The purpose of the study was to examine the genetic variability, heritability and genetic advance as a per cent of mean among the six parents selected through principal component analysis and 30 hybrids of desi cotton. The experiment was laid out in during kharif,2022, at the Department of Cotton, TNAU. Five fibre quality traits and 13 yield contributing traits were analysed as a part of genetic variability investigations. With the exception of the uniformity index, ANOVA demonstrated significance for all of the characteristics examined, suggesting that parents and hybrids have adequate amount of variation. An increased environmental influence on these traits is indicated by the fact that the phenotypic coefficient of variation was marginally greater than the genotypic coefficient of variance. Positive and significant correlation with seed cotton yield per plant was observed for plant height, number of sympodia per plant, number of bolls per plant, boll weight and number of seeds per boll which suggested that increase or improvement in these characters lead to improvement in seed cotton yield/ plant. The traits namely, fibre strength, lint index and number of bolls exerted a high direct effect on seed cotton yield while, boll weight and plant height exerted a moderate positive direct effect on seed cotton yield. The hybrids K12 × RG763 and CNA1007 × RG763 can be used for the better attainment of yield as they have highest boll weight along with seed cotton yield per plant and ginning outturn.