Studies of the Antigenic Structure of Mycobacteria

Jensen, K. A.; Kiær, I.; Lundberg, L.

doi:10.1111/apm.1966.66.1.79

Cited by 6 publications

(1 citation statement)

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“…The anti-H37Rv sera that were originally prepared for other types of investigations have been used instead of anti-BCG sera because many workers (11,12,23) have failed to show antigenic differences between human and bovine strains of mycobacteria. This antigenic relationship was confirmed in these experiments because the cross-absorption of anti-H37Rv sera with living BCG removed completely the hemagglutinating antibodies.…”

Section: Discussionmentioning

confidence: 99%

Enhancement activity of anti-mycobacterial sera in experimental Mycobacterium bovis (BCG) infection in mice

Forget¹,

Benoît²,

Turcotte³

et al. 1976

Infect Immun

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The passive transfer of rabbit anti-mycobacterial immunoglobulins was directed against either living or soluble extracts of Mycobacterium tuberculosis strain H37Rv, which promotes the multiplication of the BCG strain of M. tuberculosis in the spleen of mice infected with low doses of this latter strain. This enhancing effect was reduced significantly when antisera were absorbed with living BCG. Moreover, such treatment led to the removal of all hemagglutinating antibodies when antisera were tested against either BCG or H37Rv soluble extracts. Therefore, it is most probable that the enhancing effect is related to the presence of mycobacterial antibodies.

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Section: Discussionmentioning

confidence: 99%

Enhancement activity of anti-mycobacterial sera in experimental Mycobacterium bovis (BCG) infection in mice

Forget¹,

Benoît²,

Turcotte³

et al. 1976

Infect Immun

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An antigenic analysis of the mycobacteria, Mycobacterium fortuitum, Myco. kansasii, Myco. phlei, Myco. smegmatis and Myco. tuberculosis

Stanford

Beck

1968

J. Pathol.

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THE genus Mycobacterium contains a number of fairly clearly defined species, which may be obligatory pathogens, opportunistic pathogens or saprophytes, together with an ill-defined group of anonymous strains. So far, the definition of species has relied largely on morphology, cultural appearances, biochemical activity and temperature requirements. The grouping of strains by Runyon (1959) and Marks and Richards (1962) relies on these and related criteria. Information about the antigenic components of a number of species and unclassified strains has been accumulating over the past few years (Parlett and Youmans, 1958; Castelnuovo et al., 1960; Lind, 1961 ; Sushida and Hirano, 1963; Schaefer, 1965; Jensen, Klaer and Lundberg, 1964, 1966a, b andc). This paper records an antigenic analysis of Mycobacterium fortuitum, Myco. kansasii, Myco. phlei, Myco. smegmatis and Myco. tuberculosis made by Ouchterlony's double diffusion-in-agar precipitation technique. MATERIALS AND METRODS OrganismsAntigen extracts were prepared from 25 strains of mycobacteria that included some obtained from the National Collection of Type Cultures (NCTC) and some isolated by us from patients and soil (PGH). They were: Mycobacterium fortuitum strains NCTC 8573t, 946, 1542,2006,2291,2891,3631 and 8697, and PGH strains " Hoey "t, '' Ugoji " and " Soil "t; Myco. kanrarii strains PGH " Brown "t, " Walker ", " Birk " and " Sheppard "; Myco. phlei strains NCTC 8156t, 525, 8151, 8157, 10008 and 10266; Myco. smegmatis strains NCTC 3337, 8159 and 10265; and Myco. tuberculosis strain H37 Rat. Strains marked with an obelus were also used for preparation of antisera. Owing to the risk involved in the work with living bacteria only one representative of Myco. tuberculosis was used.All the strains of Myco. kansasii and the Hoey strain of Myco. fortuitum were isolated from the sputum of patients. Myco. fortuitum strain Ugoji was recovered from a patient's abscess and Myco. fortuitum strain Soil from a sample of garden soil. All strains of Myco. kansasiiexcept strain Walker, which was a single isolation, were clinically signiscant in that they had repeatedly been found in the patient's sputum. Their identification, and that of the Myco. fortuitum cultures isolated by Present address: (1959, 1965). Preparation of antigensCultures were grown at 37°C in I-litre volumes of modified Sauton's medium (Boyden and Sorkin, 1955) in large Carrel or Thompson flasks, and their pellicles were harvested when no further growth appeared to be taking place. The age of the cultures depended on the species used and varied from 1 to 8 wk. Parts of the harvested pellicles were extracted at -20°C in an " X " press (Edebo, 1961).Microscopical examination of the product obtained after three pressings revealed that the cell walls were almost totally broken up. The crushed material was allowed to stand at 4°C and the cleared fluid layer between the surface flocculate and the sediment was then pipetted off. This extract served as antigen and was stored in small volumes at -20°C.

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Demonstration of species-specific fractions in mycobacterial purified protein derivative (PPD) sensitins

Worthington

Kleeberg

1967

Tubercle

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Studies of the Antigenic Structure of Mycobacteria

Cited by 6 publications

References 1 publication

Enhancement activity of anti-mycobacterial sera in experimental Mycobacterium bovis (BCG) infection in mice

Enhancement activity of anti-mycobacterial sera in experimental Mycobacterium bovis (BCG) infection in mice

An antigenic analysis of the mycobacteria, Mycobacterium fortuitum, Myco. kansasii, Myco. phlei, Myco. smegmatis and Myco. tuberculosis

Demonstration of species-specific fractions in mycobacterial purified protein derivative (PPD) sensitins

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