Studies on Alkaline Serine Protease Produced by<i>Bacillus clausii</i>GMBE 22

Kazan, Dilek; Bal, Hulya; Denizci, Aziz Akın; Ozturk, Nurcin Celik; Öztürk, Hasan Ümit; Dilgimen, Aydan Salman; Öztürk, Dilek; Erarslan, Altan

doi:10.1080/10826060902953269

Cited by 9 publications

(1 citation statement)

References 33 publications

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“…There are many reports on the stimulatory effect of cations on alkaline proteases from different Bacillus species . Positive effect of calcium, magnesium, and manganese on the activity of a protease from Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

The influence of copper on alkaline protease stability toward autolysis and thermal inactivation

Ozturk

Kazan

Denizci

et al. 2012

Engineering in Life Sciences

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Alkaline proteases are one of the most important group of enzymes that are indispensable in a number of different industrial sectors. In this work, the effect of copper ions (Cu2+) was investigated for improving the thermostability and hydrolytic performance of Bacillus clausii GMBE 42 alkaline protease at different temperatures (45–65°C). Maximal residual activity was observed in the presence of 5 mM CuCl2. The enzyme was thermoinactivated according to first‐order kinetics. A stabilization effect caused by copper ions was the result of a decrease in both autolysis and thermoinactivation rates. Thermodynamic analysis of the thermoinactivation process showed that Ea,i, ΔGi, and ΔHi values of the enzyme were higher in the presence of copper ions, but there was no measurable change in ΔSi values. These results show the thermostabilizing potential of copper ions on the enzyme. Lower Km values and higher kcat and kcat/Km values were obtained in the presence of copper ions, which is an indication of the nonessential activation of the enzyme by copper ions. Thermodynamic analysis of casein hydrolysis showed that in presence of copper ions Ea, ΔG≠, ΔH≠, ΔnormalGnormalE−normalS≠, and ΔnormalGnormalE−normalT≠ values of enzyme were lower, but there was no change in ΔS≠ values. This is so far the first study that investigates the effect of cations on the basic catalytic and thermodynamic properties of an alkaline serine protease, which may be used to remove protein wastes from various industries such as food and leather processing.

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Section: Resultsmentioning

confidence: 99%

The influence of copper on alkaline protease stability toward autolysis and thermal inactivation

Ozturk

Kazan

Denizci

et al. 2012

Engineering in Life Sciences

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Haloalkaliphilic Bacteria and Actinobacteria from the Saline Habitats: New Opportunities for Biocatalysis and Bioremediation

Singh

Raval

Purohit

et al. 2011

Microorganisms in Environmental Management

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Isolation and characterization of a serine protease-producing marine bacterium Marinomonas arctica PT-1

Yoo

Park

2015

Bioprocess Biosyst Eng

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A serine protease-producing marine bacterial strain named as PT-1 was isolated and identified as a family of Marinomonas arctica, based on molecular characterization of 16S rRNA gene sequence, phylogenetic tree, and fatty acid composition analyses. Optimized culture conditions for growth of the bacterium PT-1 and production of protease (ProA) were determined to be pH 8.0 in the presence of 5 % NaCl, at 37 °C during 24 h of incubation in the presence of 1.0 % skim milk. The molecular weight of the purified ProA was estimated to be 63-kDa as a major band by SDS-PAGE. We were intrigued to find that the activity of ProA was not inhibited by pepstatin A, chymostatin, and leupeptin known as inhibitors for cysteine protease. However, phenylmethylsulfonyl fluoride (PMSF) completely inhibited protease activity, suggesting that the ProA is like a serine protease. To the best of our knowledge, this is the first report on serine protease of Marinomonas species.

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Studies on Alkaline Serine Protease Produced byBacillus clausiiGMBE 22

Cited by 9 publications

References 33 publications

The influence of copper on alkaline protease stability toward autolysis and thermal inactivation

The influence of copper on alkaline protease stability toward autolysis and thermal inactivation

Haloalkaliphilic Bacteria and Actinobacteria from the Saline Habitats: New Opportunities for Biocatalysis and Bioremediation

Isolation and characterization of a serine protease-producing marine bacterium Marinomonas arctica PT-1

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