Studies of the Specific Role of the Subunits of Choriogonadotropin for Biological, Immunological and Physical Properties of the Hormone. Digestion of Choriogonadotropin and Its Isolated Subunits with Serine Carboxypeptidase

Abstract

The residues 90-92 can be split off from the C-terminal region of the isolated -subunit of Choriogonadotropin (residues 88-92: -Tyr-Tyr-His-Lys-Ser-OH) by means of serine carboxypeptidase (des-Lys 91 ,Ser 92 -a-subunit; des-(90-92)

Properties of Glycoprotein Hormone Receptors and Post-Receptor Mechanisms

“…Recombination of hCG (or LH) subunits seems to proceed in a two-step process (Bewley et al, 1974;Ingham et aI., 1976) which consists of a rapid physical association of the subunits followed by a slow process which is necessary to fully restore the conformational, immunological and biological properties of hCG (Merz 1984). A recombination product which contains an ct-subunit with a truncated carboxy terminus and a native 13-subunit (des(88-92)-ct + 13-native) has completely lost the receptor binding ability probably due to an improper conformation of the complex (Merz, 1979;Merz and Dörner, 1979).…”