Studies of the voltage-dependent polyspermy block using cross-species fertilization of amphibians

Jaffe, Laurinda A.; Cross, Nicholas L.; Picheral, Bertrand

doi:10.1016/0012-1606(83)90362-7

Cited by 74 publications

(43 citation statements)

References 23 publications

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“…A variety of experiments have shown that fertilization potential mediates the fast block to polyspermy in many organisms, acting to prevent supernumerary sperm from fusing with the oocyte at short times before physical mechanisms of polyspermy block have been established (264,292,266,267; see Ref.…”

Section: Nature and Developmental Function Of Spontaneous Activitymentioning

confidence: 99%

Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells

Moody¹,

Bosma²

2005

Physiological Reviews

345

323

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At specific stages of development, nerve and muscle cells generate spontaneous electrical activity that is required for normal maturation of intrinsic excitability and synaptic connectivity. The patterns of this spontaneous activity are not simply immature versions of the mature activity, but rather are highly specialized to initiate and control many aspects of neuronal development. The configuration of voltage- and ligand-gated ion channels that are expressed early in development regulate the timing and waveform of this activity. They also regulate Ca2+ influx during spontaneous activity, which is the first step in triggering activity-dependent developmental programs. For these reasons, the properties of voltage- and ligand-gated ion channels expressed by developing neurons and muscle cells often differ markedly from those of adult cells. When viewed from this perspective, the reasons for complex patterns of ion channel emergence and regression during development become much clearer.

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Section: Nature and Developmental Function Of Spontaneous Activitymentioning

confidence: 99%

Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells

Moody¹,

Bosma²

2005

Physiological Reviews

345

323

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“…The initial Ca 2þ rise at fertilization gates Ca 2þ -activated Cl À channels, which depolarize the egg membrane (fertilization potential), and block further sperm entry because sperm fusion in Xenopus is voltage-dependent (Jaffe et al, 1983). The slow block is due to Ca 2þ -dependent fusion of cortical granules, which provides the long-term block to polyspermy (Grey et al, 1974;Wolf, 1974).…”

Section: Physiological Roles Of Ca 2r At Fertilizationmentioning

confidence: 99%

Ca²⁺ signaling differentiation during oocyte maturation

Machaca

2007

Journal Cellular Physiology

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Oocyte maturation is an essential cellular differentiation pathway that prepares the egg for activation at fertilization leading to the initiation of embryogenesis. An integral attribute of oocyte maturation is the remodeling of Ca 2þ signaling pathways endowing the egg with the capacity to produce a specialized Ca 2þ transient at fertilization that is necessary and sufficient for egg activation. Consequently, mechanistic elucidation of Ca 2þ signaling differentiation during oocyte maturation is fundamental to our understanding of egg activation, and offers a glimpse into Ca 2þ signaling regulation during the cell cycle.

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“…The current through the Ca2+-dependent Cl-channel of the egg underlies the depolarization that provides the fast polyspermy block during fertilization (Jaffe, Cross & Picheral, 1983). In the oocytes, this channel is also activated by various Ca2+-mobilizing transmitters acting on 'native' or on 'implanted' (by the injection of exogenous mRNA) neurotransmitter receptors, such as acetylcholine (ACh), serotonin, substance P, neurotensin, etc.…”

Section: Introductionmentioning

confidence: 99%

Two calcium‐activated chloride conductances in Xenopus laevis oocytes permeabilized with the ionophore A23187.

Boton¹,

Dascal²,

Gillo³

et al. 1989

The Journal of Physiology

129

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SUMMARY1. Currents evoked by elevated intracellular free Ca2+ in Xenopus laevis oocytes were studied using the two-electrode voltage clamp technique. The elevation in Ca2+ concentration was achieved in three ways: by the use of the divalent cation ionophore A23187; by application of Ca2+-mobilizing neurotransmitters serotonin and acetylcholine (ACh); by the entry of Ca2+ through voltage-dependent channels.2. In most experiments, the membrane was permeabilized to Ca2+ by a 15 min pretreatment with A23187 in a Ca2+-free solution. Exposure of the ionophore-treated oocytes to external Ca2+ elicited an inward current (at holding potentials of -40 to -60 mV). At external Ca2+ concentrations ([Ca2+]) between 0.1 and 1 mm, the current had a time-to-peak of at least 10 s, and slowly decayed over tens of seconds. At [Ca2+] > 2 mm, the inward current had two distinct kinetic components, a fast and transient one (If.t) and a slow one (Islow).3. The main carrier of the Ca2+-evoked inward current was Cl-. Several data indicate the existence of a tetraethylammonium (TEA)-sensitive K+ conductance.No evidence for a Na+ current was found.4. The two components of the Ca2+-evoked inward current in ionophorepermeabilized oocytes, and the two components of the current evoked by ACh and serotonin (the latter in oocytes injected with rat brain RNA but untreated with A23187), were blocked by intracellular injection of the Ca2+ chelator, ethyleneglycolbis-(,8-aminoethyl ether)-N,N,N'N'-tetraacetic acid (EGTA). The two components of these currents displayed different sensitivity to Ca2+ buffering; higher doses of EGTA were necessary to inhibit the slow component than the fast one.5. One to two minutes of treatment with 2 mM-9-anthracene carboxylic acid (9-AC) fully blocked Ca2+-dependent Cl-current evoked by Ca2+ influx through voltagedependent Ca2+ channels in intact (untreated with A23187) oocytes. In ionophoretreated oocytes, block of If.t was observed at holding potentials at which the current was outward (i.e. due to Cl-influx); Islow was inhibited only partially. The block of Ca2+-evoked Cl-efflux by 9-AC developed much more slowly and was less potent. To explain these results, the existence of two sites of 9-AC action is proposed.6. Exposure of the ionophore-permeabilized oocytes to 0-1-0-2 mm [Ca2+] strongly * To whom correspondence should be sent.

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Studies of the voltage-dependent polyspermy block using cross-species fertilization of amphibians

Cited by 74 publications

References 23 publications

Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells

Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells

Ca²⁺ signaling differentiation during oocyte maturation

Two calcium‐activated chloride conductances in Xenopus laevis oocytes permeabilized with the ionophore A23187.

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Studies of the voltage-dependent polyspermy block using cross-species fertilization of amphibians

Cited by 74 publications

References 23 publications

Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells

Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells

Ca2+ signaling differentiation during oocyte maturation

Two calcium‐activated chloride conductances in Xenopus laevis oocytes permeabilized with the ionophore A23187.

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Ca²⁺ signaling differentiation during oocyte maturation