Studies on Amino Acid Uptake by Ovine Small Intestine

Johns, John T.; Bergen, Werner G.

doi:10.1093/jn/103.11.1581

Cited by 22 publications

(13 citation statements)

References 17 publications

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“…Williams (1969) ranked amino acids in the order in which they were absorbed from intestinal loops in the sheep and observed that this order varied with intestinal location, indicating that affhities for the transport systems differed among sites. Lysine absorption by sheep intestinal rings increased with distance fiom the pylorus, with maximal absorption occurring in the ileum (Johns and Bergen, 1973). but the authors felt that this was an artifact of their in vitro system.…”

Section: Amino Acid Absorptionmentioning

confidence: 95%

“…During the same time period that the everted sac was used, the usefulness of intestinal rings in measuring amino acid absorption became known (Agar et al, 1954). Studies of amino acid absorption from the small intestine of sheep utilizing this technique were reported by Johns and Bergen (1973).…”

Section: Amino Acid Absorptionmentioning

confidence: 99%

“…The complex pattern of absorption of amino acids probably is attributable to differences in the affinities of carrier systems for individual amino acids and the fact that competition for transport is greater among amino acids for which a carrier has a greater affinity (Adibi, 1969). Johns and Bergen (1973) reported a 48% reduction in lysine absorption by ovine intestinal rings when leucine was present at three to four times the lysine concentration. More methionine was absorbed than either threonine or valine from everted sacs of ovine intestine (%Kips et al, 1976) and, when different mixtures of methionine.…”

Section: The Relative Importance Of Na+dependentmentioning

confidence: 99%

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Intestinal absorption of protein hydrolysis products: a review.

Webb

1990

Journal of Animal Science

162

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Many experimental techniques have allowed researchers to probe the fate of hydrolysis products from proteins in the small intestine. An overview of amino acid and peptide absorption from the small intestine is presented with attention given to historical perspectives that have led to current concepts. Speculation about nutritional significance of these processes is offered. Species differences exist in site of amino acid absorption. Numerous mechanisms are available for the transport of amino acids, including Na(+)-dependent carriers (energy-requiring), Na(+)-independent carriers and diffusion. The relative contribution each transport system makes to absorption is dependent on substrate concentration. Individual amino acids are not absorbed with equal efficiency; methionine usually is absorbed in the greatest proportion. There are interactions among amino acids for transports by specific transport systems. Small peptides (mostly di- and tripeptides) are absorbed from the small intestine more rapidly than are free amino acids; peptides are transported by systems independent of those responsible for transporting free amino acids. Evidence exists that the active transport of these peptides is via a proton gradient. Although the concept that peptides are absorbed intact into the circulation is not universally accepted, evidence supporting the possibility of tissue utilization of these small peptides is accumulating.

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Section: Amino Acid Absorptionmentioning

confidence: 95%

Section: Amino Acid Absorptionmentioning

confidence: 99%

Section: The Relative Importance Of Na+dependentmentioning

confidence: 99%

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Intestinal absorption of protein hydrolysis products: a review.

Webb

1990

Journal of Animal Science

162

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“…The use of biodegradable particles such as proteins and of nonbiodegradable particles such as latexes have demonstrated the uptake of orally administrated particles by PPs and their subsequent transportation to the lymph vessels, blood vessels, and mesenteric lymph nodes (Carr et al 1996;Johns and Bergen 1973). In addition, abnormal prion protein (PrP Sc ) was first detected within the PPs of distal ileum in oral bovine spongiform encephalopathy (BSE) challenge (Terry et al 2003;Wells et al 1994), and PrP Sc is generally thought to spread from the GALT to the peripheral nervous system (i.e., neuroinvasion; Espinosa et al 2007;Iwata et al 2006;van Keulen et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer’s patches of calves

et al. 2009

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We have previously reported the early uptake and transport of foreign particles into Peyer's patches (PPs) of newborn and 2-month-old calves and shown that the peak uptake of particles occurs 6 h after inoculation, in addition to site- and size-related effects on particle uptake. We now report the distribution of immune cells within PPs of the distal ileum in newborn and 2-month-old calves inoculated with carbon black. The types of immune cells involved in the early uptake and transport of recombinant mouse prion protein (rMPrP) within PPs of newborn calf were investigated by using monoclonal antibodies CD11c, CD14, CD68, CD172a, and CD21. CD11c(+), CD14(+), CD68(+), CD172a(+), and CD21(+) immune cells were widely distributed in four tissue compartments (villi, dome, interfollicular region, and follicles) of PPs in the distal ileum of newborn and 2-month-old calves, whereas CD11c(+), CD14(+), CD172a(+), and CD21(+) immune cells were more prominently distributed in the dome areas of newborn calves than in 2-month-old calves. Moreover, CD11c(+) and CD14(+) dendritic cells, CD172a(+) and CD68(+) macrophages, and CD21(+) follicular dendritic cells containing rMPrP were primarily observed in the dome and inner follicular regions. The deposition of rMPrP within CD11c(+), CD14(+), CD172a(+), and CD68(+) cells, but not CD21(+) cells, was detected in villous regions. rMPrP-positive immune cells within the interfollicular regions included only CD11c(+) and CD172(+) cells. Although the particles used in this investigation do not include the infectious prion protein, PrP(Sc), our experimental setup provides a useful model for studying immune cells involved in the early uptake and transport of PrP(Sc).

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“…Differences in the rates of absorption of amino acids along the length of the small intestine of sheep have also been noted from studies both in vivo (Williams, 1969;Ben-Ghedalia et al 1974;Tagari & Bergman, 1978) and in vitro (Johns & Bergen, 1973;Phillips et al 1976).…”

mentioning

confidence: 81%

The distribution of peptidase activity in the small intestine of sheep

Richardson¹,

Jouan²

1986

Br J Nutr

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1. The activity of peptidase enzyme(s) was measured in homogenates of mucosa taken at slaughter from sites along the length of sheep small intestine.2. The activity of the homogenate towards six dipeptides showed a similar pattern of distribution for each substrate. Activity was lowest in the proximal duodenum, rising through the jejunum to peak in the mid-ileum and declining towards the terminal ileum. The distribution of activity towards two tripeptides was similar.3. Studies using the inhibitor p-hydroxymercuribenzoate showed that the activity towards the dipeptides was mainly localized in the cytosol fraction, while that towards the tripeptides was evenly distributed between the cytosol and the brush-border and activity towards a tetrapeptide was mainly associated with the brush-border.4. The results are discussed in relation to the site and mechanism of protein digestion and absorption in the ruminant small intestine.

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Studies on Amino Acid Uptake by Ovine Small Intestine

Cited by 22 publications

References 17 publications

Intestinal absorption of protein hydrolysis products: a review.

Intestinal absorption of protein hydrolysis products: a review.

Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer’s patches of calves

The distribution of peptidase activity in the small intestine of sheep

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