Studies of Testicular and Epididymal Damage in Relation to the Occurrence of Antisperm Antibodies

Hargreave, T. B.; Busuttil, A.; Elton, R. A.; Harvey, Joanne E.; Chan, Alan; Chisholm, G. D.

doi:10.1111/j.1464-410x.1982.tb13645.x

Cited by 18 publications

(8 citation statements)

References 17 publications

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“…A very important role of the blood-testis barrier is to prevent the production of antisperm antibodies. However, Hargreave et al 24 reported that there was no association between damage to the seminiferous tubules and the presence of agglu-Blastogenic responses to Con A "Data were obtained from two different experiments and expressed as mean cpm ± S.D. of triplicate cultures.…”

Section: Discussionmentioning

confidence: 99%

Effects of Mouse Testicular Extract on Immunocompetent Cells

Emoto¹,

Yagyu²,

Nishikawa³

et al. 1989

American J Rep Immunol

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We investigated mouse testicular extract (TE) to clarify its biological functions in reproductive immunity. TE, at concentrations of 50-300 micrograms/ml, enhanced macrophage activities of spreading, glucose consumption, and cytostasis against a susceptible tumor cell line. On the other hand, TE inhibited concanavalin A (Con A)-induced T-cell blastogenesis in the dose range of 10-600 micrograms/ml. To elucidate the origin of TE, W/Wv mice, which genetically lack germ cells, were used. TE obtained from W/Wv mice enhanced the spreadability of macrophages and inhibited Con A-induced blastogenesis of T cells. The enhancement of macrophage spreading was only achieved by the interstitial fluid (IF), while the suppression of Con A-induced T-cell responses was detected in seminiferous tubule fluid (STF) as well as in IF. TE did not affect listerial antigen-specific responses of lymphocytes in vitro. These results suggest that TE has the capacity to regulate the biological responses associated with reproduction.

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Section: Discussionmentioning

confidence: 99%

Effects of Mouse Testicular Extract on Immunocompetent Cells

Emoto¹,

Yagyu²,

Nishikawa³

et al. 1989

American J Rep Immunol

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“…Some evidence linking antisperm antibodies and damage to the "blood-testis'' barrier has been reported (Hargreave et al, 1982). However, antisperm antibodies may also occur without histological evidence of damage, suggesting some limitation to the concept of this mechanical barrier.…”

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confidence: 99%

Intraepithelial Lymphocytes in the Normal Epididymis. A Mechanism for Tolerance to Sperm Auto‐Antigens?

Ritchie

Hargreave

James

et al. 1984

British Journal of Urology

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Using a monoclonal immunoperoxidase technique on frozen tissue sections of normal testis and epididymis, a distinctive distribution of lymphocytes has been identified. Almost all of the intra-epithelial lymphocytes were T cells and the majority of these belonged to the T8+ ("suppressor/cytotoxic") subset. The distribution contrasted with the predominance of the T4+ ("helper/inducer") subset in the interstitial tissues and in normal peripheral blood. The predominance of the "suppressor/cytotoxic" subset within the epithelium of the epididymis may exist in order to prevent the development of the immune response to auto-antigens on spermatozoa.

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“…Phadke observed epithelial breach, sperm extravasation, macrophages and interstitial inflammatory cells [13]. Hargreave et al observed interstitial infiltration by lymphocytes, macrophages and plasma cells with fibrosis and perivasculitis [20]. Phadke also observed ingestion of sperms by macrophages [13].…”

Section: Discussionmentioning

confidence: 99%

Local Cell Mediated Immune Reaction in Primary Obstructive Male Infertility

Sarda¹,

Bhalla²,

Pandey³

et al. 2011

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Increased intra-epididymal pressure due to obstruction causes a breach in the epididymal lining exposing the highly antigenic sperms to the interstitium. When the exposure is small and intermittent, it is likely to induce a local humoral response which may affect sperm maturation and also react with the epididymal epithelium producing irreversible histological changes. The present study on 30 patients of primary obstructive infertility found direct and indirect evidence of the production of antisperm antibodies locally. ELISA for antisperm antibodies was positive in the epididymal fluid in 16/30 (53%) of the patients. Indirect evidence of the role of local antigen-antibody reaction in the epididymis is apparent in 22/30 (73%) patients who had a lymphocytic infiltrate. The local presence of antisperm antibodies in the epididymal fluid correlated well with the presence of lymphocytic infiltration in the interstitium (p < 0.05). In our study 16/30 patients had a positive ELISA for antisperm antibody and all these patients had interstitial lymphocytic infiltration. In addition eight patients with a negative ELISA also showed interstitial lymphocytic infiltration. Thus 22/30 (73%) patients had an evidence of a local immune reaction directly in the form of a positive ELISA for antisperm antibodies in the epididymal fluid and / or indirect evidence in the form of lymphocytic infiltrate in the interstitium. None of the five controls had either a positive ELISA or lymphocytic infiltrate, this difference was found to be statistically significant (p < 0.005)

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Studies of Testicular and Epididymal Damage in Relation to the Occurrence of Antisperm Antibodies

Cited by 18 publications

References 17 publications

Effects of Mouse Testicular Extract on Immunocompetent Cells

Effects of Mouse Testicular Extract on Immunocompetent Cells

Intraepithelial Lymphocytes in the Normal Epididymis. A Mechanism for Tolerance to Sperm Auto‐Antigens?

Local Cell Mediated Immune Reaction in Primary Obstructive Male Infertility

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