RCG‐12

Genes involved in nonpermissive temperature-induced cell differentiation in Sertoli TTE3 cells bearing temperature-sensitive simian virus 40 large T-antigen

Functional characterization of a conditionally immortalized mouse epididymis caput epithelial cell line MEPC5 using temperature-sensitive simian virus 40 large T-antigen

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