Studies of the Acellular Bone of Teleost Fish

Moss, Melvin L.

doi:10.1159/000141794

Cited by 121 publications

(41 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the basis of function (activity, rate of matrix synthesis) and morphology, osteoblasts are distinct from osteocytes, even though osteocytes are derived from osteoblasts. Acellular bone forms either by osteoblasts depositing bone matrix periosteally, avoiding entrapment (Hall 1978;Weiss and Watabe 1979;Ekanayake and Hall 1987) or by becoming buried as osteocytes that are obliterated through matrix calcification (Moss 1961).…”

Section: Introductionmentioning

confidence: 99%

Ectopic ossicles associated with metacercariae of Apophallus brevis (Trematoda) in yellow perch, Perca ftavescens (Teleostei): development and identification of hone and chondroid bone

et al. 1994

View full text Add to dashboard Cite

This paper describes the development and tissues in mineralized ossicles in the musculature of Perca flavescens infected with metacercariae of the trematode Apophallus brevis. Analysis involved light microscopy, transmission and scanning electron microscopy, X-ray scanning electron microprobe analysis, and tetracycline labelling. Two to 14 days post-infection, fibroblast-like host cells stream towards the parasite cyst forming a fusiform cellular capsule. By 14 days post-infection the capsule differentiates into an inner hypertrophied layer, an extensive middle layer of fibroblast-like cells, and a thin outer layer of flattened fibroblast-like cells forming a fibrous sheath at the capsule/muscle interface. From 21-35 days post-infection, a bony tissue is deposited periosteally in an equatorial ring around the cyst. With time, additional tissue is secreted over the ring increasing its thickness and advancing the matrix front towards the poles of the ossicle. Plump osteoblast-like cells cover the developing ossicle and may become trapped within the matrix in lacunae encapsulated by collagen. By 63 days post-infection, medium-sized ossicles are morphologically similar to large cysts from perch captured in the wild; ovoid with two polarized canals, but lacking acellular or lamellar bone-like tissue. Mineralized ossicles contain calcium, phosphorus and oxygen. Large ossicles retrieved from perch given multiple doses of tetracycline revealed discrete fluorescent bands, indicative of incremental growth. Fully developed ossicles are composed of two skeletal tissues, an inner region of chondroid bone and an outer region of acellular, lamellar bone.

show abstract

Section: Introductionmentioning

confidence: 99%

Ectopic ossicles associated with metacercariae of Apophallus brevis (Trematoda) in yellow perch, Perca ftavescens (Teleostei): development and identification of hone and chondroid bone

et al. 1994

View full text Add to dashboard Cite

show abstract

“…Even more surprising was the discovery that the anosteocytic state is derived: the bones of less advanced fish like the basal teleosts contain numerous osteocytes, while it is the advanced teleosts (more than half of all extant fish) that totally lack osteocytes. It was suggested, therefore, that the loss of osteocytes confers some advantage on the skeleton of advanced teleosts (Moss, 1961). However, the nature of this putative advantage remains elusive.…”

Section: Introductionmentioning

confidence: 99%

The response of anosteocytic bone to controlled loading

Atkins

Milgram

Weiner

et al. 2015

Journal of Experimental Biology

View full text Add to dashboard Cite

The bones of the skeleton of most advanced teleost fish do not contain osteocytes. Considering the pivotal role assigned to osteocytes in the process of modeling and remodeling (the adaptation of external and internal bone structure and morphology to external loads and the repair of areas with micro-damage accumulation, respectively) it is unclear how, and even whether, their skeleton can undergo modeling and remodeling. Here, we report on the results of a study of controlled loading of the anosteocytic opercula of tilapia (Oreochromis aureus). Using a variety of microscopy techniques we show that the bone of the anosteocytic tilapia actively adapts to applied loads, despite the complete absence of osteocytes. We show that in the directly loaded area, the response involves a combination of bone resorption and bone deposition; we interpret these results and the structure of the resultant bone tissue to mean that both modeling and remodeling are taking place in response to load. We further show that adjacent to the loaded area, new bone is deposited in an organized, layered manner, typical of a modeling process. The material stiffness of the newly deposited bone is higher than that of the bone which was present prior to loading. The absence of osteocytes requires another candidate cell for mechanosensing and coordinating the modeling process, with osteoblasts seeming the most likely candidates.

show abstract

“…However, osteichthyans can lack trapped cells and/or mineral material, illustrating the expression of the great adaptive trends of bone in the various osteichthyan lineages (Meunier and Huysseune 1992). More than half of all teleost species have acellular bone, which has similar histological features to those of cellular bone, except that osteocytes are missing because the cells are not incorporated into the osseous matrix (Moss 1961(Moss , 1963. Moreover, bone is vascularised and is submitted to the processes of resorption and reconstruction (Meunier and Huysseune 1992).…”

Section: Introductionmentioning

confidence: 99%

The rocker bone: a new kind of mineralised tissue?

et al. 2008

View full text Add to dashboard Cite

In some Ophidiiform fishes, the anterior part of the swimbladder is thickened into a hard structure called the "rocker bone", which is thought to play a role in sound production. Although this structure has been described as cartilage or bone, its nature is still unknown. We have made a thorough analysis of the rocker bone in Ophidion barbatum and compared it with both classical bone and cartilage. The rocker bone appears to be a new example of mineralisation. It consists of (1) a ground substance mainly composed of proteoglycans (mucopolysaccharide acid) and fibres and (2) a matrix containing small mineralised spherules composed of a bioapatite and fibrils. These spherules are embedded in mineralised cement of a similar composition to the spherules themselves. The rocker bone grows via the apposition of new apatite spherules at its periphery. These spherules are first secreted by the innermost fibroblast layer of the capsule contained in the rocker bone and then grow extracellularly. Blood vessels, which represent the only means of transport for matrix and mineral material, are numerous. They enter the rocker bone via the hyle and ramify towards the capsule. We propose to call this new kind of mineralised tissue constituting the rocker bone "frigolite" (the Belgian name for styrofoam) in reference to the presence of spherules of different sizes and the peculiarity of the rocker bone in presenting a smooth surface when fractured.

show abstract

Studies of the Acellular Bone of Teleost Fish

Cited by 121 publications

References 9 publications

Ectopic ossicles associated with metacercariae of Apophallus brevis (Trematoda) in yellow perch, Perca ftavescens (Teleostei): development and identification of hone and chondroid bone

Ectopic ossicles associated with metacercariae of Apophallus brevis (Trematoda) in yellow perch, Perca ftavescens (Teleostei): development and identification of hone and chondroid bone

The response of anosteocytic bone to controlled loading

The rocker bone: a new kind of mineralised tissue?

Contact Info

Product

Resources

About