Studies of the Insulin-inhibitory Effect of Human Albumin Fractions*

Alp, Hakan; Recant, Lillian

doi:10.1172/jci105199

Cited by 32 publications

(10 citation statements)

References 20 publications

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“…These observations have recently been confirmed by Alp and Recant (96), who performed similar studies using a wide variety of albumin preparations. Thcse workers studied albumin preparations isolated by thc Cohn procedure (97) as well as a series of preparations isolated by the Debro procedure (98).…”

Section: Possible Causes Of Diabetessupporting

confidence: 58%

A Survey of Diabetes Mellitus

Holcomb

1966

Journal of Pharmaceutical Sciences

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Section: Possible Causes Of Diabetessupporting

confidence: 58%

A Survey of Diabetes Mellitus

Holcomb

1966

Journal of Pharmaceutical Sciences

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“…12 It has been claimed that this factor with anti-insulin activity has a low molecular weight 13 and is bound to albumin; 12 ' 13 hence, the term "synalbumin insulin antagonist." 14 Other investigators 15 ' 10 subsequently confirmed observations by Vallance-Owen of an increase in the "synalbumin insulin antagonist" in plasma from subjects with diabetes mellitus. 14117 However, based upon theoretical objections 18>19 and the experimental findings of Keen and co-workers 20 -21 that TCA-ethanol extracts of plasma contained high concentrations of ILA and virtually no anti-insulin activity, some doubt has been cast upon the existence of a physiological "synalbumin insulin antagonist.…”

Section: Discussionmentioning

confidence: 83%

Studies of Insulin Antagonism: I. An Artifactual Antagonist to Insulin and Plasma Nonsuppressible Insulin-like Activity Occurring in Preparation of “Albumin”

Ensinck¹,

Poffenbarger²,

Hogan³

et al. 1967

Diabetes

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Because ILA was encountered in TCA-ethanol extracts of plasma dialyzed in boiled Visking casing which interfered with measurement of the "synalbumin insulin antagonist" on isolated muscle, a systematic study of the influence of the casing on these factors was undertaken. Plasma from healthy humans was extracted with TCA-ethanol and dialyzed in nonboiled and boiled Visking casing. The extract ("albumin") was tested by measurement of glucose uptake in rat hemidiaphragms, without and with insulin (1,000 /iU./ml.).. Significant amounts of both ILA and anti-insulin activity were detected in both "albumins" at 3 to 5 gm. per cent; however, more ILA and less insulin inhibition was found in "albumin" dialyzed in boiled casing. The magnitude of ILA could not be accounted for by immunoreactive insulin levels in the "albumin," and the ILA was not suppressed by insulin-specific antiserum (NSILA).From dose-response curves measuring glucose uptake and glycogen synthesis, more NSILA was detected in the "albumin" from boiled casing; however, at 3 to 5 gm. per cent concentration, "albumin" dialyzed in either casing caused inhibition of NSILA. Because partially purified NSILA was found to be retained equally by either boiled or nonboiled casing, an antagonist in Yisking casing was sought. A substance from Visking casing with small molecular weight was found to cause insulin inhibition. When nonantagonistic crystalline albumin was dialyzed in nonboiled casing, marked antagonism to both crystalline insulin and partially purified NSILA was demonstrated.It is concluded that an artifactual casing antagonist to both NSILA and insulin is bound to albumin and may contribute to the measurement of the "synalbumin insulin antagonist.

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“…In vitro studies were conducted with paired rat hemidiaphragms (20). Rats (150 g) were fasted overnight, decapitated, and the hemidiaphragms were removed.…”

Section: Introductionmentioning

confidence: 99%