Studies on analgesic, anti-inflammatory activities of stem and roots of Inula cuspidata C.B Clarke

Abstract

The present study was carried out to evaluate analgesic and anti-inflammatory activities in Inula cuspidata stem and root extracts along with heavy metals estimation in stem and root powder.Stem and roots were extracted with chloroform (ICSCE, ICRCE) and methanol (ICSME, ICRME). Acute oral toxicity of all extracts was determined by OECD guidelines 425. Analgesic activity was investigated by using hot plate and acetic acid induced writhing models. Anti-inflammatory activity (acute) of all extracts was evaluated by carrageenan induced paw edema model. In addition, root and stem powder was screened for heavy metals (As, Pb, Cd, Hg) estimation using atomic absorption spectroscopy. In acute toxicity study no mortality was observed when each extract was orally administered with 2.0 g/kg. At the doses (100 and 200 mg/kg) ICRME followed by ICSME showed significant and dose dependent analgesic and anti-inflammatory effects compared with chloroform extracts. The heavy metals concentration in stem and root powders was found to be within the permissible limits as recommended by WHO for herbal raw materials. The findings of the present study validated the folkloric use of Inula cuspidata as analgesic and anti-inflammatory. In addition, the results intimate that heavy metals present in raw material were found to be within the defined limits, and it exhibits that the therapeutic efficacy of plant may not be effected, which can be otherwise possibly effected if the plant sequester high concentration of heavy metals from the polluted environment as well as from the soil rich in pesticides and sewage sludge etc.

Anthelmintic and Analgesic Activities of Trachyspermum Khasianum H. Wolff

“…Rats were immediately removed from the hot plates on paw licking or jumping [ 27 ]. The animals were taken randomly and divided into 6 groups containing 5 animals in each group designated as group-I, group-II, group-III, group-IV, group-V, and group-VI as mentioned below [ 28 ].…”

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“…The reaction time (paw licking or jumping) was recorded for each mice at periods of 30 min, 60 min and 90 min following administration of medicine or vehicle with the cut-off time 15 sec to avoid injury. The rise in reaction time in plant extracts and standard treated groups were compared to those of the control group 22 . Tail Immersion Test: The lower two-thirds of the tail had been engrossed in a beaker comprising water kept at 50 ± 0.5 °C.…”

Preliminary Studies on the Anti-Inflammatory and Analgesic Effects of Methanol Leaf Extract of Ficus asperifolia Miq

“…2,3 Herbal products are extensively used in the management of many inflammatory and painful conditions and recently there is a renaissance of interest in medicinal plants with antiinflammatory and analgesic properties. 4 This could be attributed to not only the accessibility, acceptability, affordability and safety profiles of the medicinal plants 4,5 but also to the adverse effects associated with the currently used orthodox drugs. 6 Ficus asperifolia Miq.…”