Studies on Antirenin

Lamfrom, Hildegard; Haas, Erwin; Goldblatt, Harry

doi:10.1152/ajplegacy.1954.177.1.55

Cited by 43 publications

(28 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Renin fractions 2 and 3 and nonrenin fractions (6)(7)(8)(9)(10)(11) were purified by DEAE-cellulose chromatography followed by passage through a pepstatin affinity column. The antihypertensive effect was evaluated according to the following criteria: -= no effect, + = a significant decrease in blood pressure, + + = a decrease in blood pressure which returned at least halfway to the preclipping control level, and + + + = a decrease in blood pressure to the preclipping level.…”

Section: Effect Of Direct Immunization With Kidney Extracts On the Blmentioning

confidence: 99%

“…Frank (8) was able to lower the blood pressure of hypertensive monkeys by passive transfer of dog serum containing antibody to human renin as well as by direct immunization with human renin. In contrast, Helmer (4) could not lower the blood pressure of hypertensive rats actively immunized with hog renin, nor were Lamfrom et al (9) successful with hypertensive humans treated with hog renin. However, in these latter two cases success should not have been expected, since the host's (rat or human) antibody to hog renin did not cross-react with its own renin.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Chronic one-kidney hypertension in rabbits. I. Treatment with kidney extracts.

Skeggs¹,

Kahn²,

Levine³

et al. 1975

Circulation Research

View full text Add to dashboard Cite

An investigation into the mechanism that sustains the blood pressure in chronic one-kidney hypertension in rabbits was made using passive and active immunization with hog kidney extracts containing renin and with angiotensin antagonists. Seven hypertensive rabbits were passively immunized for extended periods with antiserum prepared in other rabbits. Antirenin levels were in the range of 1-3 units/ml. Control experiments demonstrated that antirenin concentrations of 1.0 unit/ml or more eliminated more than 99% of the pressor response to renin. There was no lowering of blood pressure that could be attributed to the antibodies. No decrease in blood pressure was observed in 13 experiments in 7 rabbits given the angiotensin II antagonist l-Sar-8-Ile-angiotensin II; infusion rates of 0.09-87 ^g/min kg"' were used for periods of a few hours up to 3 days. A reduction in blood pressure occurred in 16 of 19 rabbits immunized directly with extracts containing renin with specific activities of 9.6-757 GU/mg. Plasma antirenin titers correlated poorly with reductions in blood pressure. The blood pressure of 22 rabbits given equal amounts of protein without renin was unaffected. It is concluded that the elevation of blood pressure in rabbits with chronic one-kidney hypertension is not dependent on circulating renin or angiotensin; rather, it results from the presence of renin in an extravascular location or from an unknown pressor substance.

show abstract

Section: Effect Of Direct Immunization With Kidney Extracts On the Blmentioning

confidence: 99%

mentioning

confidence: 99%

Chronic one-kidney hypertension in rabbits. I. Treatment with kidney extracts.

Skeggs¹,

Kahn²,

Levine³

et al. 1975

Circulation Research

View full text Add to dashboard Cite

show abstract

“…Indeed, the early studies on renin inhibition deal with passive or active immunization against renin (2)(3)(4). However, interpretation of such results is limited by the fact that renin was not completely purified.…”

mentioning

confidence: 99%

Active immunization against renin in normotensive marmoset.

Michel¹,

Guettier²,

Philippe³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Primate renins (human and monkey) are very similar. We used pure human renin to immunize marmosets (Calfithrirjacchus) and thereby produce a chronic blockade of the renin-angiotensinogen reaction. After a control period of 2 months, five male marmosets, on their usual sodium-poor diet, were immunized against pure human renin by three subcutaneous injections of 30 ,ug each, with complete and then incomplete Freund's adjuvant. Three The inhibition of the renin-angiotensin system at its first and rate-limiting step, the action of renin on angiotensinogen, can be achieved by the use of synthetic renin inhibitors (1) and by immunological tools. Indeed, the early studies on renin inhibition deal with passive or active immunization against renin (2-4). However, interpretation of such results is limited by the fact that renin was not completely purified. Thus, heterologous renin was used for immunization protocol, even though it is now known that renin has a very strong immunological specificity. Finally, only blood pressure was monitored in the earlier experiments, and no plasma renin activity or aldosterone assays were available. The renin antibody titer also could not be directly measured.The acute passive immunization of renovascular hypertensive dogs (5) and normotensive marmosets (6) with specific homologous renin antibodies clearly showed that the level of blood pressure depended on renin. From this latter study (6) it was concluded that renin regulated blood pressure in the normotensive marmoset ingesting its usual amount of sodium. The present study was designed to examine the effects of active immunization against renin in marmosets and to observe the effects of chronic blockade of the renin substrate reaction. (SBP) was also recorded once a week in unanesthetized animals by the tail-cuff method (BP recorder 8005, W+W Electronic, Appelex, Bagneux, France). The validation of the tail-cuff method for blood pressure measurement has been reported in normotensive (7) and hypertensive marmosets (8). Five marmosets were immunized by three repeated subcutaneous injections of 30 Ag of highly purified human renin (specific activity, 680 Goldblatt units per mg of protein). Purification was performed by immunoadsorption on a monoclonal antibody from ajuxtaglomerular cell tumor as described by Galen et al. (9). The first injections were performed in complete Freund's adjuvant (0.5 ml), the two others in incomplete Freund's adjuvant (0.5 ml). Three control animals received only the adjuvant. Injections were repeated at intervals of 3 weeks. MATERIALS AND METHODSIn Vitro Experiments. The presence of renin antibodies was determined by the ability of marmoset plasma to bind pure human iodinated renin. The titer was defined as the antiserum dilution able to bind 50% of the renin tracer. Plasma renin concentration was measured by radioimmunoassay (RIA) of angiotensin I generated by 1 hr of incubation of 1:50-diluted marmoset plasma in the presence of an excess (635 pmol/200 gl) of human angiotensinogen (6). When the...

show abstract

“…The first extensive purification of renin was done by Lamfrom, Haas and Goldblatt in 1953. 13 From that time on, one is impressed by the number of acknowledgments in publications from all over the world which state: "We are indebted to Drs. Harry Goldblatt and Erwin Haas for their generous The next major advances in the renin-angiotensin story also occurred here in Cleveland when Skeggs and his colleagues isolated and sequenced the decapeptide, angiotensin I, and purified "the hypertensin-converting enzyme" which is responsible for the production of the vasoactive octapeptide, angiotensin II.…”

mentioning

confidence: 99%

The Goldblatt memorial lecture. Part I: Experimental renovascular hyptertension.

Barger¹

1979

Hypertension

View full text Add to dashboard Cite

Studies on Antirenin

Cited by 43 publications

References 0 publications

Chronic one-kidney hypertension in rabbits. I. Treatment with kidney extracts.

Chronic one-kidney hypertension in rabbits. I. Treatment with kidney extracts.

Active immunization against renin in normotensive marmoset.

The Goldblatt memorial lecture. Part I: Experimental renovascular hyptertension.

Contact Info

Product

Resources

About